Carnitine palmitoyltransferase 1C regulates cancer cell senescence through mitochondria-associated metabolic reprograming

Yongtao Wang, Yixin Chen, Lihuan Guan, Huizheng Zhang, Yaoyao Huang, Caroline H. Johnson, Zeming Wu, Frank J. Gonzalez, Aiming Yu, Peng Huang, Ying Wang, Shouhui Yang, Pan Chen, Xiaomei Fan, Min Huang, Huichang Bi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cellular senescence is a fundamental biological process that has profound implications in cancer development and therapeutics, but the underlying mechanisms remain elusive. Here we show that carnitine palmitoyltransferase 1C (CPT1C), an enzyme that catalyzes carnitinylation of fatty acids for transport into mitochondria for β-oxidation, plays a major role in the regulation of cancer cell senescence through mitochondria-associated metabolic reprograming. Metabolomics analysis suggested alterations in mitochondria activity, as revealed by the marked decrease in acylcarnitines in senescent human pancreatic carcinoma PANC-1 cells, indicating low CPT1C activity. Direct analyses of mRNA and protein show that CPT1C is significantly reduced in senescent cells. Furthermore, abnormal mitochondrial function was observed in senescent PANC-1 cells, leading to lower cell survival under metabolic stress and suppressed tumorigenesis in a mouse xenograft model. Knock-down of CPT1C in PANC-1 cells induced mitochondrial dysfunction, caused senescence-like growth suppression and cellular senescence, suppressed cell survival under metabolic stress, and inhibited tumorigenesis in vivo. Further, CPT1C knock-down suppressed xenograft tumor growth in situ. Silencing of CPT1C in five other tumor cell lines also caused cellular senescence. On the contrary, gain-of-function of CPT1C reversed PANC-1 cell senescence and enhanced mitochondrial function. This study identifies CPT1C as a novel biomarker and key regulator of cancer cell senescence through mitochondria-associated metabolic reprograming, and suggests that inhibition of CPT1C may represent a new therapeutic strategy for cancer treatment through induction of tumor senescence.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalCell Death and Differentiation
DOIs
StateAccepted/In press - Jan 9 2018

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Carnitine O-Palmitoyltransferase
Cell Aging
Mitochondria
Neoplasms
Physiological Stress
Heterografts
Cell Survival
Carcinogenesis
Biological Phenomena
Metabolomics
Growth
Tumor Cell Line
Fatty Acids
Biomarkers
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Carnitine palmitoyltransferase 1C regulates cancer cell senescence through mitochondria-associated metabolic reprograming. / Wang, Yongtao; Chen, Yixin; Guan, Lihuan; Zhang, Huizheng; Huang, Yaoyao; Johnson, Caroline H.; Wu, Zeming; Gonzalez, Frank J.; Yu, Aiming; Huang, Peng; Wang, Ying; Yang, Shouhui; Chen, Pan; Fan, Xiaomei; Huang, Min; Bi, Huichang.

In: Cell Death and Differentiation, 09.01.2018, p. 1-14.

Research output: Contribution to journalArticle

Wang, Y, Chen, Y, Guan, L, Zhang, H, Huang, Y, Johnson, CH, Wu, Z, Gonzalez, FJ, Yu, A, Huang, P, Wang, Y, Yang, S, Chen, P, Fan, X, Huang, M & Bi, H 2018, 'Carnitine palmitoyltransferase 1C regulates cancer cell senescence through mitochondria-associated metabolic reprograming', Cell Death and Differentiation, pp. 1-14. https://doi.org/10.1038/s41418-017-0013-3
Wang, Yongtao ; Chen, Yixin ; Guan, Lihuan ; Zhang, Huizheng ; Huang, Yaoyao ; Johnson, Caroline H. ; Wu, Zeming ; Gonzalez, Frank J. ; Yu, Aiming ; Huang, Peng ; Wang, Ying ; Yang, Shouhui ; Chen, Pan ; Fan, Xiaomei ; Huang, Min ; Bi, Huichang. / Carnitine palmitoyltransferase 1C regulates cancer cell senescence through mitochondria-associated metabolic reprograming. In: Cell Death and Differentiation. 2018 ; pp. 1-14.
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AU - Johnson, Caroline H.

AU - Wu, Zeming

AU - Gonzalez, Frank J.

AU - Yu, Aiming

AU - Huang, Peng

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AU - Yang, Shouhui

AU - Chen, Pan

AU - Fan, Xiaomei

AU - Huang, Min

AU - Bi, Huichang

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AB - Cellular senescence is a fundamental biological process that has profound implications in cancer development and therapeutics, but the underlying mechanisms remain elusive. Here we show that carnitine palmitoyltransferase 1C (CPT1C), an enzyme that catalyzes carnitinylation of fatty acids for transport into mitochondria for β-oxidation, plays a major role in the regulation of cancer cell senescence through mitochondria-associated metabolic reprograming. Metabolomics analysis suggested alterations in mitochondria activity, as revealed by the marked decrease in acylcarnitines in senescent human pancreatic carcinoma PANC-1 cells, indicating low CPT1C activity. Direct analyses of mRNA and protein show that CPT1C is significantly reduced in senescent cells. Furthermore, abnormal mitochondrial function was observed in senescent PANC-1 cells, leading to lower cell survival under metabolic stress and suppressed tumorigenesis in a mouse xenograft model. Knock-down of CPT1C in PANC-1 cells induced mitochondrial dysfunction, caused senescence-like growth suppression and cellular senescence, suppressed cell survival under metabolic stress, and inhibited tumorigenesis in vivo. Further, CPT1C knock-down suppressed xenograft tumor growth in situ. Silencing of CPT1C in five other tumor cell lines also caused cellular senescence. On the contrary, gain-of-function of CPT1C reversed PANC-1 cell senescence and enhanced mitochondrial function. This study identifies CPT1C as a novel biomarker and key regulator of cancer cell senescence through mitochondria-associated metabolic reprograming, and suggests that inhibition of CPT1C may represent a new therapeutic strategy for cancer treatment through induction of tumor senescence.

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