Ca2+/calmodulin binding to PSD-95 mediates homeostatic synaptic scaling down

Dhrubajyoti Chowdhury, Matthew Turner, Tommaso Patriarchi, Anne C. Hergarden, David Anderson, Yonghong Zhang, Junqing Sun, Chao-Yin Chen, James B. Ames, Johannes W Hell

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Postsynaptic density protein-95 (PSD-95) localizes AMPA-type glutamate receptors (AMPARs) to postsynaptic sites of glutamatergic synapses. Its postsynaptic displacement is necessary for loss of AMPARs during homeostatic scaling down of synapses. Here, we demonstrate that upon Ca2+ influx, Ca2+/calmodulin (Ca2+/CaM) binding to the N-terminus of PSD-95 mediates postsynaptic loss of PSD-95 and AMPARs during homeostatic scaling down. Our NMR structural analysis identified E17 within the PSD-95 N-terminus as important for binding to Ca2+/CaM by interacting with R126 on CaM. Mutating E17 to R prevented homeostatic scaling down in primary hippocampal neurons, which is rescued via charge inversion by ectopic expression of CaMR 126E, as determined by analysis of miniature excitatory postsynaptic currents. Accordingly, increased binding of Ca2+/CaM to PSD-95 induced by a chronic increase in Ca2+ influx is a critical molecular event in homeostatic downscaling of glutamatergic synaptic transmission.

Original languageEnglish (US)
JournalEMBO Journal
DOIs
StateAccepted/In press - 2017

Fingerprint

Calmodulin
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
Glutamate Receptors
Synapses
Excitatory Postsynaptic Potentials
Structural analysis
Synaptic Transmission
Neurons
Nuclear magnetic resonance
postsynaptic density proteins

Keywords

  • Calcium
  • Calmodulin
  • Dendritic spines
  • Hippocampus
  • PSD-95

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Chowdhury, D., Turner, M., Patriarchi, T., Hergarden, A. C., Anderson, D., Zhang, Y., ... Hell, J. W. (Accepted/In press). Ca2+/calmodulin binding to PSD-95 mediates homeostatic synaptic scaling down. EMBO Journal. https://doi.org/10.15252/embj.201695829

Ca2+/calmodulin binding to PSD-95 mediates homeostatic synaptic scaling down. / Chowdhury, Dhrubajyoti; Turner, Matthew; Patriarchi, Tommaso; Hergarden, Anne C.; Anderson, David; Zhang, Yonghong; Sun, Junqing; Chen, Chao-Yin; Ames, James B.; Hell, Johannes W.

In: EMBO Journal, 2017.

Research output: Contribution to journalArticle

Chowdhury, D, Turner, M, Patriarchi, T, Hergarden, AC, Anderson, D, Zhang, Y, Sun, J, Chen, C-Y, Ames, JB & Hell, JW 2017, 'Ca2+/calmodulin binding to PSD-95 mediates homeostatic synaptic scaling down', EMBO Journal. https://doi.org/10.15252/embj.201695829
Chowdhury D, Turner M, Patriarchi T, Hergarden AC, Anderson D, Zhang Y et al. Ca2+/calmodulin binding to PSD-95 mediates homeostatic synaptic scaling down. EMBO Journal. 2017. https://doi.org/10.15252/embj.201695829
Chowdhury, Dhrubajyoti ; Turner, Matthew ; Patriarchi, Tommaso ; Hergarden, Anne C. ; Anderson, David ; Zhang, Yonghong ; Sun, Junqing ; Chen, Chao-Yin ; Ames, James B. ; Hell, Johannes W. / Ca2+/calmodulin binding to PSD-95 mediates homeostatic synaptic scaling down. In: EMBO Journal. 2017.
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