Abstract
Although responsible for only approximately one-third of the overall myocardial mass, the interstitial fibroblasts of the heart serve a fundamental role in establishing the functional integrity of myocardium and are the major source of myocardial extracellular matrix production. Their importance in clinical medicine is underscored by the observation that fibroblast numbers increase in response to several pathologic circumstances that are associated with an increase in extracellular matrix production, such as long standing hypertension and myocardial injury/infarction. Up to the present time, however, there has been little information available on either the kinetics of the cardiac fibroblast cell cycle, or the fundamental mechanisms that regulate its entry into and exit from the cell cycle. Previous work from our laboratory examining the effects of interleukin (IL)- 1β on myocardial growth and gene expression in culture indicated that cardiac fibroblasts have a diminished capacity to synthesize DNA in response to mitogen in the presence of this cytokine. The mechanism of IL-1β action was not clear, however, and could have resulted from action at several different points in the cell cycle. The investigations described in this report indicate that IL-1β exerts its effect on the fibroblast cell cycle at multiple levels through altering the expression of cardiac fibroblast cyclins, cyclin-dependent kinases, and their inhibitors, which ultimately affect the phosphorylation of the retinoblastoma gene product.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 25796-25803 |
| Number of pages | 8 |
| Journal | Journal of Biological Chemistry |
| Volume | 273 |
| Issue number | 40 |
| DOIs | |
| State | Published - Oct 2 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Cardiac fibroblasts arrest at the G1/S restriction point in response to interleukin (IL)-1β : Evidence for IL-1β-induced hypophosphorylation of the retinoblastoma protein. / Koudssi, Farid; Lopez, Javier E; Villegas, Sonia; Long, Carlin S.
In: Journal of Biological Chemistry, Vol. 273, No. 40, 02.10.1998, p. 25796-25803.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cardiac fibroblasts arrest at the G1/S restriction point in response to interleukin (IL)-1β
T2 - Evidence for IL-1β-induced hypophosphorylation of the retinoblastoma protein
AU - Koudssi, Farid
AU - Lopez, Javier E
AU - Villegas, Sonia
AU - Long, Carlin S.
PY - 1998/10/2
Y1 - 1998/10/2
N2 - Although responsible for only approximately one-third of the overall myocardial mass, the interstitial fibroblasts of the heart serve a fundamental role in establishing the functional integrity of myocardium and are the major source of myocardial extracellular matrix production. Their importance in clinical medicine is underscored by the observation that fibroblast numbers increase in response to several pathologic circumstances that are associated with an increase in extracellular matrix production, such as long standing hypertension and myocardial injury/infarction. Up to the present time, however, there has been little information available on either the kinetics of the cardiac fibroblast cell cycle, or the fundamental mechanisms that regulate its entry into and exit from the cell cycle. Previous work from our laboratory examining the effects of interleukin (IL)- 1β on myocardial growth and gene expression in culture indicated that cardiac fibroblasts have a diminished capacity to synthesize DNA in response to mitogen in the presence of this cytokine. The mechanism of IL-1β action was not clear, however, and could have resulted from action at several different points in the cell cycle. The investigations described in this report indicate that IL-1β exerts its effect on the fibroblast cell cycle at multiple levels through altering the expression of cardiac fibroblast cyclins, cyclin-dependent kinases, and their inhibitors, which ultimately affect the phosphorylation of the retinoblastoma gene product.
AB - Although responsible for only approximately one-third of the overall myocardial mass, the interstitial fibroblasts of the heart serve a fundamental role in establishing the functional integrity of myocardium and are the major source of myocardial extracellular matrix production. Their importance in clinical medicine is underscored by the observation that fibroblast numbers increase in response to several pathologic circumstances that are associated with an increase in extracellular matrix production, such as long standing hypertension and myocardial injury/infarction. Up to the present time, however, there has been little information available on either the kinetics of the cardiac fibroblast cell cycle, or the fundamental mechanisms that regulate its entry into and exit from the cell cycle. Previous work from our laboratory examining the effects of interleukin (IL)- 1β on myocardial growth and gene expression in culture indicated that cardiac fibroblasts have a diminished capacity to synthesize DNA in response to mitogen in the presence of this cytokine. The mechanism of IL-1β action was not clear, however, and could have resulted from action at several different points in the cell cycle. The investigations described in this report indicate that IL-1β exerts its effect on the fibroblast cell cycle at multiple levels through altering the expression of cardiac fibroblast cyclins, cyclin-dependent kinases, and their inhibitors, which ultimately affect the phosphorylation of the retinoblastoma gene product.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032476045&partnerID=8YFLogxK
U2 - 10.1074/jbc.273.40.25796
DO - 10.1074/jbc.273.40.25796
M3 - Article
C2 - 9748252
AN - SCOPUS:0032476045
VL - 273
SP - 25796
EP - 25803
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 40
ER -