Catalase activity in equine semen

Barry A. Ball, Curtis G. Gravance, Victor Medina, Julie Baumber, Irwin Liu

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Objective - To characterize the activity of catalase in equine semen. Animals - 15 stallions of known and unknown reproductive history. Procedure - Seminal plasma was collected from raw equine semen by centrifugation, and samples of seminal plasma were frozen prior to assay for catalase activity. Tissue samples (n = 3 stallions) from the bulbourethral gland, prostate gland, vesicular gland, and testis were homogenized, and cauda epididymal fluid was collected for determination of catalase activity. Catalase activity was determined as an enzyme kinetic assay by the disappearance of H2O2 as measured by ultraviolet spectrophotometry. Results - Catalase activity in equine seminal plasma was 989.3 ± 167.8 U/ml (mean ± SEM), and the specific activity of catalase in equine seminal plasma was 98.7 ± 29.2 U/mg of protein. Specific activity of catalase in tissue homogenates was significantly higher in the prostate gland (954 ± 270 U/mg of protein) than in the ampulla (59 ± 5 U/mg of protein), bulbourethral gland (54 ± 11 U/mg of protein), vesicular gland (39 ± 3 U/mg of protein), cauda epididymal fluid (11 ± 3 U/mg protein), or testis (54 ± 6 U/mg of protein). Conclusions and Clinical Relevance - Equine seminal plasma contains a high activity of catalase that is derived primarily from prostatic secretions. Procedures such as semen cryopreservation that remove most seminal plasma from semen may reduce the ability to scavenge H2O2 and thereby increase the susceptibility of spermatozoa to oxidative stress. (Am J Vet Hes 2000;61:1026-1030).

Original languageEnglish (US)
Pages (from-to)1026-1030
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume61
Issue number9
DOIs
StatePublished - Jan 1 2000

Fingerprint

Semen
Catalase
Horses
semen
seminal plasma
catalase
horses
bulbourethral gland
proteins
prostate gland
Bulbourethral Glands
seminal vesicles
Proteins
stallions
testes
Testis
accessory sex glands
Prostate
enzyme kinetics
Ultraviolet Spectrophotometry

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Ball, B. A., Gravance, C. G., Medina, V., Baumber, J., & Liu, I. (2000). Catalase activity in equine semen. American Journal of Veterinary Research, 61(9), 1026-1030. https://doi.org/10.2460/ajvr.2000.61.1026

Catalase activity in equine semen. / Ball, Barry A.; Gravance, Curtis G.; Medina, Victor; Baumber, Julie; Liu, Irwin.

In: American Journal of Veterinary Research, Vol. 61, No. 9, 01.01.2000, p. 1026-1030.

Research output: Contribution to journalArticle

Ball, BA, Gravance, CG, Medina, V, Baumber, J & Liu, I 2000, 'Catalase activity in equine semen', American Journal of Veterinary Research, vol. 61, no. 9, pp. 1026-1030. https://doi.org/10.2460/ajvr.2000.61.1026
Ball, Barry A. ; Gravance, Curtis G. ; Medina, Victor ; Baumber, Julie ; Liu, Irwin. / Catalase activity in equine semen. In: American Journal of Veterinary Research. 2000 ; Vol. 61, No. 9. pp. 1026-1030.
@article{aa5e57549d6849ae83557f88a1d67b15,
title = "Catalase activity in equine semen",
abstract = "Objective - To characterize the activity of catalase in equine semen. Animals - 15 stallions of known and unknown reproductive history. Procedure - Seminal plasma was collected from raw equine semen by centrifugation, and samples of seminal plasma were frozen prior to assay for catalase activity. Tissue samples (n = 3 stallions) from the bulbourethral gland, prostate gland, vesicular gland, and testis were homogenized, and cauda epididymal fluid was collected for determination of catalase activity. Catalase activity was determined as an enzyme kinetic assay by the disappearance of H2O2 as measured by ultraviolet spectrophotometry. Results - Catalase activity in equine seminal plasma was 989.3 ± 167.8 U/ml (mean ± SEM), and the specific activity of catalase in equine seminal plasma was 98.7 ± 29.2 U/mg of protein. Specific activity of catalase in tissue homogenates was significantly higher in the prostate gland (954 ± 270 U/mg of protein) than in the ampulla (59 ± 5 U/mg of protein), bulbourethral gland (54 ± 11 U/mg of protein), vesicular gland (39 ± 3 U/mg of protein), cauda epididymal fluid (11 ± 3 U/mg protein), or testis (54 ± 6 U/mg of protein). Conclusions and Clinical Relevance - Equine seminal plasma contains a high activity of catalase that is derived primarily from prostatic secretions. Procedures such as semen cryopreservation that remove most seminal plasma from semen may reduce the ability to scavenge H2O2 and thereby increase the susceptibility of spermatozoa to oxidative stress. (Am J Vet Hes 2000;61:1026-1030).",
author = "Ball, {Barry A.} and Gravance, {Curtis G.} and Victor Medina and Julie Baumber and Irwin Liu",
year = "2000",
month = "1",
day = "1",
doi = "10.2460/ajvr.2000.61.1026",
language = "English (US)",
volume = "61",
pages = "1026--1030",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "9",

