Carbohydrate metabolism of the rat C6 glioma. An in vivo 13C and in vitro 1H magnetic resonance spectroscopy study.

B. D. Ross, Robert Higgins, James E Boggan, J. A. Willis, B. Knittel, S. W. Unger

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Abstract

Surface coil 13C nuclear magnetic resonance (NMR) spectroscopy was used to investigate the in vivo carbohydrate metabolism of rat C6 gliomas during and after infusion with [1-13C] glucose. In vivo 1H-decoupled 13C NMR spectra of the glioma following infusion with [1-13C]glucose revealed the direct production of [3-13C]lactic acid, [1-13C]glycogen, and [4-13C], [3-13C], and [2-13C]glutamate/glutamine. Lactate levels of in vivo gliomas increased and reached steady state levels during [1-13C]glucose infusion, and decreased following termination of infusion. Complementary in vitro studies using supernatant media collected from C6 glioma cells incubated with media containing [1-13C] or [6-13C]glucose and glutamine were examined by 1H NMR spectroscopy. The [3-(13C/12C)]lactate ratios obtained from 1H spectra of supernatant media containing [1-13C]glucose revealed the percentage of glucose metabolized through the hexose monophosphate shunt to be 10.01 +/- 0.85% (n = 3), while similar measurements of media containing [6-13C]glucose and glutamine showed that glutaminolysis contributed 9.0 +/- 1.0% of total lactate production under these conditions. Enzymatic analysis of media determined lactate production to be 139 +/- 9 nmol per 10(6) cells per h (n = 4). These measurements demonstrate the ability of NMR to monitor brain tumor carbohydrate metabolism both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)20-26
Number of pages7
JournalNMR in Biomedicine
Volume1
Issue number1
StatePublished - Feb 1988

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Magnetic resonance spectroscopy
Carbohydrate Metabolism
Glioma
Rats
Magnetic Resonance Spectroscopy
Glucose
Lactic Acid
Glutamine
Nuclear magnetic resonance spectroscopy
Nuclear magnetic resonance
Pentose Phosphate Pathway
Hexoses
In Vitro Techniques
Glycogen
Brain Neoplasms
Tumors
Glutamic Acid
Brain

ASJC Scopus subject areas

  • Biophysics

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Carbohydrate metabolism of the rat C6 glioma. An in vivo 13C and in vitro 1H magnetic resonance spectroscopy study. / Ross, B. D.; Higgins, Robert; Boggan, James E; Willis, J. A.; Knittel, B.; Unger, S. W.

In: NMR in Biomedicine, Vol. 1, No. 1, 02.1988, p. 20-26.

Research output: Contribution to journalArticle

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