Cardiac myocyte exosome isolation

Zulfiqar A. Malik, Tingting T. Liu, Anne A. Knowlton

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Exosomes are cell-derived small extracellular membrane vesicles (50–100 nm in diameter) actively secreted by a number of healthy and diseased cell types. Exosomes can mediate cellular, tissue, and organ level micro communication under normal and pathological conditions by shuttling proteins, mRNA, and microRNAs. Prior to vesicle molecular profiling, these exosomes can be isolated from conditioned cell media or bodily fluids such as urine and plasma in order to explore the contents and functional relevance. Exosome purification and analyses are a fast-growing research field. Regardless of several advances in exosome purification and analyses methods, research still faces several challenges. Despite tremendous interest in the role of extracellular vesicles, there is no general agreement on dependable isolation protocols. Therefore, there is an urgent need to establish reliable protocol of exosome purification and analysis. Here, we report a simple cost-effective isolation and analysis of cardiac myocyte exosomes from conditioned media.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages237-248
Number of pages12
Volume1448
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1448
ISSN (Print)10643745

Fingerprint

Exosomes
Cardiac Myocytes
Conditioned Culture Medium
MicroRNAs
Research
Urine
Costs and Cost Analysis
Messenger RNA
Membranes

Keywords

  • Acetyl choline esterase
  • Cardiac myocytes
  • Cell signaling
  • Exosomes
  • Heart
  • Protocol

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Malik, Z. A., Liu, T. T., & Knowlton, A. A. (2016). Cardiac myocyte exosome isolation. In Methods in Molecular Biology (Vol. 1448, pp. 237-248). (Methods in Molecular Biology; Vol. 1448). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3753-0_17

Cardiac myocyte exosome isolation. / Malik, Zulfiqar A.; Liu, Tingting T.; Knowlton, Anne A.

Methods in Molecular Biology. Vol. 1448 Humana Press Inc., 2016. p. 237-248 (Methods in Molecular Biology; Vol. 1448).

Research output: Chapter in Book/Report/Conference proceedingChapter

Malik, ZA, Liu, TT & Knowlton, AA 2016, Cardiac myocyte exosome isolation. in Methods in Molecular Biology. vol. 1448, Methods in Molecular Biology, vol. 1448, Humana Press Inc., pp. 237-248. https://doi.org/10.1007/978-1-4939-3753-0_17
Malik ZA, Liu TT, Knowlton AA. Cardiac myocyte exosome isolation. In Methods in Molecular Biology. Vol. 1448. Humana Press Inc. 2016. p. 237-248. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3753-0_17
Malik, Zulfiqar A. ; Liu, Tingting T. ; Knowlton, Anne A. / Cardiac myocyte exosome isolation. Methods in Molecular Biology. Vol. 1448 Humana Press Inc., 2016. pp. 237-248 (Methods in Molecular Biology).
@inbook{b5f6ed78a0ad4a84a825f286ce7b53ec,
title = "Cardiac myocyte exosome isolation",
abstract = "Exosomes are cell-derived small extracellular membrane vesicles (50–100 nm in diameter) actively secreted by a number of healthy and diseased cell types. Exosomes can mediate cellular, tissue, and organ level micro communication under normal and pathological conditions by shuttling proteins, mRNA, and microRNAs. Prior to vesicle molecular profiling, these exosomes can be isolated from conditioned cell media or bodily fluids such as urine and plasma in order to explore the contents and functional relevance. Exosome purification and analyses are a fast-growing research field. Regardless of several advances in exosome purification and analyses methods, research still faces several challenges. Despite tremendous interest in the role of extracellular vesicles, there is no general agreement on dependable isolation protocols. Therefore, there is an urgent need to establish reliable protocol of exosome purification and analysis. Here, we report a simple cost-effective isolation and analysis of cardiac myocyte exosomes from conditioned media.",
keywords = "Acetyl choline esterase, Cardiac myocytes, Cell signaling, Exosomes, Heart, Protocol",
author = "Malik, {Zulfiqar A.} and Liu, {Tingting T.} and Knowlton, {Anne A.}",
year = "2016",
doi = "10.1007/978-1-4939-3753-0_17",
language = "English (US)",
volume = "1448",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "237--248",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Cardiac myocyte exosome isolation

AU - Malik, Zulfiqar A.

AU - Liu, Tingting T.

AU - Knowlton, Anne A.

PY - 2016

Y1 - 2016

N2 - Exosomes are cell-derived small extracellular membrane vesicles (50–100 nm in diameter) actively secreted by a number of healthy and diseased cell types. Exosomes can mediate cellular, tissue, and organ level micro communication under normal and pathological conditions by shuttling proteins, mRNA, and microRNAs. Prior to vesicle molecular profiling, these exosomes can be isolated from conditioned cell media or bodily fluids such as urine and plasma in order to explore the contents and functional relevance. Exosome purification and analyses are a fast-growing research field. Regardless of several advances in exosome purification and analyses methods, research still faces several challenges. Despite tremendous interest in the role of extracellular vesicles, there is no general agreement on dependable isolation protocols. Therefore, there is an urgent need to establish reliable protocol of exosome purification and analysis. Here, we report a simple cost-effective isolation and analysis of cardiac myocyte exosomes from conditioned media.

AB - Exosomes are cell-derived small extracellular membrane vesicles (50–100 nm in diameter) actively secreted by a number of healthy and diseased cell types. Exosomes can mediate cellular, tissue, and organ level micro communication under normal and pathological conditions by shuttling proteins, mRNA, and microRNAs. Prior to vesicle molecular profiling, these exosomes can be isolated from conditioned cell media or bodily fluids such as urine and plasma in order to explore the contents and functional relevance. Exosome purification and analyses are a fast-growing research field. Regardless of several advances in exosome purification and analyses methods, research still faces several challenges. Despite tremendous interest in the role of extracellular vesicles, there is no general agreement on dependable isolation protocols. Therefore, there is an urgent need to establish reliable protocol of exosome purification and analysis. Here, we report a simple cost-effective isolation and analysis of cardiac myocyte exosomes from conditioned media.

KW - Acetyl choline esterase

KW - Cardiac myocytes

KW - Cell signaling

KW - Exosomes

KW - Heart

KW - Protocol

UR - http://www.scopus.com/inward/record.url?scp=84979225470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979225470&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3753-0_17

DO - 10.1007/978-1-4939-3753-0_17

M3 - Chapter

C2 - 27317185

AN - SCOPUS:84979225470

VL - 1448

T3 - Methods in Molecular Biology

SP - 237

EP - 248

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -