Cardiac adaptations in SCNT newborn cloned calves during the first month of life assessed by echocardiography

C. A. Batchelder, Mary B Whitcomb, T. R. Famula, P. Rodriguez-Villamil, M. Bertolini, K. A. Hoffert-Goeres, G. B. Anderson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aim of this study was to compare cardiac morphology in newborn and month-old control and cloned calves. A total of 10 in vivo-derived (IVD) control (five Holstein, five Hereford) and seven cloned (five Holstein, two Hereford) calves were subjected to echocardiographic examination, including 2D, M-mode, spectral and color flow Doppler evaluation at Day 1 (mean 26.3 h) and Day 30 (mean 29.2 days) after birth. Echocardiographic measurements were compared between control and cloned calves, and between Hereford and Holstein control calves of the same age. At Day 1 and at Day 30 after birth, left ventricular free wall (LVFW) and interventricular septal (IVS) thicknesses were greater in Holstein calves than Hereford calves. Several indices of myocardial wall thickness were increased in cloned versus control calves at Day 1 after birth, and included systolic LVFW thickness, systolic right ventricular free wall (RVFW) thickness, diastolic LVFW thickness, diastolic RVFW thickness and diastolic IVS thickness (p < 0.05). Differences between cloned and non-cloned calves were no longer evident at Day 30 after birth. The apparent disappearance of the cloning effect on cardiac structures may reflect the influence of placenta on fetal cardiac morphology, suggestive of a placental hemodynamic role in fetal cardiac muscle development. Differences seen in clones at birth spontaneously resolved by Day 30 of age, after organ development recovery from cardiovascular abnormalities of presumed placental origin. Echocardiographic measurements should provide useful data for research and clinical evaluation of high-risk neonatal calves of both breeds and from clones of the same breed.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalTheriogenology
Volume103
DOIs
StatePublished - Nov 1 2017

Fingerprint

echocardiography
Echocardiography
neonates
Parturition
calves
Hereford
Holstein
Clone Cells
Cardiovascular Abnormalities
Muscle Development
Placenta
Organism Cloning
Myocardium
Color
clones
Hemodynamics
breeds
muscle development
hemodynamics
myocardium

Keywords

  • Bovine
  • Cloning
  • Echocardiographic measurements
  • Neonatal
  • Placenta
  • Ultrasound

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Batchelder, C. A., Whitcomb, M. B., Famula, T. R., Rodriguez-Villamil, P., Bertolini, M., Hoffert-Goeres, K. A., & Anderson, G. B. (2017). Cardiac adaptations in SCNT newborn cloned calves during the first month of life assessed by echocardiography. Theriogenology, 103, 153-161. https://doi.org/10.1016/j.theriogenology.2017.07.023

Cardiac adaptations in SCNT newborn cloned calves during the first month of life assessed by echocardiography. / Batchelder, C. A.; Whitcomb, Mary B; Famula, T. R.; Rodriguez-Villamil, P.; Bertolini, M.; Hoffert-Goeres, K. A.; Anderson, G. B.

In: Theriogenology, Vol. 103, 01.11.2017, p. 153-161.

Research output: Contribution to journalArticle

Batchelder, CA, Whitcomb, MB, Famula, TR, Rodriguez-Villamil, P, Bertolini, M, Hoffert-Goeres, KA & Anderson, GB 2017, 'Cardiac adaptations in SCNT newborn cloned calves during the first month of life assessed by echocardiography', Theriogenology, vol. 103, pp. 153-161. https://doi.org/10.1016/j.theriogenology.2017.07.023
Batchelder, C. A. ; Whitcomb, Mary B ; Famula, T. R. ; Rodriguez-Villamil, P. ; Bertolini, M. ; Hoffert-Goeres, K. A. ; Anderson, G. B. / Cardiac adaptations in SCNT newborn cloned calves during the first month of life assessed by echocardiography. In: Theriogenology. 2017 ; Vol. 103. pp. 153-161.
@article{53c2dfe5a3bc474481c978ef889b2be8,
title = "Cardiac adaptations in SCNT newborn cloned calves during the first month of life assessed by echocardiography",
abstract = "The aim of this study was to compare cardiac morphology in newborn and month-old control and cloned calves. A total of 10 in vivo-derived (IVD) control (five Holstein, five Hereford) and seven cloned (five Holstein, two Hereford) calves were subjected to echocardiographic examination, including 2D, M-mode, spectral and color flow Doppler evaluation at Day 1 (mean 26.3 h) and Day 30 (mean 29.2 days) after birth. Echocardiographic measurements were compared between control and cloned calves, and between Hereford and Holstein control calves of the same age. At Day 1 and at Day 30 after birth, left ventricular free wall (LVFW) and interventricular septal (IVS) thicknesses were greater in Holstein calves than Hereford calves. Several indices of myocardial wall thickness were increased in cloned versus control calves at Day 1 after birth, and included systolic LVFW thickness, systolic right ventricular free wall (RVFW) thickness, diastolic LVFW thickness, diastolic RVFW thickness and diastolic IVS thickness (p < 0.05). Differences between cloned and non-cloned calves were no longer evident at Day 30 after birth. The apparent disappearance of the cloning effect on cardiac structures may reflect the influence of placenta on fetal cardiac morphology, suggestive of a placental hemodynamic role in fetal cardiac muscle development. Differences seen in clones at birth spontaneously resolved by Day 30 of age, after organ development recovery from cardiovascular abnormalities of presumed placental origin. Echocardiographic measurements should provide useful data for research and clinical evaluation of high-risk neonatal calves of both breeds and from clones of the same breed.",
keywords = "Bovine, Cloning, Echocardiographic measurements, Neonatal, Placenta, Ultrasound",
author = "Batchelder, {C. A.} and Whitcomb, {Mary B} and Famula, {T. R.} and P. Rodriguez-Villamil and M. Bertolini and Hoffert-Goeres, {K. A.} and Anderson, {G. B.}",
year = "2017",
month = "11",
day = "1",
doi = "10.1016/j.theriogenology.2017.07.023",
language = "English (US)",
volume = "103",
pages = "153--161",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Cardiac adaptations in SCNT newborn cloned calves during the first month of life assessed by echocardiography

