Catch and release cell sorting: Electrochemical desorption of T-cells from antibody-modified microelectrodes

He Zhu, Jun Yan, Alexander Revzin

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The development of integrated microsystems capable of interrogation, characterization and sorting of mammalian cells is highly significant for further advancement of point-of-care diagnostics and drug discovery fields. The present study sought to design a novel strategy for releasing antibody-bound cells through electrochemical disruption of the underlying antibody (Ab) layer. A microsystem for selective capture and release of cells consisted of an array of individually addressable gold microelectrodes fabricated on a glass substrate. Poly(ethylene glycol) (PEG) hydrogel photolithography was employed to make the glass regions non-fouling, thus, ensuring selective localization of proteins and cells on the microelectrodes. The gold surfaces were decorated with anti-CD4 Ab molecules using standard alkanethiol self-assembly and carbodiimide coupling approaches. The Ab-functionalized electrodes selectively captured model T-lymphocytes (Molt-3 cells) expressing CD4 antigen while minimal cell adhesion was observed on PEG hydrogel-modified glass substrates. Importantly, application of a reductive potential (-1.2 V vs. Ag/AgCl reference electrode) resulted in release of surface-bound T-cells from the electrode surface. Cyclic voltammetry and fluorescence microscopy were employed to verify that the detachment of captured T-cells was indeed due to the electrochemical disruption of the underlying alkanethiol-Ab layer. In the future, the cell sorting approach described here may be combined with microfluidic delivery to enable Ab-mediated capture of T-lymphocytes or other cell types followed by release of select cells for downstream gene expression studies or re-cultivation.

Original languageEnglish (US)
Pages (from-to)260-268
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume64
Issue number2
DOIs
StatePublished - Jul 15 2008

Fingerprint

T-cells
Microelectrodes
classifying
antibodies
Sorting
Antibodies
Desorption
desorption
Cells
T-Lymphocytes
cells
Polyethylene glycols
Hydrogel
lymphocytes
Microsystems
Glass
Hydrogels
Gold
CD4 Antigens
Electrodes

Keywords

  • Cell micropatterning
  • Cell sorting
  • Leukocyte immunophenotyping
  • Microelectrodes
  • Microfabrication
  • Switchable biointerface

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Catch and release cell sorting : Electrochemical desorption of T-cells from antibody-modified microelectrodes. / Zhu, He; Yan, Jun; Revzin, Alexander.

In: Colloids and Surfaces B: Biointerfaces, Vol. 64, No. 2, 15.07.2008, p. 260-268.

Research output: Contribution to journalArticle

@article{a26150d4c7d0437a9bc2461ac19a325e,
title = "Catch and release cell sorting: Electrochemical desorption of T-cells from antibody-modified microelectrodes",
abstract = "The development of integrated microsystems capable of interrogation, characterization and sorting of mammalian cells is highly significant for further advancement of point-of-care diagnostics and drug discovery fields. The present study sought to design a novel strategy for releasing antibody-bound cells through electrochemical disruption of the underlying antibody (Ab) layer. A microsystem for selective capture and release of cells consisted of an array of individually addressable gold microelectrodes fabricated on a glass substrate. Poly(ethylene glycol) (PEG) hydrogel photolithography was employed to make the glass regions non-fouling, thus, ensuring selective localization of proteins and cells on the microelectrodes. The gold surfaces were decorated with anti-CD4 Ab molecules using standard alkanethiol self-assembly and carbodiimide coupling approaches. The Ab-functionalized electrodes selectively captured model T-lymphocytes (Molt-3 cells) expressing CD4 antigen while minimal cell adhesion was observed on PEG hydrogel-modified glass substrates. Importantly, application of a reductive potential (-1.2 V vs. Ag/AgCl reference electrode) resulted in release of surface-bound T-cells from the electrode surface. Cyclic voltammetry and fluorescence microscopy were employed to verify that the detachment of captured T-cells was indeed due to the electrochemical disruption of the underlying alkanethiol-Ab layer. In the future, the cell sorting approach described here may be combined with microfluidic delivery to enable Ab-mediated capture of T-lymphocytes or other cell types followed by release of select cells for downstream gene expression studies or re-cultivation.",
keywords = "Cell micropatterning, Cell sorting, Leukocyte immunophenotyping, Microelectrodes, Microfabrication, Switchable biointerface",
author = "He Zhu and Jun Yan and Alexander Revzin",
year = "2008",
month = "7",
day = "15",
doi = "10.1016/j.colsurfb.2008.02.010",
language = "English (US)",
volume = "64",
pages = "260--268",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Catch and release cell sorting

