Abstract
Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.
| Original language | English (US) |
|---|---|
| Article number | e06516 |
| Journal | eLife |
| Volume | 4 |
| Issue number | MAY |
| DOIs | |
| State | Published - May 15 2015 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Medicine(all)
- Neuroscience(all)
Cite this
Catastrophic chromosomal restructuring during genome elimination in plants. / Tan, Ek Han; Henry, Isabelle M.; Ravi, Maruthachalam; Bradnam, Keith R.; Mandakova, Terezie; Marimuthu, Mohan P A; Korf, Ian F; Lysak, Martin A.; Comai, Luca; Chan, Simon W L.
In: eLife, Vol. 4, No. MAY, e06516, 15.05.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Catastrophic chromosomal restructuring during genome elimination in plants
AU - Tan, Ek Han
AU - Henry, Isabelle M.
AU - Ravi, Maruthachalam
AU - Bradnam, Keith R.
AU - Mandakova, Terezie
AU - Marimuthu, Mohan P A
AU - Korf, Ian F
AU - Lysak, Martin A.
AU - Comai, Luca
AU - Chan, Simon W L
PY - 2015/5/15
Y1 - 2015/5/15
N2 - Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.
AB - Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.
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U2 - 10.7554/eLife.06516
DO - 10.7554/eLife.06516
M3 - Article
C2 - 25977984
AN - SCOPUS:84930683882
VL - 4
JO - eLife
JF - eLife
SN - 2050-084X
IS - MAY
M1 - e06516
ER -