Abstract
The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4′-dimethyl-2,2′- bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17-28 |
| Number of pages | 12 |
| Journal | BioMetals |
| Volume | 21 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2008 |
| Externally published | Yes |
Fingerprint
Keywords
- Apoptosis
- Bax
- Casiopeina
- Chemotherapy
- p53(-/-)
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo. / Carvallo chaigneau, Francisco R; Trejo-Solís, Cristina; Gómez-Ruiz, Celedonio; Rodríguez-Aguilera, Ernesto; MacÍas-Rosales, Lucía; Cortés-Barberena, Edith; Cedillo-Peláez, Carlos; Gracia-Mora, Isabel; Ruiz-Azuara, Lena; Madrid-Marina, Vicente; Constantino-Casas, Fernando.
In: BioMetals, Vol. 21, No. 1, 02.2008, p. 17-28.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo
AU - Carvallo chaigneau, Francisco R
AU - Trejo-Solís, Cristina
AU - Gómez-Ruiz, Celedonio
AU - Rodríguez-Aguilera, Ernesto
AU - MacÍas-Rosales, Lucía
AU - Cortés-Barberena, Edith
AU - Cedillo-Peláez, Carlos
AU - Gracia-Mora, Isabel
AU - Ruiz-Azuara, Lena
AU - Madrid-Marina, Vicente
AU - Constantino-Casas, Fernando
PY - 2008/2
Y1 - 2008/2
N2 - The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4′-dimethyl-2,2′- bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.
AB - The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4′-dimethyl-2,2′- bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.
KW - Apoptosis
KW - Bax
KW - Casiopeina
KW - Chemotherapy
KW - p53(-/-)
UR - http://www.scopus.com/inward/record.url?scp=38649143668&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38649143668&partnerID=8YFLogxK
U2 - 10.1007/s10534-007-9089-4
DO - 10.1007/s10534-007-9089-4
M3 - Article
C2 - 17390215
AN - SCOPUS:38649143668
VL - 21
SP - 17
EP - 28
JO - BioMetals
JF - BioMetals
SN - 0966-0844
IS - 1
ER -