Cation transport and energy metabolism in the nucleated erythrocyte of the dogfish shark, Squalus acanthias

James Theodore, Eugene D. Robin, H. Victor Murdaugh, Carroll E Cross

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1. 1. Electrolyte distribution in the nucleated erythrocyte of the dogfish shark, Squalus acanthias, resembles that found in low Na+-high K+ mammalian erythrocytes. 2. 2. Na+ efflux (20°C) averaged 14·9 ± 2·7 m-equiv × kg RBC H2O-1 × hr-1, being reduced significantly by monoiodoacetate (10-2 M), N2 exposure, and ouabain (10-4 M). 3. 3. K+ influx (20°C) averaged 9·1 ±4·0 m-equiv × kg RBC H2O-1× hr-1, being reduced significantly by exposure to 12°C and ouabain (10-4 M). 4. 4. Total O2 consumption (30°C) averaged 3·14 ± 0·76 mM × kg RBC H2O-1× hr-1. Sixty-five per cent of Q́O2 is related to ATP generation. 5. 5. Lactate production (30°C) averaged 1·52 mM × kg RBC H2O-1 × hr-1. Inhibition of oxidative phosphorylation produced a Pasteur effect. 6. 6. Total energy availability exceeds that required for cation transport, suggesting that other energy-consuming processes are present. A substantial amount of energy required for cation transport is presumably derived from oxidative phosphorylation.

Original languageEnglish (US)
Pages (from-to)639-654
Number of pages16
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume42
Issue number3
DOIs
StatePublished - Jul 1 1972
Externally publishedYes

Fingerprint

Squalus acanthias
Dogfish
Sharks
Erythroblasts
Oxidative Phosphorylation
Ouabain
Energy Metabolism
Cations
Electrolytes
Lactic Acid
Adenosine Triphosphate
Erythrocytes
Availability

Keywords

  • antimycin A
  • Cation transport
  • cyanide
  • dinitrophenol
  • electrochemical gradient
  • electrolyte distribution
  • energetics
  • energy cost
  • glycolysis
  • low Na vertebrate erythrocytes
  • monoiodoacetate
  • nitrogen
  • O consumption
  • ouabain
  • oxidative phosphorylation
  • Squalus acanthias

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Biochemistry

Cite this

Cation transport and energy metabolism in the nucleated erythrocyte of the dogfish shark, Squalus acanthias. / Theodore, James; Robin, Eugene D.; Murdaugh, H. Victor; Cross, Carroll E.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 42, No. 3, 01.07.1972, p. 639-654.

Research output: Contribution to journalArticle

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N2 - 1. 1. Electrolyte distribution in the nucleated erythrocyte of the dogfish shark, Squalus acanthias, resembles that found in low Na+-high K+ mammalian erythrocytes. 2. 2. Na+ efflux (20°C) averaged 14·9 ± 2·7 m-equiv × kg RBC H2O-1 × hr-1, being reduced significantly by monoiodoacetate (10-2 M), N2 exposure, and ouabain (10-4 M). 3. 3. K+ influx (20°C) averaged 9·1 ±4·0 m-equiv × kg RBC H2O-1× hr-1, being reduced significantly by exposure to 12°C and ouabain (10-4 M). 4. 4. Total O2 consumption (30°C) averaged 3·14 ± 0·76 mM × kg RBC H2O-1× hr-1. Sixty-five per cent of Q́O2 is related to ATP generation. 5. 5. Lactate production (30°C) averaged 1·52 mM × kg RBC H2O-1 × hr-1. Inhibition of oxidative phosphorylation produced a Pasteur effect. 6. 6. Total energy availability exceeds that required for cation transport, suggesting that other energy-consuming processes are present. A substantial amount of energy required for cation transport is presumably derived from oxidative phosphorylation.

AB - 1. 1. Electrolyte distribution in the nucleated erythrocyte of the dogfish shark, Squalus acanthias, resembles that found in low Na+-high K+ mammalian erythrocytes. 2. 2. Na+ efflux (20°C) averaged 14·9 ± 2·7 m-equiv × kg RBC H2O-1 × hr-1, being reduced significantly by monoiodoacetate (10-2 M), N2 exposure, and ouabain (10-4 M). 3. 3. K+ influx (20°C) averaged 9·1 ±4·0 m-equiv × kg RBC H2O-1× hr-1, being reduced significantly by exposure to 12°C and ouabain (10-4 M). 4. 4. Total O2 consumption (30°C) averaged 3·14 ± 0·76 mM × kg RBC H2O-1× hr-1. Sixty-five per cent of Q́O2 is related to ATP generation. 5. 5. Lactate production (30°C) averaged 1·52 mM × kg RBC H2O-1 × hr-1. Inhibition of oxidative phosphorylation produced a Pasteur effect. 6. 6. Total energy availability exceeds that required for cation transport, suggesting that other energy-consuming processes are present. A substantial amount of energy required for cation transport is presumably derived from oxidative phosphorylation.

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