Abstract
Canine CD4 + CD8α + double-positive (dp) T cells of peripheral blood are a unique effector memory T cell subpopulation characterized by an increased expression of activation markers in comparison with conventional CD4 + or CD8α + single-positive (sp) T cells. In this study, we investigated CD4 + CD8α + dp T cells in secondary lymphatic organs (i.e. mesenteric and tracheobronchial lymph nodes, spleen, Peyer’s patches) and non-lymphatic tissues (i.e. lung and epithelium of the small intestine) within a homogeneous group of healthy Beagle dogs by multi-color flow cytometry. The aim of this systematic analysis was to identify the tissue-specific localization and characteristics of this distinct T cell subpopulation. Our results revealed a mature extrathymic CD1a - CD4 + CD8α + dp T cell population in all analyzed organs, with highest frequencies within Peyer’s patches. Constitutive expression of the activation marker CD25 is a feature of many CD4 + CD8α + dp T cells independent of their localization and points to an effector phenotype. A proportion of lymph node CD4 + CD8α + dp T cells is FoxP3 + indicating regulatory potential. Within the intestinal environment, the cytotoxic marker granzyme B is expressed by CD4 + CD8α + dp intraepithelial lymphocytes. In addition, a fraction of CD4 + CD8α + dp intraepithelial lymphocytes and of mesenteric lymph node CD4 + CD8α + dp T cells is TCRγδ + . However, the main T cell receptor of all tissue-associated CD4 + CD8α + dp T cells could be identified as TCRαβ. Interestingly, the majority of the CD4 + CD8α + dp T cell subpopulation expresses the unconventional CD8αα homodimer, in contrast to CD8α + sp T cells, and CD4 + CD8α + dp thymocytes which are mainly CD8αβ + . The presented data provide the basis for a functional analysis of tissue-specific CD4 + CD8α + dp T cells to elucidate their role in health and disease of dogs.
| Original language | English (US) |
|---|---|
| Article number | e0213597 |
| Journal | PloS one |
| Volume | 14 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2019 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
Cite this
Canine tissue-associated CD4 + CD8α + double-positive T cells are an activated T cell subpopulation with heterogeneous functional potential . / Rabiger, Friederike V.; Bismarck, Doris; Protschka, Martina; Köhler, Gabriele; Moore, Peter F; Büttner, Mathias; Von Buttlar, Heiner; Alber, Gottfried; Eschke, Maria.
In: PloS one, Vol. 14, No. 3, e0213597, 01.03.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Canine tissue-associated CD4 + CD8α + double-positive T cells are an activated T cell subpopulation with heterogeneous functional potential
AU - Rabiger, Friederike V.
AU - Bismarck, Doris
AU - Protschka, Martina
AU - Köhler, Gabriele
AU - Moore, Peter F
AU - Büttner, Mathias
AU - Von Buttlar, Heiner
AU - Alber, Gottfried
AU - Eschke, Maria
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Canine CD4 + CD8α + double-positive (dp) T cells of peripheral blood are a unique effector memory T cell subpopulation characterized by an increased expression of activation markers in comparison with conventional CD4 + or CD8α + single-positive (sp) T cells. In this study, we investigated CD4 + CD8α + dp T cells in secondary lymphatic organs (i.e. mesenteric and tracheobronchial lymph nodes, spleen, Peyer’s patches) and non-lymphatic tissues (i.e. lung and epithelium of the small intestine) within a homogeneous group of healthy Beagle dogs by multi-color flow cytometry. The aim of this systematic analysis was to identify the tissue-specific localization and characteristics of this distinct T cell subpopulation. Our results revealed a mature extrathymic CD1a - CD4 + CD8α + dp T cell population in all analyzed organs, with highest frequencies within Peyer’s patches. Constitutive expression of the activation marker CD25 is a feature of many CD4 + CD8α + dp T cells independent of their localization and points to an effector phenotype. A proportion of lymph node CD4 + CD8α + dp T cells is FoxP3 + indicating regulatory potential. Within the intestinal environment, the cytotoxic marker granzyme B is expressed by CD4 + CD8α + dp intraepithelial lymphocytes. In addition, a fraction of CD4 + CD8α + dp intraepithelial lymphocytes and of mesenteric lymph node CD4 + CD8α + dp T cells is TCRγδ + . However, the main T cell receptor of all tissue-associated CD4 + CD8α + dp T cells could be identified as TCRαβ. Interestingly, the majority of the CD4 + CD8α + dp T cell subpopulation expresses the unconventional CD8αα homodimer, in contrast to CD8α + sp T cells, and CD4 + CD8α + dp thymocytes which are mainly CD8αβ + . The presented data provide the basis for a functional analysis of tissue-specific CD4 + CD8α + dp T cells to elucidate their role in health and disease of dogs.
AB - Canine CD4 + CD8α + double-positive (dp) T cells of peripheral blood are a unique effector memory T cell subpopulation characterized by an increased expression of activation markers in comparison with conventional CD4 + or CD8α + single-positive (sp) T cells. In this study, we investigated CD4 + CD8α + dp T cells in secondary lymphatic organs (i.e. mesenteric and tracheobronchial lymph nodes, spleen, Peyer’s patches) and non-lymphatic tissues (i.e. lung and epithelium of the small intestine) within a homogeneous group of healthy Beagle dogs by multi-color flow cytometry. The aim of this systematic analysis was to identify the tissue-specific localization and characteristics of this distinct T cell subpopulation. Our results revealed a mature extrathymic CD1a - CD4 + CD8α + dp T cell population in all analyzed organs, with highest frequencies within Peyer’s patches. Constitutive expression of the activation marker CD25 is a feature of many CD4 + CD8α + dp T cells independent of their localization and points to an effector phenotype. A proportion of lymph node CD4 + CD8α + dp T cells is FoxP3 + indicating regulatory potential. Within the intestinal environment, the cytotoxic marker granzyme B is expressed by CD4 + CD8α + dp intraepithelial lymphocytes. In addition, a fraction of CD4 + CD8α + dp intraepithelial lymphocytes and of mesenteric lymph node CD4 + CD8α + dp T cells is TCRγδ + . However, the main T cell receptor of all tissue-associated CD4 + CD8α + dp T cells could be identified as TCRαβ. Interestingly, the majority of the CD4 + CD8α + dp T cell subpopulation expresses the unconventional CD8αα homodimer, in contrast to CD8α + sp T cells, and CD4 + CD8α + dp thymocytes which are mainly CD8αβ + . The presented data provide the basis for a functional analysis of tissue-specific CD4 + CD8α + dp T cells to elucidate their role in health and disease of dogs.
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U2 - 10.1371/journal.pone.0213597
DO - 10.1371/journal.pone.0213597
M3 - Article
C2 - 30865691
AN - SCOPUS:85062885183
VL - 14
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 3
M1 - e0213597
ER -