Capillary morphogenesis during healing of full‐thickness skin grafts: An ultrastructural study

Michael J. Goretsky, Matthew Breeden, Gregory Pisarski, M. Dana Harriger, Steven T. Boyce, David G Greenhalgh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Biologic mechanisms by which skin grafts become revascularized after transplantation are poorly understood. To investigate graft revascularization, we examined the pattern of capillary growth in full‐thickness skin grafts at serial time points. Full‐thickness skin (2 × 2 cm) was excised to muscle fascia from the bilateral hind limbs of adult male Lewis rats. The graft/wound base boundary was identified by placement of a polypropylene mesh on the wound beneath the graft. Excised skin was replaced in its original orientation and secured with silk sutures tied over a gauze bolster dressing. After 3, 5, 7, and 10 days, animals were killed, and their aortas were cannulated and infused with an acrylic polymer to generate vascular casts. Grafts were excised, tissues were digested, and casts were examined with the use of scanning electron microscopy. Transmission electron microscopy was performed on tissues infused with the acrylic polymer that were not digested. At day 3, an immature lobular pattern was observed extending from the neovascular plexi on the graft side of the polypropylene mesh. At day 5, defined vessels with lobular ends occurred with high frequency. At day 7, the number of observed lobular structures was greatly reduced, and high frequencies of depressions in acrylic casts suggested protrusion of endothelial cell nuclei. By day 10, lobular structures were rare, well‐defined microvascular plexi were contiguous with larger vessels, and depressions from endothelial cell nuclei appeared more shallow and less frequent. These findings suggest that (1) an immature lobular pattern representing either capillary outgrowth or extracapillary leakage occurs at day 3; (2) these immature lobules decrease, and more discrete capillaries increase by day 5; (3) vascular integrity is reestablished by day 7; (4) vascular plexi has regained full continuity, and there are suggestions that endothelial cell proliferation has subsided by day 10.

Original languageEnglish (US)
Pages (from-to)213-220
Number of pages8
JournalWound Repair and Regeneration
Volume3
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Morphogenesis
Transplants
Skin
Blood Vessels
Endothelial Cells
Polypropylenes
Cell Nucleus
Polymers
Silk
Fascia
Wounds and Injuries
Bandages
Transmission Electron Microscopy
Electron Scanning Microscopy
Sutures
Aorta
Extremities
Transplantation
Cell Proliferation
Muscles

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Capillary morphogenesis during healing of full‐thickness skin grafts : An ultrastructural study. / Goretsky, Michael J.; Breeden, Matthew; Pisarski, Gregory; Harriger, M. Dana; Boyce, Steven T.; Greenhalgh, David G.

In: Wound Repair and Regeneration, Vol. 3, No. 2, 1995, p. 213-220.

Research output: Contribution to journalArticle

Goretsky, Michael J. ; Breeden, Matthew ; Pisarski, Gregory ; Harriger, M. Dana ; Boyce, Steven T. ; Greenhalgh, David G. / Capillary morphogenesis during healing of full‐thickness skin grafts : An ultrastructural study. In: Wound Repair and Regeneration. 1995 ; Vol. 3, No. 2. pp. 213-220.
@article{56bc505a99cb45cb9066826c89100a7a,
title = "Capillary morphogenesis during healing of full‐thickness skin grafts: An ultrastructural study",
abstract = "Biologic mechanisms by which skin grafts become revascularized after transplantation are poorly understood. To investigate graft revascularization, we examined the pattern of capillary growth in full‐thickness skin grafts at serial time points. Full‐thickness skin (2 × 2 cm) was excised to muscle fascia from the bilateral hind limbs of adult male Lewis rats. The graft/wound base boundary was identified by placement of a polypropylene mesh on the wound beneath the graft. Excised skin was replaced in its original orientation and secured with silk sutures tied over a gauze bolster dressing. After 3, 5, 7, and 10 days, animals were killed, and their aortas were cannulated and infused with an acrylic polymer to generate vascular casts. Grafts were excised, tissues were digested, and casts were examined with the use of scanning electron microscopy. Transmission electron microscopy was performed on tissues infused with the acrylic polymer that were not digested. At day 3, an immature lobular pattern was observed extending from the neovascular plexi on the graft side of the polypropylene mesh. At day 5, defined vessels with lobular ends occurred with high frequency. At day 7, the number of observed lobular structures was greatly reduced, and high frequencies of depressions in acrylic casts suggested protrusion of endothelial cell nuclei. By day 10, lobular structures were rare, well‐defined microvascular plexi were contiguous with larger vessels, and depressions from endothelial cell nuclei appeared more shallow and less frequent. These findings suggest that (1) an immature lobular pattern representing either capillary outgrowth or extracapillary leakage occurs at day 3; (2) these immature lobules decrease, and more discrete capillaries increase by day 5; (3) vascular integrity is reestablished by day 7; (4) vascular plexi has regained full continuity, and there are suggestions that endothelial cell proliferation has subsided by day 10.",
author = "Goretsky, {Michael J.} and Matthew Breeden and Gregory Pisarski and Harriger, {M. Dana} and Boyce, {Steven T.} and Greenhalgh, {David G}",
year = "1995",
doi = "10.1046/j.1524-475X.1995.30213.x",
language = "English (US)",
volume = "3",
pages = "213--220",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Capillary morphogenesis during healing of full‐thickness skin grafts

