Carbon turnover in the water-soluble protein of the adult human lens

Daniel N. Stewart, Jozsef Lango, Krishnan P. Nambiar, Miranda J S Falso, Paul G FitzGerald, David M Rocke, Bruce D. Hammock, Bruce A. Buchholz

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Purpose: Human eye lenses contain cells that persist from embryonic development. These unique, highly specialized fiber cells located at the core (nucleus) of the lens undergo pseudo-apoptosis to become devoid of cell nuclei and most organelles. Ostensibly lacking in protein transcriptional capabilities, it is currently believed that these nuclear fiber cells owe their extreme longevity to the perseverance of highly stable and densely packed crystallin proteins. Maintaining the structural and functional integrity of lenticular proteins is necessary to sustain cellular transparency and proper vision, yet the means by which the lens actually copes with a lifetime of oxidative stress, seemingly without any capacity for protein turnover and repair, is not completely understood. Although many years of research have been predicated upon the assumption that there is no protein turnover or renewal in nuclear fiber cells, we investigated whether or not different protein fractions possess protein of different ages by using the 14C bomb pulse. Methods: Adult human lenses were concentrically dissected by gently removing the cell layers in water or shaving to the nucleus with a curved micrometer-controlled blade. The cells were lysed, and the proteins were separated into water-soluble and water-insoluble fractions. The small molecules were removed using 3kDa spin filters. The 14C/C was measured in paired protein fractions by accelerator mass spectrometry, and an average age for the material within the sample was assigned using the 14C bomb pulse. Results: The water-insoluble fractions possessed 14C/C ratios consistent with the age of the cells. In all cases, the water-soluble fractions contained carbon that was younger than the paired water-insoluble fraction. Conclusions: As the first direct evidence of carbon turnover in protein from adult human nuclear fiber cells, this discoery supports the emerging view of the lens nucleus as a dynamic system capable of maintaining homeostasis in part due to intricate protein transport mechanisms and possibly protein repair. This finding implies that the lens plays an active role in the aversion of age-related nuclear (ARN) cataract.

Original languageEnglish (US)
Pages (from-to)463-475
Number of pages13
JournalMolecular Vision
Volume19
StatePublished - Feb 25 2013

ASJC Scopus subject areas

  • Ophthalmology

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    Stewart, D. N., Lango, J., Nambiar, K. P., Falso, M. J. S., FitzGerald, P. G., Rocke, D. M., Hammock, B. D., & Buchholz, B. A. (2013). Carbon turnover in the water-soluble protein of the adult human lens. Molecular Vision, 19, 463-475.