TY - JOUR
T1 -
Canine tissue-associated CD4
+
CD8α
+
double-positive T cells are an activated T cell subpopulation with heterogeneous functional potential
AU - Rabiger, Friederike V.
AU - Bismarck, Doris
AU - Protschka, Martina
AU - Köhler, Gabriele
AU - Moore, Peter F
AU - Büttner, Mathias
AU - Von Buttlar, Heiner
AU - Alber, Gottfried
AU - Eschke, Maria
PY - 2019/3/1
Y1 - 2019/3/1
N2 -
Canine CD4
+
CD8α
+
double-positive (dp) T cells of peripheral blood are a unique effector memory T cell subpopulation characterized by an increased expression of activation markers in comparison with conventional CD4
+
or CD8α
+
single-positive (sp) T cells. In this study, we investigated CD4
+
CD8α
+
dp T cells in secondary lymphatic organs (i.e. mesenteric and tracheobronchial lymph nodes, spleen, Peyer’s patches) and non-lymphatic tissues (i.e. lung and epithelium of the small intestine) within a homogeneous group of healthy Beagle dogs by multi-color flow cytometry. The aim of this systematic analysis was to identify the tissue-specific localization and characteristics of this distinct T cell subpopulation. Our results revealed a mature extrathymic CD1a
-
CD4
+
CD8α
+
dp T cell population in all analyzed organs, with highest frequencies within Peyer’s patches. Constitutive expression of the activation marker CD25 is a feature of many CD4
+
CD8α
+
dp T cells independent of their localization and points to an effector phenotype. A proportion of lymph node CD4
+
CD8α
+
dp T cells is FoxP3
+
indicating regulatory potential. Within the intestinal environment, the cytotoxic marker granzyme B is expressed by CD4
+
CD8α
+
dp intraepithelial lymphocytes. In addition, a fraction of CD4
+
CD8α
+
dp intraepithelial lymphocytes and of mesenteric lymph node CD4
+
CD8α
+
dp T cells is TCRγδ
+
. However, the main T cell receptor of all tissue-associated CD4
+
CD8α
+
dp T cells could be identified as TCRαβ. Interestingly, the majority of the CD4
+
CD8α
+
dp T cell subpopulation expresses the unconventional CD8αα homodimer, in contrast to CD8α
+
sp T cells, and CD4
+
CD8α
+
dp thymocytes which are mainly CD8αβ
+
. The presented data provide the basis for a functional analysis of tissue-specific CD4
+
CD8α
+
dp T cells to elucidate their role in health and disease of dogs.
AB -
Canine CD4
+
CD8α
+
double-positive (dp) T cells of peripheral blood are a unique effector memory T cell subpopulation characterized by an increased expression of activation markers in comparison with conventional CD4
+
or CD8α
+
single-positive (sp) T cells. In this study, we investigated CD4
+
CD8α
+
dp T cells in secondary lymphatic organs (i.e. mesenteric and tracheobronchial lymph nodes, spleen, Peyer’s patches) and non-lymphatic tissues (i.e. lung and epithelium of the small intestine) within a homogeneous group of healthy Beagle dogs by multi-color flow cytometry. The aim of this systematic analysis was to identify the tissue-specific localization and characteristics of this distinct T cell subpopulation. Our results revealed a mature extrathymic CD1a
-
CD4
+
CD8α
+
dp T cell population in all analyzed organs, with highest frequencies within Peyer’s patches. Constitutive expression of the activation marker CD25 is a feature of many CD4
+
CD8α
+
dp T cells independent of their localization and points to an effector phenotype. A proportion of lymph node CD4
+
CD8α
+
dp T cells is FoxP3
+
indicating regulatory potential. Within the intestinal environment, the cytotoxic marker granzyme B is expressed by CD4
+
CD8α
+
dp intraepithelial lymphocytes. In addition, a fraction of CD4
+
CD8α
+
dp intraepithelial lymphocytes and of mesenteric lymph node CD4
+
CD8α
+
dp T cells is TCRγδ
+
. However, the main T cell receptor of all tissue-associated CD4
+
CD8α
+
dp T cells could be identified as TCRαβ. Interestingly, the majority of the CD4
+
CD8α
+
dp T cell subpopulation expresses the unconventional CD8αα homodimer, in contrast to CD8α
+
sp T cells, and CD4
+
CD8α
+
dp thymocytes which are mainly CD8αβ
+
. The presented data provide the basis for a functional analysis of tissue-specific CD4
+
CD8α
+
dp T cells to elucidate their role in health and disease of dogs.
UR - http://www.scopus.com/inward/record.url?scp=85062885183&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062885183&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0213597
DO - 10.1371/journal.pone.0213597
M3 - Article
C2 - 30865691
AN - SCOPUS:85062885183
VL - 14
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 3
M1 - e0213597
ER -