Cardiac stem cell hybrids enhance myocardial repair

Pearl Quijada, Hazel T. Salunga, Nirmala Hariharan, Jonathan D. Cubillo, Farid G. El-Sayed, Maryam Moshref, Kristin M. Bala, Jacqueline M. Emathinger, Andrea De La Torre, Lucia Ormachea, Roberto Alvarez, Natalie A. Gude, Mark A. Sussman

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Rationale: Dual cell transplantation of cardiac progenitor cells (CPCs) and mesenchymal stem cells (MSCs) after infarction improves myocardial repair and performance in large animal models relative to delivery of either cell population. Objective: To demonstrate that CardioChimeras (CCs) formed by fusion between CPCs and MSCs have enhanced reparative potential in a mouse model of myocardial infarction relative to individual stem cells or combined cell delivery. Methods and Results: Two distinct and clonally derived CCs, CC1 and CC2, were used for this study. CCs improved left ventricular anterior wall thickness at 4 weeks post injury, but only CC1 treatment preserved anterior wall thickness at 18 weeks. Ejection fraction was enhanced at 6 weeks in CCs, and functional improvements were maintained in CCs and CPC+MSC groups at 18 weeks. Infarct size was decreased in CCs, whereas CPC+MSC and CPC parent groups remained unchanged at 12 weeks. CCs exhibited increased persistence, engraftment, and expression of early commitment markers within the border zone relative to combinatorial and individual cell population-injected groups. CCs increased capillary density and preserved cardiomyocyte size in the infarcted regions suggesting CCs role in protective paracrine secretion. Conclusions: CCs merge the application of distinct cells into a single entity for cellular therapeutic intervention in the progression of heart failure. CCs are a novel cell therapy that improves on combinatorial cell approaches to support myocardial regeneration.

Original languageEnglish (US)
Pages (from-to)695-706
Number of pages12
JournalCirculation Research
Volume117
Issue number8
DOIs
StatePublished - Sep 25 2015

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Stem Cells
Mesenchymal Stromal Cells
Myocardial Infarction
Cell Transplantation
Cell- and Tissue-Based Therapy
Cardiac Myocytes
Regeneration
Animal Models
Heart Failure
Wounds and Injuries
Therapeutics
Population

Keywords

  • Cell fusion
  • Mesenchymal stromal cells
  • Myocardial infarction
  • Myocytes, cardiac
  • Stem cells

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Quijada, P., Salunga, H. T., Hariharan, N., Cubillo, J. D., El-Sayed, F. G., Moshref, M., ... Sussman, M. A. (2015). Cardiac stem cell hybrids enhance myocardial repair. Circulation Research, 117(8), 695-706. https://doi.org/10.1161/CIRCRESAHA.115.306838

Cardiac stem cell hybrids enhance myocardial repair. / Quijada, Pearl; Salunga, Hazel T.; Hariharan, Nirmala; Cubillo, Jonathan D.; El-Sayed, Farid G.; Moshref, Maryam; Bala, Kristin M.; Emathinger, Jacqueline M.; De La Torre, Andrea; Ormachea, Lucia; Alvarez, Roberto; Gude, Natalie A.; Sussman, Mark A.

In: Circulation Research, Vol. 117, No. 8, 25.09.2015, p. 695-706.

Research output: Contribution to journalArticle

Quijada, P, Salunga, HT, Hariharan, N, Cubillo, JD, El-Sayed, FG, Moshref, M, Bala, KM, Emathinger, JM, De La Torre, A, Ormachea, L, Alvarez, R, Gude, NA & Sussman, MA 2015, 'Cardiac stem cell hybrids enhance myocardial repair', Circulation Research, vol. 117, no. 8, pp. 695-706. https://doi.org/10.1161/CIRCRESAHA.115.306838
Quijada P, Salunga HT, Hariharan N, Cubillo JD, El-Sayed FG, Moshref M et al. Cardiac stem cell hybrids enhance myocardial repair. Circulation Research. 2015 Sep 25;117(8):695-706. https://doi.org/10.1161/CIRCRESAHA.115.306838
Quijada, Pearl ; Salunga, Hazel T. ; Hariharan, Nirmala ; Cubillo, Jonathan D. ; El-Sayed, Farid G. ; Moshref, Maryam ; Bala, Kristin M. ; Emathinger, Jacqueline M. ; De La Torre, Andrea ; Ormachea, Lucia ; Alvarez, Roberto ; Gude, Natalie A. ; Sussman, Mark A. / Cardiac stem cell hybrids enhance myocardial repair. In: Circulation Research. 2015 ; Vol. 117, No. 8. pp. 695-706.
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