T2 - An ultrastructural study

AU - Goretsky, Michael J.

AU - Breeden, Matthew

AU - Pisarski, Gregory

AU - Harriger, M. Dana

AU - Boyce, Steven T.

AU - Greenhalgh, David G

PY - 1995

Y1 - 1995

N2 - Biologic mechanisms by which skin grafts become revascularized after transplantation are poorly understood. To investigate graft revascularization, we examined the pattern of capillary growth in full‐thickness skin grafts at serial time points. Full‐thickness skin (2 × 2 cm) was excised to muscle fascia from the bilateral hind limbs of adult male Lewis rats. The graft/wound base boundary was identified by placement of a polypropylene mesh on the wound beneath the graft. Excised skin was replaced in its original orientation and secured with silk sutures tied over a gauze bolster dressing. After 3, 5, 7, and 10 days, animals were killed, and their aortas were cannulated and infused with an acrylic polymer to generate vascular casts. Grafts were excised, tissues were digested, and casts were examined with the use of scanning electron microscopy. Transmission electron microscopy was performed on tissues infused with the acrylic polymer that were not digested. At day 3, an immature lobular pattern was observed extending from the neovascular plexi on the graft side of the polypropylene mesh. At day 5, defined vessels with lobular ends occurred with high frequency. At day 7, the number of observed lobular structures was greatly reduced, and high frequencies of depressions in acrylic casts suggested protrusion of endothelial cell nuclei. By day 10, lobular structures were rare, well‐defined microvascular plexi were contiguous with larger vessels, and depressions from endothelial cell nuclei appeared more shallow and less frequent. These findings suggest that (1) an immature lobular pattern representing either capillary outgrowth or extracapillary leakage occurs at day 3; (2) these immature lobules decrease, and more discrete capillaries increase by day 5; (3) vascular integrity is reestablished by day 7; (4) vascular plexi has regained full continuity, and there are suggestions that endothelial cell proliferation has subsided by day 10.

AB - Biologic mechanisms by which skin grafts become revascularized after transplantation are poorly understood. To investigate graft revascularization, we examined the pattern of capillary growth in full‐thickness skin grafts at serial time points. Full‐thickness skin (2 × 2 cm) was excised to muscle fascia from the bilateral hind limbs of adult male Lewis rats. The graft/wound base boundary was identified by placement of a polypropylene mesh on the wound beneath the graft. Excised skin was replaced in its original orientation and secured with silk sutures tied over a gauze bolster dressing. After 3, 5, 7, and 10 days, animals were killed, and their aortas were cannulated and infused with an acrylic polymer to generate vascular casts. Grafts were excised, tissues were digested, and casts were examined with the use of scanning electron microscopy. Transmission electron microscopy was performed on tissues infused with the acrylic polymer that were not digested. At day 3, an immature lobular pattern was observed extending from the neovascular plexi on the graft side of the polypropylene mesh. At day 5, defined vessels with lobular ends occurred with high frequency. At day 7, the number of observed lobular structures was greatly reduced, and high frequencies of depressions in acrylic casts suggested protrusion of endothelial cell nuclei. By day 10, lobular structures were rare, well‐defined microvascular plexi were contiguous with larger vessels, and depressions from endothelial cell nuclei appeared more shallow and less frequent. These findings suggest that (1) an immature lobular pattern representing either capillary outgrowth or extracapillary leakage occurs at day 3; (2) these immature lobules decrease, and more discrete capillaries increase by day 5; (3) vascular integrity is reestablished by day 7; (4) vascular plexi has regained full continuity, and there are suggestions that endothelial cell proliferation has subsided by day 10.

UR - http://www.scopus.com/inward/record.url?scp=84989104577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989104577&partnerID=8YFLogxK

U2 - 10.1046/j.1524-475X.1995.30213.x

DO - 10.1046/j.1524-475X.1995.30213.x

M3 - Article

AN - SCOPUS:84989104577

VL - 3

SP - 213

EP - 220

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 2

ER -