Catalytic contributions of key residues in the adenine glycosylase muty revealed by pH-dependent kinetics and cellular repair assays

Megan K. Brinkmeyer, Mary Ann Pope, Sheila S. David

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

MutY prevent DNA mutations associated with 8-oxoguanine (OG) by catalyzing the removal of adenines opposite OG. pH dependence of the adenine glycosylase activity establish that Asp 138 of MutY must be deprotonated for maximal activity consistent with its role in stabilizing the oxacarbenium ion transition state in an S N1 mechanism. A cellular OG:A repair assay allowed further validation of the critical role of Asp 138. Conservative substitutions of the catalytic residues Asp 138 and Glu 37 resulted in enzymes with a range of activity that were used to correlate the efficiency of adenine excision with overall OG:A repair and suppression of DNA mutations in vivo. The results show that MutY variations that exhibit reduced mismatch affinity result in more dramatic reductions in cellular OG:A repair than those that only compromise adenine excision catalysis.

Original languageEnglish (US)
Pages (from-to)276-286
Number of pages11
JournalChemistry and Biology
Volume19
Issue number2
DOIs
StatePublished - Feb 24 2012

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Assays
Repair
Adenine
Kinetics
Mutation
DNA
Catalysis
DNA Repair
Substitution reactions
mutY adenine glycosylase
8-hydroxyguanine
Ions
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Molecular Biology
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology

Cite this

Catalytic contributions of key residues in the adenine glycosylase muty revealed by pH-dependent kinetics and cellular repair assays. / Brinkmeyer, Megan K.; Pope, Mary Ann; David, Sheila S.

In: Chemistry and Biology, Vol. 19, No. 2, 24.02.2012, p. 276-286.

Research output: Contribution to journalArticle

Brinkmeyer, Megan K. ; Pope, Mary Ann ; David, Sheila S. / Catalytic contributions of key residues in the adenine glycosylase muty revealed by pH-dependent kinetics and cellular repair assays. In: Chemistry and Biology. 2012 ; Vol. 19, No. 2. pp. 276-286.
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