Caspr2, a new member of the Neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels

Sebastian Poliak, Leora Gollan, Ricardo Martinez, Andrew Custer, Steven Einheber, James L. Salzer, James Trimmer, Peter Shrager, Elior Peles

Research output: Contribution to journalArticle

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Abstract

Rapid conduction in myelinated axons depends on the generation of specialized subcellular domains to which different sets of ion channels are localized. Here, we describe the identification of Caspr2, a mammalian homolog of Drosophila Neurexin IV (Nrx-IV), and show that this neurexin-like protein and the closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region. We further show that Caspr2 specifically associates with Kv1.1, Kv1.2, and their Kvβ2 subunit. This association involves the C-terminal sequence of Caspr2, which contains a putative PDZ binding site. These results suggest a role for Caspr family members in the local differentiation of the axon into distinct functional subdomains.

Original languageEnglish (US)
Pages (from-to)1037-1047
Number of pages11
JournalNeuron
Volume24
Issue number4
StatePublished - Dec 1999
Externally publishedYes

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Contactins
Axons
Proteins
Ion Channels
Drosophila
Binding Sites

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Poliak, S., Gollan, L., Martinez, R., Custer, A., Einheber, S., Salzer, J. L., ... Peles, E. (1999). Caspr2, a new member of the Neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels. Neuron, 24(4), 1037-1047.

Caspr2, a new member of the Neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels. / Poliak, Sebastian; Gollan, Leora; Martinez, Ricardo; Custer, Andrew; Einheber, Steven; Salzer, James L.; Trimmer, James; Shrager, Peter; Peles, Elior.

In: Neuron, Vol. 24, No. 4, 12.1999, p. 1037-1047.

Research output: Contribution to journalArticle

Poliak, S, Gollan, L, Martinez, R, Custer, A, Einheber, S, Salzer, JL, Trimmer, J, Shrager, P & Peles, E 1999, 'Caspr2, a new member of the Neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels', Neuron, vol. 24, no. 4, pp. 1037-1047.
Poliak S, Gollan L, Martinez R, Custer A, Einheber S, Salzer JL et al. Caspr2, a new member of the Neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels. Neuron. 1999 Dec;24(4):1037-1047.
Poliak, Sebastian ; Gollan, Leora ; Martinez, Ricardo ; Custer, Andrew ; Einheber, Steven ; Salzer, James L. ; Trimmer, James ; Shrager, Peter ; Peles, Elior. / Caspr2, a new member of the Neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels. In: Neuron. 1999 ; Vol. 24, No. 4. pp. 1037-1047.
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