Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes

Marcela A. Vazquez Prieto, Ahmed Bettaieb, Cecilia Rodriguez Lanzi, Verónica C. Soto, Diahann J. Perdicaro, Claudio R. Galmarini, Fawaz Haj, Roberto M. Miatello, Patricia I. Oteiza

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Abstract

Scope: This study evaluated the capacity of dietary catechin (C), quercetin (Q), and the combination of both (CQ), to attenuate adipose inflammation triggered by high fructose (HFr) consumption in rats and by tumor necrosis factor alpha (TNF-α) in 3T3-L1 adipocytes. Methods and results: In rats, HFr consumption for 6 wk caused dyslipidemia, insulin resistance, reduced plasma adiponectin, adiposity, and adipose tissue inflammation. Dietary supplementation with 20 mg/kg/day of C, Q, and CQ improved all these parameters. In 3T3-L1 adipocytes, C and Q attenuated TNF-α-induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP-1, resistin), and decreased adiponectin. The protective effects of C and Q on adipose inflammation are in part associated with their capacity to (i) decrease the activation of the mitogen-activated kinases (MAPKs) JNK and p38; and (ii) prevent the downregulation of PPAR-γ. In summary, C and Q, and to a larger extent the combination of both, attenuated adipose proinflammatory signaling cascades and regulated the balance of molecules that improve (adiponectin) or impair (TNF-α, MCP-1, resistin) insulin sensitivity. Conclusion: Together, these findings suggest that dietary Q and C may have potential benefits in mitigating MetS-associated adipose inflammation, oxidative stress, and insulin resistance.

Original languageEnglish (US)
Pages (from-to)622-633
Number of pages12
JournalMolecular Nutrition and Food Research
Volume59
Issue number4
DOIs
StatePublished - Apr 1 2015

Fingerprint

Catechin
Quercetin
Fructose
catechin
adipocytes
Adipocytes
quercetin
adiponectin
fructose
Adiponectin
inflammation
resistin
insulin resistance
tumor necrosis factor-alpha
Inflammation
Resistin
Insulin Resistance
Tumor Necrosis Factor-alpha
rats
1-methylcyclopropene

Keywords

  • Adipokines
  • Adipose tissue inflammation
  • Flavonoids
  • High fructose diet
  • Metabolic syndrome

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Vazquez Prieto, M. A., Bettaieb, A., Rodriguez Lanzi, C., Soto, V. C., Perdicaro, D. J., Galmarini, C. R., ... Oteiza, P. I. (2015). Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes. Molecular Nutrition and Food Research, 59(4), 622-633. https://doi.org/10.1002/mnfr.201400631

Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes. / Vazquez Prieto, Marcela A.; Bettaieb, Ahmed; Rodriguez Lanzi, Cecilia; Soto, Verónica C.; Perdicaro, Diahann J.; Galmarini, Claudio R.; Haj, Fawaz; Miatello, Roberto M.; Oteiza, Patricia I.

In: Molecular Nutrition and Food Research, Vol. 59, No. 4, 01.04.2015, p. 622-633.

Research output: Contribution to journalArticle

Vazquez Prieto, MA, Bettaieb, A, Rodriguez Lanzi, C, Soto, VC, Perdicaro, DJ, Galmarini, CR, Haj, F, Miatello, RM & Oteiza, PI 2015, 'Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes', Molecular Nutrition and Food Research, vol. 59, no. 4, pp. 622-633. https://doi.org/10.1002/mnfr.201400631
Vazquez Prieto MA, Bettaieb A, Rodriguez Lanzi C, Soto VC, Perdicaro DJ, Galmarini CR et al. Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes. Molecular Nutrition and Food Research. 2015 Apr 1;59(4):622-633. https://doi.org/10.1002/mnfr.201400631
Vazquez Prieto, Marcela A. ; Bettaieb, Ahmed ; Rodriguez Lanzi, Cecilia ; Soto, Verónica C. ; Perdicaro, Diahann J. ; Galmarini, Claudio R. ; Haj, Fawaz ; Miatello, Roberto M. ; Oteiza, Patricia I. / Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes. In: Molecular Nutrition and Food Research. 2015 ; Vol. 59, No. 4. pp. 622-633.
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AU - Soto, Verónica C.

AU - Perdicaro, Diahann J.

AU - Galmarini, Claudio R.

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