T2 - Electrochemical desorption of T-cells from antibody-modified microelectrodes

AU - Zhu, He

AU - Yan, Jun

AU - Revzin, Alexander

PY - 2008/7/15

Y1 - 2008/7/15

N2 - The development of integrated microsystems capable of interrogation, characterization and sorting of mammalian cells is highly significant for further advancement of point-of-care diagnostics and drug discovery fields. The present study sought to design a novel strategy for releasing antibody-bound cells through electrochemical disruption of the underlying antibody (Ab) layer. A microsystem for selective capture and release of cells consisted of an array of individually addressable gold microelectrodes fabricated on a glass substrate. Poly(ethylene glycol) (PEG) hydrogel photolithography was employed to make the glass regions non-fouling, thus, ensuring selective localization of proteins and cells on the microelectrodes. The gold surfaces were decorated with anti-CD4 Ab molecules using standard alkanethiol self-assembly and carbodiimide coupling approaches. The Ab-functionalized electrodes selectively captured model T-lymphocytes (Molt-3 cells) expressing CD4 antigen while minimal cell adhesion was observed on PEG hydrogel-modified glass substrates. Importantly, application of a reductive potential (-1.2 V vs. Ag/AgCl reference electrode) resulted in release of surface-bound T-cells from the electrode surface. Cyclic voltammetry and fluorescence microscopy were employed to verify that the detachment of captured T-cells was indeed due to the electrochemical disruption of the underlying alkanethiol-Ab layer. In the future, the cell sorting approach described here may be combined with microfluidic delivery to enable Ab-mediated capture of T-lymphocytes or other cell types followed by release of select cells for downstream gene expression studies or re-cultivation.

AB - The development of integrated microsystems capable of interrogation, characterization and sorting of mammalian cells is highly significant for further advancement of point-of-care diagnostics and drug discovery fields. The present study sought to design a novel strategy for releasing antibody-bound cells through electrochemical disruption of the underlying antibody (Ab) layer. A microsystem for selective capture and release of cells consisted of an array of individually addressable gold microelectrodes fabricated on a glass substrate. Poly(ethylene glycol) (PEG) hydrogel photolithography was employed to make the glass regions non-fouling, thus, ensuring selective localization of proteins and cells on the microelectrodes. The gold surfaces were decorated with anti-CD4 Ab molecules using standard alkanethiol self-assembly and carbodiimide coupling approaches. The Ab-functionalized electrodes selectively captured model T-lymphocytes (Molt-3 cells) expressing CD4 antigen while minimal cell adhesion was observed on PEG hydrogel-modified glass substrates. Importantly, application of a reductive potential (-1.2 V vs. Ag/AgCl reference electrode) resulted in release of surface-bound T-cells from the electrode surface. Cyclic voltammetry and fluorescence microscopy were employed to verify that the detachment of captured T-cells was indeed due to the electrochemical disruption of the underlying alkanethiol-Ab layer. In the future, the cell sorting approach described here may be combined with microfluidic delivery to enable Ab-mediated capture of T-lymphocytes or other cell types followed by release of select cells for downstream gene expression studies or re-cultivation.

KW - Cell micropatterning

KW - Cell sorting

KW - Leukocyte immunophenotyping

KW - Microelectrodes

KW - Microfabrication

KW - Switchable biointerface

UR - http://www.scopus.com/inward/record.url?scp=43849086332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43849086332&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2008.02.010

DO - 10.1016/j.colsurfb.2008.02.010

M3 - Article

C2 - 18394868

AN - SCOPUS:43849086332

VL - 64

SP - 260

EP - 268

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 2

ER -