Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo

Francisco R Carvallo chaigneau; Cristina Trejo-Solís; Celedonio Gómez-Ruiz; Ernesto Rodríguez-Aguilera; Lucía MacÍas-Rosales; Edith Cortés-Barberena; Carlos Cedillo-Peláez; Isabel Gracia-Mora; Lena Ruiz-Azuara; Vicente Madrid-Marina; Fernando Constantino-Casas

doi:10.1007/s10534-007-9089-4

Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo

Francisco R Carvallo chaigneau, Cristina Trejo-Solís, Celedonio Gómez-Ruiz, Ernesto Rodríguez-Aguilera, Lucía MacÍas-Rosales, Edith Cortés-Barberena, Carlos Cedillo-Peláez, Isabel Gracia-Mora, Lena Ruiz-Azuara, Vicente Madrid-Marina, Fernando Constantino-Casas

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4′-dimethyl-2,2′- bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.

Original language	English (US)
Pages (from-to)	17-28
Number of pages	12
Journal	BioMetals
Volume	21
Issue number	1
DOIs	https://doi.org/10.1007/s10534-007-9089-4
State	Published - Feb 2008
Externally published	Yes

Keywords

Apoptosis
Bax
Casiopeina
Chemotherapy
p53(-/-)

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)

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10.1007/s10534-007-9089-4

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Carvallo chaigneau, F. R., Trejo-Solís, C., Gómez-Ruiz, C., Rodríguez-Aguilera, E., MacÍas-Rosales, L., Cortés-Barberena, E., Cedillo-Peláez, C., Gracia-Mora, I., Ruiz-Azuara, L., Madrid-Marina, V., & Constantino-Casas, F. (2008). Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo. BioMetals, 21(1), 17-28. https://doi.org/10.1007/s10534-007-9089-4

Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo. / Carvallo chaigneau, Francisco R; Trejo-Solís, Cristina; Gómez-Ruiz, Celedonio; Rodríguez-Aguilera, Ernesto; MacÍas-Rosales, Lucía; Cortés-Barberena, Edith; Cedillo-Peláez, Carlos; Gracia-Mora, Isabel; Ruiz-Azuara, Lena; Madrid-Marina, Vicente; Constantino-Casas, Fernando.

In: BioMetals, Vol. 21, No. 1, 02.2008, p. 17-28.

Research output: Contribution to journal › Article › peer-review

Carvallo chaigneau, FR, Trejo-Solís, C, Gómez-Ruiz, C, Rodríguez-Aguilera, E, MacÍas-Rosales, L, Cortés-Barberena, E, Cedillo-Peláez, C, Gracia-Mora, I, Ruiz-Azuara, L, Madrid-Marina, V & Constantino-Casas, F 2008, 'Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo', BioMetals, vol. 21, no. 1, pp. 17-28. https://doi.org/10.1007/s10534-007-9089-4

Carvallo chaigneau, Francisco R ; Trejo-Solís, Cristina ; Gómez-Ruiz, Celedonio ; Rodríguez-Aguilera, Ernesto ; MacÍas-Rosales, Lucía ; Cortés-Barberena, Edith ; Cedillo-Peláez, Carlos ; Gracia-Mora, Isabel ; Ruiz-Azuara, Lena ; Madrid-Marina, Vicente ; Constantino-Casas, Fernando. / Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo. In: BioMetals. 2008 ; Vol. 21, No. 1. pp. 17-28.

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abstract = "The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4′-dimethyl-2,2′- bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.",

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Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo

Abstract

Keywords

ASJC Scopus subject areas

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