Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo

Francisco R Carvallo chaigneau, Cristina Trejo-Solís, Celedonio Gómez-Ruiz, Ernesto Rodríguez-Aguilera, Lucía MacÍas-Rosales, Edith Cortés-Barberena, Carlos Cedillo-Peláez, Isabel Gracia-Mora, Lena Ruiz-Azuara, Vicente Madrid-Marina, Fernando Constantino-Casas

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4′-dimethyl-2,2′- bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.

Original languageEnglish (US)
Pages (from-to)17-28
Number of pages12
JournalBioMetals
Volume21
Issue number1
DOIs
StatePublished - Feb 2008
Externally publishedYes

Fingerprint

Chemotherapy
caspases
Cell death
Caspases
colon
Tumors
Colon
apoptosis
Apoptosis
drug therapy
Refractory materials
Drug Therapy
Nitrates
neoplasms
DNA
antineoplastic agents
DNA fragmentation
Cells
DNA Fragmentation
cells

Keywords

  • Apoptosis
  • Bax
  • Casiopeina
  • Chemotherapy
  • p53(-/-)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Carvallo chaigneau, F. R., Trejo-Solís, C., Gómez-Ruiz, C., Rodríguez-Aguilera, E., MacÍas-Rosales, L., Cortés-Barberena, E., ... Constantino-Casas, F. (2008). Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo. BioMetals, 21(1), 17-28. https://doi.org/10.1007/s10534-007-9089-4

Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo. / Carvallo chaigneau, Francisco R; Trejo-Solís, Cristina; Gómez-Ruiz, Celedonio; Rodríguez-Aguilera, Ernesto; MacÍas-Rosales, Lucía; Cortés-Barberena, Edith; Cedillo-Peláez, Carlos; Gracia-Mora, Isabel; Ruiz-Azuara, Lena; Madrid-Marina, Vicente; Constantino-Casas, Fernando.

In: BioMetals, Vol. 21, No. 1, 02.2008, p. 17-28.

Research output: Contribution to journalArticle

Carvallo chaigneau, FR, Trejo-Solís, C, Gómez-Ruiz, C, Rodríguez-Aguilera, E, MacÍas-Rosales, L, Cortés-Barberena, E, Cedillo-Peláez, C, Gracia-Mora, I, Ruiz-Azuara, L, Madrid-Marina, V & Constantino-Casas, F 2008, 'Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo', BioMetals, vol. 21, no. 1, pp. 17-28. https://doi.org/10.1007/s10534-007-9089-4
Carvallo chaigneau, Francisco R ; Trejo-Solís, Cristina ; Gómez-Ruiz, Celedonio ; Rodríguez-Aguilera, Ernesto ; MacÍas-Rosales, Lucía ; Cortés-Barberena, Edith ; Cedillo-Peláez, Carlos ; Gracia-Mora, Isabel ; Ruiz-Azuara, Lena ; Madrid-Marina, Vicente ; Constantino-Casas, Fernando. / Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo. In: BioMetals. 2008 ; Vol. 21, No. 1. pp. 17-28.
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