Carboxyfluorescein as a probe for liposome-cell interactions effect of impurities, and purification of the dye

Evelyn Ralston, Leonard M Hjelmeland, Richard D. Klausner, John N. Weinstein, Robert Blumenthal

Research output: Contribution to journalArticle

254 Scopus citations

Abstract

Impurities in 5(6)-carboxyfluorescein can affect phospholipid vesicle stability and apparent rates of carboxyfluorescein transfer into cells. Thorough purification and characterization of the dye are thus important to many applications with vesicles and/or cells. The dye can be purified by adsorption chromatography on a hydrophobic gel, following treatment with activated charcoal and precipitation from ethanol-water. The 5- and 6-carboxy-isomers can be separated from each other (though for most purposes it is not necessary to do so) by synthesis, crystallization, and hydrolysis of the diacetate derivatives. Purification is monitored by thin-layer and high pressure chromatography.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalBBA - Biomembranes
Volume649
Issue number1
DOIs
StatePublished - Nov 20 1981
Externally publishedYes

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Keywords

  • Carboxyfluorescein impurity
  • Liposome-cell interaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

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