AU - Batchelder, C. A.

AU - Whitcomb, Mary B

AU - Famula, T. R.

AU - Rodriguez-Villamil, P.

AU - Bertolini, M.

AU - Hoffert-Goeres, K. A.

AU - Anderson, G. B.

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The aim of this study was to compare cardiac morphology in newborn and month-old control and cloned calves. A total of 10 in vivo-derived (IVD) control (five Holstein, five Hereford) and seven cloned (five Holstein, two Hereford) calves were subjected to echocardiographic examination, including 2D, M-mode, spectral and color flow Doppler evaluation at Day 1 (mean 26.3 h) and Day 30 (mean 29.2 days) after birth. Echocardiographic measurements were compared between control and cloned calves, and between Hereford and Holstein control calves of the same age. At Day 1 and at Day 30 after birth, left ventricular free wall (LVFW) and interventricular septal (IVS) thicknesses were greater in Holstein calves than Hereford calves. Several indices of myocardial wall thickness were increased in cloned versus control calves at Day 1 after birth, and included systolic LVFW thickness, systolic right ventricular free wall (RVFW) thickness, diastolic LVFW thickness, diastolic RVFW thickness and diastolic IVS thickness (p < 0.05). Differences between cloned and non-cloned calves were no longer evident at Day 30 after birth. The apparent disappearance of the cloning effect on cardiac structures may reflect the influence of placenta on fetal cardiac morphology, suggestive of a placental hemodynamic role in fetal cardiac muscle development. Differences seen in clones at birth spontaneously resolved by Day 30 of age, after organ development recovery from cardiovascular abnormalities of presumed placental origin. Echocardiographic measurements should provide useful data for research and clinical evaluation of high-risk neonatal calves of both breeds and from clones of the same breed.

AB - The aim of this study was to compare cardiac morphology in newborn and month-old control and cloned calves. A total of 10 in vivo-derived (IVD) control (five Holstein, five Hereford) and seven cloned (five Holstein, two Hereford) calves were subjected to echocardiographic examination, including 2D, M-mode, spectral and color flow Doppler evaluation at Day 1 (mean 26.3 h) and Day 30 (mean 29.2 days) after birth. Echocardiographic measurements were compared between control and cloned calves, and between Hereford and Holstein control calves of the same age. At Day 1 and at Day 30 after birth, left ventricular free wall (LVFW) and interventricular septal (IVS) thicknesses were greater in Holstein calves than Hereford calves. Several indices of myocardial wall thickness were increased in cloned versus control calves at Day 1 after birth, and included systolic LVFW thickness, systolic right ventricular free wall (RVFW) thickness, diastolic LVFW thickness, diastolic RVFW thickness and diastolic IVS thickness (p < 0.05). Differences between cloned and non-cloned calves were no longer evident at Day 30 after birth. The apparent disappearance of the cloning effect on cardiac structures may reflect the influence of placenta on fetal cardiac morphology, suggestive of a placental hemodynamic role in fetal cardiac muscle development. Differences seen in clones at birth spontaneously resolved by Day 30 of age, after organ development recovery from cardiovascular abnormalities of presumed placental origin. Echocardiographic measurements should provide useful data for research and clinical evaluation of high-risk neonatal calves of both breeds and from clones of the same breed.

KW - Bovine

KW - Cloning

KW - Echocardiographic measurements

KW - Neonatal

KW - Placenta

KW - Ultrasound

UR - http://www.scopus.com/inward/record.url?scp=85026745886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026745886&partnerID=8YFLogxK

U2 - 10.1016/j.theriogenology.2017.07.023

DO - 10.1016/j.theriogenology.2017.07.023

M3 - Article

C2 - 28787665

AN - SCOPUS:85026745886

VL - 103

SP - 153

EP - 161

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

ER -