Abstract
Rapid conduction in myelinated axons depends on the generation of specialized subcellular domains to which different sets of ion channels are localized. Here, we describe the identification of Caspr2, a mammalian homolog of Drosophila Neurexin IV (Nrx-IV), and show that this neurexin-like protein and the closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region. We further show that Caspr2 specifically associates with Kv1.1, Kv1.2, and their Kvβ2 subunit. This association involves the C-terminal sequence of Caspr2, which contains a putative PDZ binding site. These results suggest a role for Caspr family members in the local differentiation of the axon into distinct functional subdomains.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1037-1047 |
| Number of pages | 11 |
| Journal | Neuron |
| Volume | 24 |
| Issue number | 4 |
| State | Published - Dec 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
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Caspr2, a new member of the Neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels. / Poliak, Sebastian; Gollan, Leora; Martinez, Ricardo; Custer, Andrew; Einheber, Steven; Salzer, James L.; Trimmer, James; Shrager, Peter; Peles, Elior.
In: Neuron, Vol. 24, No. 4, 12.1999, p. 1037-1047.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Caspr2, a new member of the Neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels
AU - Poliak, Sebastian
AU - Gollan, Leora
AU - Martinez, Ricardo
AU - Custer, Andrew
AU - Einheber, Steven
AU - Salzer, James L.
AU - Trimmer, James
AU - Shrager, Peter
AU - Peles, Elior
PY - 1999/12
Y1 - 1999/12
N2 - Rapid conduction in myelinated axons depends on the generation of specialized subcellular domains to which different sets of ion channels are localized. Here, we describe the identification of Caspr2, a mammalian homolog of Drosophila Neurexin IV (Nrx-IV), and show that this neurexin-like protein and the closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region. We further show that Caspr2 specifically associates with Kv1.1, Kv1.2, and their Kvβ2 subunit. This association involves the C-terminal sequence of Caspr2, which contains a putative PDZ binding site. These results suggest a role for Caspr family members in the local differentiation of the axon into distinct functional subdomains.
AB - Rapid conduction in myelinated axons depends on the generation of specialized subcellular domains to which different sets of ion channels are localized. Here, we describe the identification of Caspr2, a mammalian homolog of Drosophila Neurexin IV (Nrx-IV), and show that this neurexin-like protein and the closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region. We further show that Caspr2 specifically associates with Kv1.1, Kv1.2, and their Kvβ2 subunit. This association involves the C-terminal sequence of Caspr2, which contains a putative PDZ binding site. These results suggest a role for Caspr family members in the local differentiation of the axon into distinct functional subdomains.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033396331&partnerID=8YFLogxK
M3 - Article
VL - 24
SP - 1037
EP - 1047
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 4
ER -