Capture and detection of T7 bacteriophages on a nanostructured interface

Jin Hee Han, Min S. Wang, Jayanti Das, L. Sudheendra, Erica Vonasek, Nitin Nitin, Ian M. Kennedy

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A highly ordered array of T7 bacteriophages was created by the electrophoretic capture of phages onto a nanostructured array with wells that accommodated the phages. Electrophoresis of bacteriophages was achieved by applying a positive potential on an indium tin oxide electrode at the bottom of the nanowells. Nanoscale arrays of phages with different surface densities were obtained by changing the electric field applied to the bottom of the nanowells. The applied voltage was shown to be the critical factor in generating a well-ordered phage array. The number of wells occupied by a phage, and hence the concentration of phages in a sample solution, could be quantified by using a DNA intercalating dye that rapidly stains the T7 phage. The fluorescence signal was enhanced by the intrinsic photonic effect made available by the geometry of the platform. It was shown that the quantification of phages on the array was 6 orders of magnitude better than could be obtained with a fluorescent plate reader. The device opens up the possibility that phages can be detected directly without enrichment or culturing, and by detecting phages that specifically infect bacteria of interest, rapid pathogen detection becomes possible.

Original languageEnglish (US)
Pages (from-to)4758-4765
Number of pages8
JournalACS Applied Materials and Interfaces
Volume6
Issue number7
DOIs
StatePublished - Apr 9 2014

Keywords

  • array
  • capture
  • electrophoresis
  • pathogen detection
  • photonic crystal
  • T7 bacteriophages

ASJC Scopus subject areas

  • Materials Science(all)

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  • Cite this

    Han, J. H., Wang, M. S., Das, J., Sudheendra, L., Vonasek, E., Nitin, N., & Kennedy, I. M. (2014). Capture and detection of T7 bacteriophages on a nanostructured interface. ACS Applied Materials and Interfaces, 6(7), 4758-4765. https://doi.org/10.1021/am500655r