Abstract
Impurities in 5(6)-carboxyfluorescein can affect phospholipid vesicle stability and apparent rates of carboxyfluorescein transfer into cells. Thorough purification and characterization of the dye are thus important to many applications with vesicles and/or cells. The dye can be purified by adsorption chromatography on a hydrophobic gel, following treatment with activated charcoal and precipitation from ethanol-water. The 5- and 6-carboxy-isomers can be separated from each other (though for most purposes it is not necessary to do so) by synthesis, crystallization, and hydrolysis of the diacetate derivatives. Purification is monitored by thin-layer and high pressure chromatography.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 133-137 |
| Number of pages | 5 |
| Journal | BBA - Biomembranes |
| Volume | 649 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 20 1981 |
| Externally published | Yes |
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Keywords
- Carboxyfluorescein impurity
- Liposome-cell interaction
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
Cite this
Carboxyfluorescein as a probe for liposome-cell interactions effect of impurities, and purification of the dye. / Ralston, Evelyn; Hjelmeland, Leonard M; Klausner, Richard D.; Weinstein, John N.; Blumenthal, Robert.
In: BBA - Biomembranes, Vol. 649, No. 1, 20.11.1981, p. 133-137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Carboxyfluorescein as a probe for liposome-cell interactions effect of impurities, and purification of the dye
AU - Ralston, Evelyn
AU - Hjelmeland, Leonard M
AU - Klausner, Richard D.
AU - Weinstein, John N.
AU - Blumenthal, Robert
PY - 1981/11/20
Y1 - 1981/11/20
N2 - Impurities in 5(6)-carboxyfluorescein can affect phospholipid vesicle stability and apparent rates of carboxyfluorescein transfer into cells. Thorough purification and characterization of the dye are thus important to many applications with vesicles and/or cells. The dye can be purified by adsorption chromatography on a hydrophobic gel, following treatment with activated charcoal and precipitation from ethanol-water. The 5- and 6-carboxy-isomers can be separated from each other (though for most purposes it is not necessary to do so) by synthesis, crystallization, and hydrolysis of the diacetate derivatives. Purification is monitored by thin-layer and high pressure chromatography.
AB - Impurities in 5(6)-carboxyfluorescein can affect phospholipid vesicle stability and apparent rates of carboxyfluorescein transfer into cells. Thorough purification and characterization of the dye are thus important to many applications with vesicles and/or cells. The dye can be purified by adsorption chromatography on a hydrophobic gel, following treatment with activated charcoal and precipitation from ethanol-water. The 5- and 6-carboxy-isomers can be separated from each other (though for most purposes it is not necessary to do so) by synthesis, crystallization, and hydrolysis of the diacetate derivatives. Purification is monitored by thin-layer and high pressure chromatography.
KW - Carboxyfluorescein impurity
KW - Liposome-cell interaction
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UR - http://www.scopus.com/inward/citedby.url?scp=0011042466&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(81)90019-5
DO - 10.1016/0005-2736(81)90019-5
M3 - Article
VL - 649
SP - 133
EP - 137
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 1
ER -