Abstract
Exosomes are cell-derived small extracellular membrane vesicles (50–100 nm in diameter) actively secreted by a number of healthy and diseased cell types. Exosomes can mediate cellular, tissue, and organ level micro communication under normal and pathological conditions by shuttling proteins, mRNA, and microRNAs. Prior to vesicle molecular profiling, these exosomes can be isolated from conditioned cell media or bodily fluids such as urine and plasma in order to explore the contents and functional relevance. Exosome purification and analyses are a fast-growing research field. Regardless of several advances in exosome purification and analyses methods, research still faces several challenges. Despite tremendous interest in the role of extracellular vesicles, there is no general agreement on dependable isolation protocols. Therefore, there is an urgent need to establish reliable protocol of exosome purification and analysis. Here, we report a simple cost-effective isolation and analysis of cardiac myocyte exosomes from conditioned media.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 237-248 |
| Number of pages | 12 |
| Volume | 1448 |
| DOIs | |
| State | Published - 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1448 |
| ISSN (Print) | 10643745 |
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Keywords
- Acetyl choline esterase
- Cardiac myocytes
- Cell signaling
- Exosomes
- Heart
- Protocol
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Cardiac myocyte exosome isolation. / Malik, Zulfiqar A.; Liu, Tingting T.; Knowlton, Anne A.
Methods in Molecular Biology. Vol. 1448 Humana Press Inc., 2016. p. 237-248 (Methods in Molecular Biology; Vol. 1448).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Cardiac myocyte exosome isolation
AU - Malik, Zulfiqar A.
AU - Liu, Tingting T.
AU - Knowlton, Anne A.
PY - 2016
Y1 - 2016
N2 - Exosomes are cell-derived small extracellular membrane vesicles (50–100 nm in diameter) actively secreted by a number of healthy and diseased cell types. Exosomes can mediate cellular, tissue, and organ level micro communication under normal and pathological conditions by shuttling proteins, mRNA, and microRNAs. Prior to vesicle molecular profiling, these exosomes can be isolated from conditioned cell media or bodily fluids such as urine and plasma in order to explore the contents and functional relevance. Exosome purification and analyses are a fast-growing research field. Regardless of several advances in exosome purification and analyses methods, research still faces several challenges. Despite tremendous interest in the role of extracellular vesicles, there is no general agreement on dependable isolation protocols. Therefore, there is an urgent need to establish reliable protocol of exosome purification and analysis. Here, we report a simple cost-effective isolation and analysis of cardiac myocyte exosomes from conditioned media.
AB - Exosomes are cell-derived small extracellular membrane vesicles (50–100 nm in diameter) actively secreted by a number of healthy and diseased cell types. Exosomes can mediate cellular, tissue, and organ level micro communication under normal and pathological conditions by shuttling proteins, mRNA, and microRNAs. Prior to vesicle molecular profiling, these exosomes can be isolated from conditioned cell media or bodily fluids such as urine and plasma in order to explore the contents and functional relevance. Exosome purification and analyses are a fast-growing research field. Regardless of several advances in exosome purification and analyses methods, research still faces several challenges. Despite tremendous interest in the role of extracellular vesicles, there is no general agreement on dependable isolation protocols. Therefore, there is an urgent need to establish reliable protocol of exosome purification and analysis. Here, we report a simple cost-effective isolation and analysis of cardiac myocyte exosomes from conditioned media.
KW - Acetyl choline esterase
KW - Cardiac myocytes
KW - Cell signaling
KW - Exosomes
KW - Heart
KW - Protocol
UR - http://www.scopus.com/inward/record.url?scp=84979225470&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84979225470&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3753-0_17
DO - 10.1007/978-1-4939-3753-0_17
M3 - Chapter
C2 - 27317185
AN - SCOPUS:84979225470
VL - 1448
T3 - Methods in Molecular Biology
SP - 237
EP - 248
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -