Cartilage-derived morphogenetic proteins: New members of the transforming growth factor-β superfamily predominantly expressed in long bones during human embryonic development

Steven C. Chang, Bang Hoang, J. Terrig Thomas, Slobodan Vukicevic, Frank P. Luyten, Nicholas J P Ryba, Christine A. Kozak, A Hari Reddi, Malcolm Moos

Research output: Contribution to journalArticle

342 Citations (Scopus)

Abstract

Partially purified extracts from newborn calf articular cartilage were found to induce cartilage and bone when subcutaneously implanted in rats. This activity showed characteristics of bone morphogenetic proteins (BMPs). Degenerate oligonucleotide primer sets derived from the highly conserved carboxyl-terminal region of the BMP family were designed and used in reverse transcription-polymerase chain reactions with poly(A)+ RNA from articular cartilage as template to determine which BMPs are produced by chondrocytes. Two novel members of the transforming growth factor-β (TGF-β) superfamily were identified and designated cartilage-derived morphogenetic protein-1 (CDMP-1) and -2 (CDMP-2). Their carboxyl-terminal TGF-β domains are 82% identical, thus defining a novel subfamily most closely related to BMP-5, BMP-6, and osteogenic protein-1. Northern analyses showed that both genes are predominantly expressed in cartilaginous tissues. In situ hybridization and immunostaining of sections from human embryos showed that CDMP-1 was predominantly found at the stage of precartilaginous mesenchymal condensation and throughout the cartilaginous cores of the developing long bones, whereas CDMP-2 expression was restricted to the hypertrophic chondrocytes of ossifying long bone centers. Neither gene was detectable in the axial skeleton during human embryonic development. The cartilage-specific localization pattern of these novel TGF-β superfamily members, which contrasts with the more ubiquitous presence of other BMP family members, suggests a potential role for these proteins in chondrocyte differentiation and growth of long bones.

Original languageEnglish (US)
Pages (from-to)28227-28234
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number45
StatePublished - Nov 11 1994
Externally publishedYes

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Bone Morphogenetic Proteins
Growth Differentiation Factor 5
Cartilage
Human Development
Transforming Growth Factors
Embryonic Development
Bone
Chondrocytes
Bone and Bones
Articular Cartilage
Bone Morphogenetic Protein 5
Proteins
Bone Morphogenetic Protein 6
Genes
Bone Morphogenetic Protein 7
DNA Primers
Polymerase chain reaction
Bone Development
Transcription
Skeleton

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cartilage-derived morphogenetic proteins : New members of the transforming growth factor-β superfamily predominantly expressed in long bones during human embryonic development. / Chang, Steven C.; Hoang, Bang; Thomas, J. Terrig; Vukicevic, Slobodan; Luyten, Frank P.; Ryba, Nicholas J P; Kozak, Christine A.; Reddi, A Hari; Moos, Malcolm.

In: Journal of Biological Chemistry, Vol. 269, No. 45, 11.11.1994, p. 28227-28234.

Research output: Contribution to journalArticle

Chang, Steven C. ; Hoang, Bang ; Thomas, J. Terrig ; Vukicevic, Slobodan ; Luyten, Frank P. ; Ryba, Nicholas J P ; Kozak, Christine A. ; Reddi, A Hari ; Moos, Malcolm. / Cartilage-derived morphogenetic proteins : New members of the transforming growth factor-β superfamily predominantly expressed in long bones during human embryonic development. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 45. pp. 28227-28234.
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