Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo

Francisco R Carvallo chaigneau, Cristina Trejo-Solís, Celedonio Gómez-Ruiz, Ernesto Rodríguez-Aguilera, Lucía MacÍas-Rosales, Edith Cortés-Barberena, Carlos Cedillo-Peláez, Isabel Gracia-Mora, Lena Ruiz-Azuara, Vicente Madrid-Marina, Fernando Constantino-Casas

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4′-dimethyl-2,2′- bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.

Original languageEnglish (US)
Pages (from-to)17-28
Number of pages12
JournalBioMetals
Volume21
Issue number1
DOIs
StatePublished - Feb 2008
Externally publishedYes

Keywords

  • Apoptosis
  • Bax
  • Casiopeina
  • Chemotherapy
  • p53(-/-)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Carvallo chaigneau, F. R., Trejo-Solís, C., Gómez-Ruiz, C., Rodríguez-Aguilera, E., MacÍas-Rosales, L., Cortés-Barberena, E., Cedillo-Peláez, C., Gracia-Mora, I., Ruiz-Azuara, L., Madrid-Marina, V., & Constantino-Casas, F. (2008). Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo. BioMetals, 21(1), 17-28. https://doi.org/10.1007/s10534-007-9089-4