Canine spontaneous glioma: A translational model system for convection-enhanced delivery

Peter J Dickinson, Richard A Lecouteur, Robert Higgins, John R. Bringas, Richard F. Larson, Yoji Yamashita, Michal T. Krauze, John Forsayeth, Charles O. Noble, Daryl C. Drummond, Dmitri B. Kirpotin, John W. Park, Mitchel S. Berger, Krystof S. Bankiewicz

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Canine spontaneous intracranial tumors bear striking similarities to their human tumor counterparts and have the potential to provide a large animal model system for more realistic validation of novel therapies typically developed in small rodent models. We used spontaneously occurring canine gliomas to investigate the use of convection-enhanced delivery (CED) of liposomal nanoparticles, containing topoisomerase inhibitor CPT-11. To facilitate visualization of intratumoral infusions by real-time magnetic resonance imaging (MRI), we included identically formulated liposomes loaded with Gadoteridol. Real-time MRI defined distribution of infusate within both tumor and normal brain tissues. The most important limiting factor for volume of distribution within tumor tissue was the leakage of infusate into ventricular or subarachnoid spaces. Decreased tumor volume, tumor necrosis, and modulation of tumor phenotype correlated with volume of distribution of infusate (Vd), infusion location, and leakage as determined by real-time MRI and histopathology. This study demonstrates the potential for canine spontaneous gliomas as a model system for the validation and development of novel therapeutic strategies for human brain tumors. Data obtained from infusions monitored in real time in a large, spontaneous tumor may provide information, allowing more accurate prediction and optimization of infusion parameters. Variability in Vd between tumors strongly suggests that real-time imaging should be an essential component of CED therapeutic trials to allow minimization of inappropriate infusions and accurate assessment of clinical outcomes.

Original languageEnglish (US)
Pages (from-to)928-940
Number of pages13
JournalNeuro-Oncology
Volume12
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

Convection
Glioma
Canidae
Neoplasms
irinotecan
Magnetic Resonance Imaging
Brain Neoplasms
Topoisomerase Inhibitors
Subarachnoid Space
Tumor Burden
Liposomes
Nanoparticles
Rodentia
Necrosis
Therapeutics
Animal Models
Outcome Assessment (Health Care)
Phenotype

Keywords

  • Brain tumors
  • Canine
  • Convectionenhanced delivery
  • CPT-11
  • Magnetic resonance imaging

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

Cite this

Canine spontaneous glioma : A translational model system for convection-enhanced delivery. / Dickinson, Peter J; Lecouteur, Richard A; Higgins, Robert; Bringas, John R.; Larson, Richard F.; Yamashita, Yoji; Krauze, Michal T.; Forsayeth, John; Noble, Charles O.; Drummond, Daryl C.; Kirpotin, Dmitri B.; Park, John W.; Berger, Mitchel S.; Bankiewicz, Krystof S.

In: Neuro-Oncology, Vol. 12, No. 9, 09.2010, p. 928-940.

Research output: Contribution to journalArticle

Dickinson, PJ, Lecouteur, RA, Higgins, R, Bringas, JR, Larson, RF, Yamashita, Y, Krauze, MT, Forsayeth, J, Noble, CO, Drummond, DC, Kirpotin, DB, Park, JW, Berger, MS & Bankiewicz, KS 2010, 'Canine spontaneous glioma: A translational model system for convection-enhanced delivery', Neuro-Oncology, vol. 12, no. 9, pp. 928-940. https://doi.org/10.1093/neuonc/noq046
Dickinson, Peter J ; Lecouteur, Richard A ; Higgins, Robert ; Bringas, John R. ; Larson, Richard F. ; Yamashita, Yoji ; Krauze, Michal T. ; Forsayeth, John ; Noble, Charles O. ; Drummond, Daryl C. ; Kirpotin, Dmitri B. ; Park, John W. ; Berger, Mitchel S. ; Bankiewicz, Krystof S. / Canine spontaneous glioma : A translational model system for convection-enhanced delivery. In: Neuro-Oncology. 2010 ; Vol. 12, No. 9. pp. 928-940.
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