Abstract
Epoxymethylstearate, 9,10- and 12,13-epoxymethyloleates, and a mixture of isomers of epoxymethylarachidonate and diepoxymethylstearate were synthesized, and their metabolic rates were measured using crude and purified cytosolic epoxide hydrolase. Hepatic epoxide hydrolase was purified from human samples and clofibrate-fed mice by affinity chromatography. The major metabolites under these conditions of all the epoxy fatty esters were their vicinal diols whose structures were confirmed by GC-MS. 12,13-Epoxymethyloleate was metabolized faster than 9,10-epoxymethyloleate and other epoxy fatty esters, but all substrates were turned over rapidly. This rapid turnover suggests that epoxy fatty acids may be endogenous substrates for the cytosolic epoxide hydrolase.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 226-236 |
| Number of pages | 11 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 272 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1989 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Catabolism of epoxy fatty esters by the purified epoxide hydrolase from mouse and human liver. / Halarnkar, Premjit P.; Wixtrom, Roger N.; Silva, Marilyn H.; Hammock, Bruce D.
In: Archives of Biochemistry and Biophysics, Vol. 272, No. 1, 1989, p. 226-236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Catabolism of epoxy fatty esters by the purified epoxide hydrolase from mouse and human liver
AU - Halarnkar, Premjit P.
AU - Wixtrom, Roger N.
AU - Silva, Marilyn H.
AU - Hammock, Bruce D.
PY - 1989
Y1 - 1989
N2 - Epoxymethylstearate, 9,10- and 12,13-epoxymethyloleates, and a mixture of isomers of epoxymethylarachidonate and diepoxymethylstearate were synthesized, and their metabolic rates were measured using crude and purified cytosolic epoxide hydrolase. Hepatic epoxide hydrolase was purified from human samples and clofibrate-fed mice by affinity chromatography. The major metabolites under these conditions of all the epoxy fatty esters were their vicinal diols whose structures were confirmed by GC-MS. 12,13-Epoxymethyloleate was metabolized faster than 9,10-epoxymethyloleate and other epoxy fatty esters, but all substrates were turned over rapidly. This rapid turnover suggests that epoxy fatty acids may be endogenous substrates for the cytosolic epoxide hydrolase.
AB - Epoxymethylstearate, 9,10- and 12,13-epoxymethyloleates, and a mixture of isomers of epoxymethylarachidonate and diepoxymethylstearate were synthesized, and their metabolic rates were measured using crude and purified cytosolic epoxide hydrolase. Hepatic epoxide hydrolase was purified from human samples and clofibrate-fed mice by affinity chromatography. The major metabolites under these conditions of all the epoxy fatty esters were their vicinal diols whose structures were confirmed by GC-MS. 12,13-Epoxymethyloleate was metabolized faster than 9,10-epoxymethyloleate and other epoxy fatty esters, but all substrates were turned over rapidly. This rapid turnover suggests that epoxy fatty acids may be endogenous substrates for the cytosolic epoxide hydrolase.
UR - http://www.scopus.com/inward/record.url?scp=0024361188&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024361188&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(89)90214-2
DO - 10.1016/0003-9861(89)90214-2
M3 - Article
VL - 272
SP - 226
EP - 236
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 1
ER -