}

TY - JOUR

T1 - Catalase activity in equine semen

AU - Ball, Barry A.

AU - Gravance, Curtis G.

AU - Medina, Victor

AU - Baumber, Julie

AU - Liu, Irwin

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Objective - To characterize the activity of catalase in equine semen. Animals - 15 stallions of known and unknown reproductive history. Procedure - Seminal plasma was collected from raw equine semen by centrifugation, and samples of seminal plasma were frozen prior to assay for catalase activity. Tissue samples (n = 3 stallions) from the bulbourethral gland, prostate gland, vesicular gland, and testis were homogenized, and cauda epididymal fluid was collected for determination of catalase activity. Catalase activity was determined as an enzyme kinetic assay by the disappearance of H2O2 as measured by ultraviolet spectrophotometry. Results - Catalase activity in equine seminal plasma was 989.3 ± 167.8 U/ml (mean ± SEM), and the specific activity of catalase in equine seminal plasma was 98.7 ± 29.2 U/mg of protein. Specific activity of catalase in tissue homogenates was significantly higher in the prostate gland (954 ± 270 U/mg of protein) than in the ampulla (59 ± 5 U/mg of protein), bulbourethral gland (54 ± 11 U/mg of protein), vesicular gland (39 ± 3 U/mg of protein), cauda epididymal fluid (11 ± 3 U/mg protein), or testis (54 ± 6 U/mg of protein). Conclusions and Clinical Relevance - Equine seminal plasma contains a high activity of catalase that is derived primarily from prostatic secretions. Procedures such as semen cryopreservation that remove most seminal plasma from semen may reduce the ability to scavenge H2O2 and thereby increase the susceptibility of spermatozoa to oxidative stress. (Am J Vet Hes 2000;61:1026-1030).

AB - Objective - To characterize the activity of catalase in equine semen. Animals - 15 stallions of known and unknown reproductive history. Procedure - Seminal plasma was collected from raw equine semen by centrifugation, and samples of seminal plasma were frozen prior to assay for catalase activity. Tissue samples (n = 3 stallions) from the bulbourethral gland, prostate gland, vesicular gland, and testis were homogenized, and cauda epididymal fluid was collected for determination of catalase activity. Catalase activity was determined as an enzyme kinetic assay by the disappearance of H2O2 as measured by ultraviolet spectrophotometry. Results - Catalase activity in equine seminal plasma was 989.3 ± 167.8 U/ml (mean ± SEM), and the specific activity of catalase in equine seminal plasma was 98.7 ± 29.2 U/mg of protein. Specific activity of catalase in tissue homogenates was significantly higher in the prostate gland (954 ± 270 U/mg of protein) than in the ampulla (59 ± 5 U/mg of protein), bulbourethral gland (54 ± 11 U/mg of protein), vesicular gland (39 ± 3 U/mg of protein), cauda epididymal fluid (11 ± 3 U/mg protein), or testis (54 ± 6 U/mg of protein). Conclusions and Clinical Relevance - Equine seminal plasma contains a high activity of catalase that is derived primarily from prostatic secretions. Procedures such as semen cryopreservation that remove most seminal plasma from semen may reduce the ability to scavenge H2O2 and thereby increase the susceptibility of spermatozoa to oxidative stress. (Am J Vet Hes 2000;61:1026-1030).

UR - http://www.scopus.com/inward/record.url?scp=0034276640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034276640&partnerID=8YFLogxK

U2 - 10.2460/ajvr.2000.61.1026

DO - 10.2460/ajvr.2000.61.1026

M3 - Article

VL - 61

SP - 1026

EP - 1030

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 9

ER -