Carboxyfluorescein as a probe for liposome-cell interactions effect of impurities, and purification of the dye

Evelyn Ralston; Leonard M Hjelmeland; Richard D. Klausner; John N. Weinstein; Robert Blumenthal

doi:10.1016/0005-2736(81)90019-5

Carboxyfluorescein as a probe for liposome-cell interactions effect of impurities, and purification of the dye

Evelyn Ralston, Leonard M Hjelmeland, Richard D. Klausner, John N. Weinstein, Robert Blumenthal

Research output: Contribution to journal › Article › peer-review

254 Scopus citations

Abstract

Impurities in 5(6)-carboxyfluorescein can affect phospholipid vesicle stability and apparent rates of carboxyfluorescein transfer into cells. Thorough purification and characterization of the dye are thus important to many applications with vesicles and/or cells. The dye can be purified by adsorption chromatography on a hydrophobic gel, following treatment with activated charcoal and precipitation from ethanol-water. The 5- and 6-carboxy-isomers can be separated from each other (though for most purposes it is not necessary to do so) by synthesis, crystallization, and hydrolysis of the diacetate derivatives. Purification is monitored by thin-layer and high pressure chromatography.

Original language	English (US)
Pages (from-to)	133-137
Number of pages	5
Journal	BBA - Biomembranes
Volume	649
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(81)90019-5
State	Published - Nov 20 1981
Externally published	Yes

Keywords

Carboxyfluorescein impurity
Liposome-cell interaction

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology

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10.1016/0005-2736(81)90019-5

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title = "Carboxyfluorescein as a probe for liposome-cell interactions effect of impurities, and purification of the dye",

abstract = "Impurities in 5(6)-carboxyfluorescein can affect phospholipid vesicle stability and apparent rates of carboxyfluorescein transfer into cells. Thorough purification and characterization of the dye are thus important to many applications with vesicles and/or cells. The dye can be purified by adsorption chromatography on a hydrophobic gel, following treatment with activated charcoal and precipitation from ethanol-water. The 5- and 6-carboxy-isomers can be separated from each other (though for most purposes it is not necessary to do so) by synthesis, crystallization, and hydrolysis of the diacetate derivatives. Purification is monitored by thin-layer and high pressure chromatography.",

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T1 - Carboxyfluorescein as a probe for liposome-cell interactions effect of impurities, and purification of the dye

AU - Ralston, Evelyn

AU - Hjelmeland, Leonard M

AU - Klausner, Richard D.

AU - Weinstein, John N.

AU - Blumenthal, Robert

PY - 1981/11/20

Y1 - 1981/11/20

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AB - Impurities in 5(6)-carboxyfluorescein can affect phospholipid vesicle stability and apparent rates of carboxyfluorescein transfer into cells. Thorough purification and characterization of the dye are thus important to many applications with vesicles and/or cells. The dye can be purified by adsorption chromatography on a hydrophobic gel, following treatment with activated charcoal and precipitation from ethanol-water. The 5- and 6-carboxy-isomers can be separated from each other (though for most purposes it is not necessary to do so) by synthesis, crystallization, and hydrolysis of the diacetate derivatives. Purification is monitored by thin-layer and high pressure chromatography.

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