Catabolism of epoxy fatty esters by the purified epoxide hydrolase from mouse and human liver

Premjit P. Halarnkar, Roger N. Wixtrom, Marilyn H. Silva, Bruce D. Hammock

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Epoxymethylstearate, 9,10- and 12,13-epoxymethyloleates, and a mixture of isomers of epoxymethylarachidonate and diepoxymethylstearate were synthesized, and their metabolic rates were measured using crude and purified cytosolic epoxide hydrolase. Hepatic epoxide hydrolase was purified from human samples and clofibrate-fed mice by affinity chromatography. The major metabolites under these conditions of all the epoxy fatty esters were their vicinal diols whose structures were confirmed by GC-MS. 12,13-Epoxymethyloleate was metabolized faster than 9,10-epoxymethyloleate and other epoxy fatty esters, but all substrates were turned over rapidly. This rapid turnover suggests that epoxy fatty acids may be endogenous substrates for the cytosolic epoxide hydrolase.

Original languageEnglish (US)
Pages (from-to)226-236
Number of pages11
JournalArchives of Biochemistry and Biophysics
Volume272
Issue number1
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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