Capacitation-like changes in equine spermatozoa following cryopreservation

A. D. Thomas, Stuart A Meyers, B. A. Ball

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The primary objective of this study was to assess plasma membrane characteristics and activation of signal transduction pathways in equine spermatozoa during both in vitro capacitation and cryopreservation. Significant plasma membrane restructuring, as assessed by measurement of plasma membrane lipid disorder and phospholipid scrambling, was not observed until after cryopreservation and subsequent thawing (P < 0.05). Although in vitro capacitated cells also displayed increased plasma membrane lipid disorder and phospholipid scrambling (P < 0.05), it appeared that regulation of these events in in vitro capacitated versus cryopreserved equine spermatozoa was not identical. Addition of 5 μM staurosporine to the capacitation media reduced plasma membrane phospholipid scrambling (P < 0.05), but supplementation to the freezing extender prior to cryopreservation did not. Furthermore, progesterone was able to induce a greater degree of acrosomal exocytosis in in vitro capacitated versus frozen/thawed spermatozoa. Expression of phospholipid scramblase, a protein thought to be important in plasma membrane phospholipid scrambling, did not differ between treatments. Comparison of protein tyrosine phosphorylation patterns between in vitro capacitated and cryopreserved cells demonstrated a divergence in signal transduction. Cellular signaling in in vitro capacitated equine spermatozoa appeared to be in part dependent on activation of the cAMP/PKA pathway, whereas signaling in cryopreserved cells seemed to proceed predominantly through alternative pathways. Taken together, these data support the idea that capacitation and "cryocapacitation" are not equivalent processes.

Original languageEnglish (US)
Pages (from-to)1531-1550
Number of pages20
JournalTheriogenology
Volume65
Issue number8
DOIs
StatePublished - May 2006

Fingerprint

Cryopreservation
cryopreservation
Horses
Spermatozoa
plasma membrane
spermatozoa
Cell Membrane
phospholipids
horses
Phospholipids
Membrane Lipids
signal transduction
Signal Transduction
Phospholipid Transfer Proteins
sperm capacitation
cell communication
Staurosporine
exocytosis
Exocytosis
cells

Keywords

  • Capacitation
  • Cryocapacitation
  • Cryopreservation
  • Equine
  • Sperm

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Capacitation-like changes in equine spermatozoa following cryopreservation. / Thomas, A. D.; Meyers, Stuart A; Ball, B. A.

In: Theriogenology, Vol. 65, No. 8, 05.2006, p. 1531-1550.

Research output: Contribution to journalArticle

Thomas, A. D. ; Meyers, Stuart A ; Ball, B. A. / Capacitation-like changes in equine spermatozoa following cryopreservation. In: Theriogenology. 2006 ; Vol. 65, No. 8. pp. 1531-1550.
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