Catastrophic chromosomal restructuring during genome elimination in plants

Ek Han Tan; Isabelle M. Henry; Maruthachalam Ravi; Keith R. Bradnam; Terezie Mandakova; Mohan P A Marimuthu; Ian F Korf; Martin A. Lysak; Luca Comai; Simon W L Chan

doi:10.7554/eLife.06516

Catastrophic chromosomal restructuring during genome elimination in plants

Ek Han Tan, Isabelle M. Henry, Maruthachalam Ravi, Keith R. Bradnam, Terezie Mandakova, Mohan P A Marimuthu, Ian F Korf, Martin A. Lysak, Luca Comai, Simon W L Chan

Molecular and Cellular Biology

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.

Original language	English (US)
Article number	e06516
Journal	eLife
Volume	4
Issue number	MAY
DOIs	https://doi.org/10.7554/eLife.06516
State	Published - May 15 2015

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Medicine(all)
Neuroscience(all)

Access to Document

10.7554/eLife.06516

Fingerprint Dive into the research topics of 'Catastrophic chromosomal restructuring during genome elimination in plants'. Together they form a unique fingerprint.

Cite this

@article{61d9ab424a5d4c8387c52f7b05097abe,

title = "Catastrophic chromosomal restructuring during genome elimination in plants",

abstract = "Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.",

author = "Tan, {Ek Han} and Henry, {Isabelle M.} and Maruthachalam Ravi and Bradnam, {Keith R.} and Terezie Mandakova and Marimuthu, {Mohan P A} and Korf, {Ian F} and Lysak, {Martin A.} and Luca Comai and Chan, {Simon W L}",

year = "2015",

month = may,

day = "15",

doi = "10.7554/eLife.06516",

language = "English (US)",

volume = "4",

journal = "eLife",

issn = "2050-084X",

publisher = "eLife Sciences Publications",

number = "MAY",

}

TY - JOUR

T1 - Catastrophic chromosomal restructuring during genome elimination in plants

AU - Tan, Ek Han

AU - Henry, Isabelle M.

AU - Ravi, Maruthachalam

AU - Bradnam, Keith R.

AU - Mandakova, Terezie

AU - Marimuthu, Mohan P A

AU - Korf, Ian F

AU - Lysak, Martin A.

AU - Comai, Luca

AU - Chan, Simon W L

PY - 2015/5/15

Y1 - 2015/5/15

N2 - Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.

AB - Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.

UR - http://www.scopus.com/inward/record.url?scp=84930683882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84930683882&partnerID=8YFLogxK

U2 - 10.7554/eLife.06516

DO - 10.7554/eLife.06516

M3 - Article

C2 - 25977984

AN - SCOPUS:84930683882

VL - 4

JO - eLife

JF - eLife

SN - 2050-084X

IS - MAY

M1 - e06516

ER -

Catastrophic chromosomal restructuring during genome elimination in plants

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this