Cardioplegia, Na, and Ca in ischemic newborn myocardium

S. Anderson, A. Beyschau, Hong Liu, Peter M Cala

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The hypothesis that Na-dependent increases in Cai are associated with injury was tested in newborn hearts ± cold crystalloid cardioplegia (CCC). 31P, 23Na and 19F NMR were used to measure intracellular pHi, ATP, Nai, and Cai in 4-7 day old rabbit hearts Langendorff-perfused with 37°C Krebs-Henseleit. Control was 30′ baseline, 40′ ischemia and 40′ reperfusion. CCC was identical except hearts were cooled to 12°C during ischemia after a 5′ infusion of ice cold control perfusate (nKCCC) or high K perfusate (hKCCC; added 11 mM KCl substituted for 11 mM NaCl). Injury was assessed from total CK released during reperfusion. Data are mean±SEM, *p<0.05 vs. control. Group (n) Baseline End Ischemia 25′ Reperfusion pHi control (8) 7.10±0.01 5.83±0.05 7.16±0.03 nKCCC (5) 7.11±0.01 7.07±0.12* 7.22±0.06 hKCCC (3) 7.16±0.07 6.89±0.13* 7.25±0.06 Cai(nM/L) control (3) 310±42 543±38 321±36 nKCCC (3) 261±30 376±55 204±7* hKCCC (3) 200±12 453±107 196±11* Nai control (8) 16±3 109±10 65±10 (mEq/kgdry) nKCCC (4) 27±3 54±4* 15±7* hKCCC(3) 19±1 43±13* 39±4 ATP control (8) 101±2 19±3 34±4 (% Control) nKCCC (4) 100±8 53±8* 73±14* hKCCC (3) 96±4 65±2* 97±17* CK release was 817±153 IU/g dry in control; 297±74* in nKCCC, and 177±43* in hKCCC. Thus, consistent with the hypothesis, after CCC decreased Nai during ischemia was associated with decreased [Ca]i and decreased release of CK during reperfusion.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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