Catastrophic chromosomal restructuring during genome elimination in plants

Ek Han Tan, Isabelle M. Henry, Maruthachalam Ravi, Keith R. Bradnam, Terezie Mandakova, Mohan P A Marimuthu, Ian F Korf, Martin A. Lysak, Luca Comai, Simon W L Chan

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.

Original languageEnglish (US)
Article numbere06516
JournaleLife
Volume4
Issue numberMAY
DOIs
StatePublished - May 15 2015

Fingerprint

Haploidy
Chromosomes
Genes
Genome
DNA Ligases
Centromere
Genomic Instability
Repair
Arabidopsis
Histones
Enhanced recovery
Joining
Mutation
Neoplasms
Chromothripsis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Tan, E. H., Henry, I. M., Ravi, M., Bradnam, K. R., Mandakova, T., Marimuthu, M. P. A., ... Chan, S. W. L. (2015). Catastrophic chromosomal restructuring during genome elimination in plants. eLife, 4(MAY), [e06516]. https://doi.org/10.7554/eLife.06516

Catastrophic chromosomal restructuring during genome elimination in plants. / Tan, Ek Han; Henry, Isabelle M.; Ravi, Maruthachalam; Bradnam, Keith R.; Mandakova, Terezie; Marimuthu, Mohan P A; Korf, Ian F; Lysak, Martin A.; Comai, Luca; Chan, Simon W L.

In: eLife, Vol. 4, No. MAY, e06516, 15.05.2015.

Research output: Contribution to journalArticle

Tan, EH, Henry, IM, Ravi, M, Bradnam, KR, Mandakova, T, Marimuthu, MPA, Korf, IF, Lysak, MA, Comai, L & Chan, SWL 2015, 'Catastrophic chromosomal restructuring during genome elimination in plants', eLife, vol. 4, no. MAY, e06516. https://doi.org/10.7554/eLife.06516
Tan EH, Henry IM, Ravi M, Bradnam KR, Mandakova T, Marimuthu MPA et al. Catastrophic chromosomal restructuring during genome elimination in plants. eLife. 2015 May 15;4(MAY). e06516. https://doi.org/10.7554/eLife.06516
Tan, Ek Han ; Henry, Isabelle M. ; Ravi, Maruthachalam ; Bradnam, Keith R. ; Mandakova, Terezie ; Marimuthu, Mohan P A ; Korf, Ian F ; Lysak, Martin A. ; Comai, Luca ; Chan, Simon W L. / Catastrophic chromosomal restructuring during genome elimination in plants. In: eLife. 2015 ; Vol. 4, No. MAY.
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