Ca2+ entry through conductive pathway modulates receptor-mediated increase in microvessel permeability

P. He, X. Zhang, F. E. Curry

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Abstract

We investigated the relationship between receptor-mediated increases in cytoplasmic Ca2+ concentration ([Ca2+](i)) and increased microvessel permeability. In individually perfused venular microvessels of frog mesentery exposed to 10 μM ATP, [Ca2+](i) increased from 59 ± 7 to 172 ± 21 nM within 1 min and then fell back toward control values. The corresponding peak increase in the hydraulic conductivity (L(p)) of the microvessel wall was 5.7 ± 0.5-fold relative to control. After removal of extracellular Ca2+, there was no significant increase in L(p), and the initial increase in [Ca2+](i) was attenuated but not abolished. Depolarization of the endothelial cell membrane with high-K+ Ringer solution reduced the peak increase in [Ca2+](i) to 106 ± 7 nM and attenuated the increase in L(p), 1.8 ± 0.4- fold. The results conform to the hypothesis that Ca2+ entry into endothelial cells is required for acute increase in venular microvessel permeability by inflammatory agents and that the pathway for Ca2+ entry has the properties of a passive conductance pathway. Similar conclusions were reached in previous experiments in frog microvessels exposed to Ca2+ ionophores and perfusates with no plasma proteins. In venular microvessels of hamster mesentery exposed to ATP and bradykinin, a similar pathway for Ca2+ entry was demonstrated in the present experiments. We did not measure permeability changes in hamster microvessels in this study, but these microvessels respond to histamine and ionophores with a transient increase in permeability to macromolecules similar to that measured in frog microvessels [Am. J. Physiol. 268 (Heart Circ. Physiol. 37): H1982-H1991, 1995].

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume271
Issue number6 40-6
StatePublished - 1996

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Keywords

  • 4-[2-[6-(dioctylamino)-2-naphthalenyl]ethenyl]- 1-(3-sulfopropyl)-pyridinium
  • ATP
  • bradykinin
  • cytoplasmic calcium concentration
  • endothelial
  • hydraulic conductivity
  • individually perfused microvessels
  • membrane depolarization
  • membrane potential

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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