Cardiac applications of second harmonic generation (SHG) microscopy

Che Wei Chang, Hannah A. Ledford, Hillary K.J. Kao, Xiao Dong Zhang, Nipavan Chiamvimonvat, Deborah Lieu, James W Chan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) are an unlimited ex vivo supply of heart cells for cardiac applications. The establishment of pure iPSC-CMs populations is crucial for downstream medical applications such as human disease modeling, patient-specific stem cell therapy, human transplantation, and drug development. However, a significant challenge is the lack of an established purification method to isolate populations of iPSC-CMs by their phenotype, maturity, and subtype due to the lack of specific iPSC-CM markers. The ability to remove potentially teratoma forming pluripotent stem cells, arrhythmia inducing immature and pacemaking cells, and other non-CMs is extremely important for engineering tissues with desired cell compositions that are both safe for human transplantation and that can accurately replicate cardiac functions. Contemporary purification techniques have either low specificity or require genetic modification. We have proposed that second harmonic generation (SHG) signals, which are known to originate from the sarcomeric myosin filaments in cardiomyocytes, can be a highly specific, labelfree marker for identifying iPSC-CMs. Here, we demonstrate the use of SHG microscopy for characterizing iPSC-CMs and their subtypes.

Original languageEnglish (US)
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIX
EditorsPeter T. C. So, Karsten Konig, Ammasi Periasamy
PublisherSPIE
ISBN (Electronic)9781510624061
DOIs
StatePublished - Jan 1 2019
EventMultiphoton Microscopy in the Biomedical Sciences XIX 2019 - San Francisco, United States
Duration: Feb 3 2019Feb 6 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10882
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIX 2019
CountryUnited States
CitySan Francisco
Period2/3/192/6/19

Fingerprint

stem cells
Induced Pluripotent Stem Cells
Harmonic generation
Stem cells
Cardiac Myocytes
Microscopy
harmonic generations
Microscopic examination
microscopy
transplantation
purification
markers
Purification
Transplantation
cells
Pluripotent Stem Cells
arrhythmia
myosins
Teratoma
Tissue Engineering

Keywords

  • Action potential
  • Cardiomyocytes
  • Heart muscle
  • Induced pluripotent stem cells
  • Myosin
  • Second harmonic generation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Chang, C. W., Ledford, H. A., Kao, H. K. J., Zhang, X. D., Chiamvimonvat, N., Lieu, D., & Chan, J. W. (2019). Cardiac applications of second harmonic generation (SHG) microscopy. In P. T. C. So, K. Konig, & A. Periasamy (Eds.), Multiphoton Microscopy in the Biomedical Sciences XIX [1088213] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10882). SPIE. https://doi.org/10.1117/12.2510387

Cardiac applications of second harmonic generation (SHG) microscopy. / Chang, Che Wei; Ledford, Hannah A.; Kao, Hillary K.J.; Zhang, Xiao Dong; Chiamvimonvat, Nipavan; Lieu, Deborah; Chan, James W.

Multiphoton Microscopy in the Biomedical Sciences XIX. ed. / Peter T. C. So; Karsten Konig; Ammasi Periasamy. SPIE, 2019. 1088213 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10882).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chang, CW, Ledford, HA, Kao, HKJ, Zhang, XD, Chiamvimonvat, N, Lieu, D & Chan, JW 2019, Cardiac applications of second harmonic generation (SHG) microscopy. in PTC So, K Konig & A Periasamy (eds), Multiphoton Microscopy in the Biomedical Sciences XIX., 1088213, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10882, SPIE, Multiphoton Microscopy in the Biomedical Sciences XIX 2019, San Francisco, United States, 2/3/19. https://doi.org/10.1117/12.2510387
Chang CW, Ledford HA, Kao HKJ, Zhang XD, Chiamvimonvat N, Lieu D et al. Cardiac applications of second harmonic generation (SHG) microscopy. In So PTC, Konig K, Periasamy A, editors, Multiphoton Microscopy in the Biomedical Sciences XIX. SPIE. 2019. 1088213. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2510387
Chang, Che Wei ; Ledford, Hannah A. ; Kao, Hillary K.J. ; Zhang, Xiao Dong ; Chiamvimonvat, Nipavan ; Lieu, Deborah ; Chan, James W. / Cardiac applications of second harmonic generation (SHG) microscopy. Multiphoton Microscopy in the Biomedical Sciences XIX. editor / Peter T. C. So ; Karsten Konig ; Ammasi Periasamy. SPIE, 2019. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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