(+)-Catechin in human plasma after ingestion of a single serving of reconstituted red wine

Jennifer Rc Bell, Jennifer L. Donovan, Rodney Wong, Andrew L. Waterhouse, J. Bruce German, Rosemary L. Walzem, Siddika E Karakas

Research output: Contribution to journalArticle

209 Scopus citations

Abstract

Background: Red wine consumption may decrease the risk of coronary heart disease through the actions of its constituent flavonoids. (+)-Catechin is an abundant flavonoid in red wine. Objective: The objective was to determine changes in plasma (+)-catechin concentrations after ingestion of a single, moderate serving of dealcoholized red wine reconstituted with either water (DRW) or water and alcohol (ARW). Design: Nine subjects (5 men, 4 women) ingested, in random order, 120 mL DRW on one day and 120 mL ARW on another day. Both the DRW and ARW contained 35 mg (121 μmol) free (+)-catechin. Blood samples were collected at 0, 0.5, 1, 2, 3, 4, and 8 h. Plasma was analyzed by gas chromatography-mass spectrometry for (+)-catechin after enzymatic release of sulfate and glucuronide conjugates. Results: Calcium ions were needed to effectively hydrolyze (+)-catechin conjugates in plasma containing EDTA. Neither the ARW or DRW nor sex affected the area under the curve at 8 h, the maximum concentration (C(max)), or the time it took for plasma total (+)-catechin to reach maximum concentration (t(max)). Pooled mean (±SEM) values for the ARW and DRW were as follows: area under the curve, 306.1 ± 29.5 nmol·h/L; c(max), 76.7 ± 7.5 nmol/L; and t(max), 1.44 ± 0.13 h. The half-life of (+)-catechin in plasma was significantly less (P = 0.038) after ingestion of the ARW (3.17 h) than after ingestion of the DRW (4.08 h). Conclusions: Increases in plasma total (+)-catechin concentrations were not significantly different after single moderate servings of either the ARW or DRW. Alcohol in the ARW hastened the elimination of (+)-catechin from the plasma compartment. (+)-Catechin elimination may represent excretion or conversion to methylated derivatives.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Volume71
Issue number1
StatePublished - Jan 2000

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Keywords

  • (+)-Catechin
  • Antioxidant
  • Ethanol
  • Flavonoid
  • Humans
  • Pharmacokinetics
  • Red wine

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Bell, J. R., Donovan, J. L., Wong, R., Waterhouse, A. L., German, J. B., Walzem, R. L., & Karakas, S. E. (2000). (+)-Catechin in human plasma after ingestion of a single serving of reconstituted red wine. American Journal of Clinical Nutrition, 71(1), 103-108.