Capture Hi-C: Characterization of chromatin contacts

Ryan R. Davis, Mel Campbell, Yoshihiro Izumiya, Clifford G Tepper

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Hi-C (high throughput chromosome conformation capture) is a powerful tool designed to explore the genome-wide collection of chromosomal contacts within a cell population. However, the highly complex nature of Hi-C libraries often precludes the robust identification of interactions between individual restriction fragments with high resolution and statistical significance. This challenge led to the incorporation of sequence capture technology into Hi-C protocols, giving rise to a Capture Hi-C methodology. In this chapter, we review the general principles of the technique and provide a view emanating from our experience employing this approach to study the dynamics of episomal viral DNA contacts during specific stages of the viral life cycle.

Original languageEnglish (US)
Title of host publicationEpigenetics Methods
PublisherElsevier
Pages419-444
Number of pages26
ISBN (Electronic)9780128194140
DOIs
StatePublished - Jan 1 2020

Keywords

  • Chromatin
  • Computational analysis
  • Episome
  • Herpesvirus
  • Kaposi’s sarcoma-associated herpesvirus
  • Nucleome
  • Reactivation
  • Sequence capture
  • Target enrichment

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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