Capture and characterization of influenza A virus from primary samples using glycan bead arrays

Miriam Cohen, Christopher J. Fisher, Mia L. Huang, Le Ann L Lindsay, Magdalena Plancarte, Walter M Boyce, Kamil Godula, Pascal Gagneux

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Influenza A viruses (IAVs) utilize sialylated host glycans as ligands for binding and infection. The glycan-binding preference of IAV hemagglutinin (HA) is an important determinant of host specificity. Propagation of IAV in embryonated chicken eggs and cultured mammalian cells yields viruses with amino acid substitutions in the HA that can alter the binding specificity. Therefore, it is important to determine the binding specificity of IAV directly in primary samples since it reflects the actual tropism of virus in nature. We developed a novel platform for analysis of IAV binding specificity in samples that contain very low virus titers. This platform consists of a high-density flexible glycan display on magnetic beads, which promotes multivalent interactions with the viral HA. Glycan-bound virus is detected by quantifying the viral neuraminidase activity via a fluorogenic reporter, 2'-(4-methylumbelliferyl)-α-. d-N-acetylneuraminic acid. This method eliminates the need for labeling the virus and significantly enhances the sensitivity of detection.

Original languageEnglish (US)
Pages (from-to)128-135
Number of pages8
JournalVirology
Volume493
DOIs
StatePublished - Jun 1 2016

Keywords

  • Glycan array
  • Glycans
  • Influenza A
  • Magnetic beads
  • Neuraminidase
  • Sialic acids

ASJC Scopus subject areas

  • Virology

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    Cohen, M., Fisher, C. J., Huang, M. L., Lindsay, L. A. L., Plancarte, M., Boyce, W. M., Godula, K., & Gagneux, P. (2016). Capture and characterization of influenza A virus from primary samples using glycan bead arrays. Virology, 493, 128-135. https://doi.org/10.1016/j.virol.2016.03.011