Abstract
Scope: This study evaluated the capacity of dietary catechin (C), quercetin (Q), and the combination of both (CQ), to attenuate adipose inflammation triggered by high fructose (HFr) consumption in rats and by tumor necrosis factor alpha (TNF-α) in 3T3-L1 adipocytes. Methods and results: In rats, HFr consumption for 6 wk caused dyslipidemia, insulin resistance, reduced plasma adiponectin, adiposity, and adipose tissue inflammation. Dietary supplementation with 20 mg/kg/day of C, Q, and CQ improved all these parameters. In 3T3-L1 adipocytes, C and Q attenuated TNF-α-induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP-1, resistin), and decreased adiponectin. The protective effects of C and Q on adipose inflammation are in part associated with their capacity to (i) decrease the activation of the mitogen-activated kinases (MAPKs) JNK and p38; and (ii) prevent the downregulation of PPAR-γ. In summary, C and Q, and to a larger extent the combination of both, attenuated adipose proinflammatory signaling cascades and regulated the balance of molecules that improve (adiponectin) or impair (TNF-α, MCP-1, resistin) insulin sensitivity. Conclusion: Together, these findings suggest that dietary Q and C may have potential benefits in mitigating MetS-associated adipose inflammation, oxidative stress, and insulin resistance.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 622-633 |
| Number of pages | 12 |
| Journal | Molecular Nutrition and Food Research |
| Volume | 59 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2015 |
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Keywords
- Adipokines
- Adipose tissue inflammation
- Flavonoids
- High fructose diet
- Metabolic syndrome
ASJC Scopus subject areas
- Food Science
- Biotechnology
Cite this
Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes. / Vazquez Prieto, Marcela A.; Bettaieb, Ahmed; Rodriguez Lanzi, Cecilia; Soto, Verónica C.; Perdicaro, Diahann J.; Galmarini, Claudio R.; Haj, Fawaz; Miatello, Roberto M.; Oteiza, Patricia I.
In: Molecular Nutrition and Food Research, Vol. 59, No. 4, 01.04.2015, p. 622-633.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes
AU - Vazquez Prieto, Marcela A.
AU - Bettaieb, Ahmed
AU - Rodriguez Lanzi, Cecilia
AU - Soto, Verónica C.
AU - Perdicaro, Diahann J.
AU - Galmarini, Claudio R.
AU - Haj, Fawaz
AU - Miatello, Roberto M.
AU - Oteiza, Patricia I.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Scope: This study evaluated the capacity of dietary catechin (C), quercetin (Q), and the combination of both (CQ), to attenuate adipose inflammation triggered by high fructose (HFr) consumption in rats and by tumor necrosis factor alpha (TNF-α) in 3T3-L1 adipocytes. Methods and results: In rats, HFr consumption for 6 wk caused dyslipidemia, insulin resistance, reduced plasma adiponectin, adiposity, and adipose tissue inflammation. Dietary supplementation with 20 mg/kg/day of C, Q, and CQ improved all these parameters. In 3T3-L1 adipocytes, C and Q attenuated TNF-α-induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP-1, resistin), and decreased adiponectin. The protective effects of C and Q on adipose inflammation are in part associated with their capacity to (i) decrease the activation of the mitogen-activated kinases (MAPKs) JNK and p38; and (ii) prevent the downregulation of PPAR-γ. In summary, C and Q, and to a larger extent the combination of both, attenuated adipose proinflammatory signaling cascades and regulated the balance of molecules that improve (adiponectin) or impair (TNF-α, MCP-1, resistin) insulin sensitivity. Conclusion: Together, these findings suggest that dietary Q and C may have potential benefits in mitigating MetS-associated adipose inflammation, oxidative stress, and insulin resistance.
AB - Scope: This study evaluated the capacity of dietary catechin (C), quercetin (Q), and the combination of both (CQ), to attenuate adipose inflammation triggered by high fructose (HFr) consumption in rats and by tumor necrosis factor alpha (TNF-α) in 3T3-L1 adipocytes. Methods and results: In rats, HFr consumption for 6 wk caused dyslipidemia, insulin resistance, reduced plasma adiponectin, adiposity, and adipose tissue inflammation. Dietary supplementation with 20 mg/kg/day of C, Q, and CQ improved all these parameters. In 3T3-L1 adipocytes, C and Q attenuated TNF-α-induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP-1, resistin), and decreased adiponectin. The protective effects of C and Q on adipose inflammation are in part associated with their capacity to (i) decrease the activation of the mitogen-activated kinases (MAPKs) JNK and p38; and (ii) prevent the downregulation of PPAR-γ. In summary, C and Q, and to a larger extent the combination of both, attenuated adipose proinflammatory signaling cascades and regulated the balance of molecules that improve (adiponectin) or impair (TNF-α, MCP-1, resistin) insulin sensitivity. Conclusion: Together, these findings suggest that dietary Q and C may have potential benefits in mitigating MetS-associated adipose inflammation, oxidative stress, and insulin resistance.
KW - Adipokines
KW - Adipose tissue inflammation
KW - Flavonoids
KW - High fructose diet
KW - Metabolic syndrome
UR - http://www.scopus.com/inward/record.url?scp=84926123215&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84926123215&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201400631
DO - 10.1002/mnfr.201400631
M3 - Article
C2 - 25620282
AN - SCOPUS:84926123215
VL - 59
SP - 622
EP - 633
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
SN - 1613-4125
IS - 4
ER -