Canine lymphoma: Immunocytochemical analysis of fine-needle aspiration biopsy

M. Caniatti, P. Roccabianca, E. Scanziani, S. Paltrinieri, Peter F Moore

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68 Scopus citations


Cytospin preparations of fine-needle aspirates from 21 dogs with peripheral lymphadenopathy (18 with lymphoma and three with lymph node hyperplasia) were studied by combining morphologic and immunocytochemical analysis. Fine-needle aspirates were taken from at least two enlarged lymph nodes, and the diagnosis was based on air-dried smears stained with May-Grünwald Giemsa. Fine-needle aspiration biopsy always provided an adequate quality and quantity of cells to perform morphologic and immunologic studies. Immunophenotyping was performed on cytospin preparations with a panel of eight monoclonal antibodies specific for canine cell surface antigens and one rabbit polyclonal antibody (A452) against human CD3, which cross-reacts with dog antigen. The immunocytochemical study resulted in the diagnosis of 14 B-cell lymphomas (CD21+, CD3-) and three T-cell lymphomas (all CD3+, two CD8+). One lymphoma lacked surface antigens specific for the B- or T-cell lineage and was classified as non-B-non-T lymphoma (CD21-, CD3-, CD4-, CD8-). The monoclonal antibodies CA12.10C12, CA4.1D3, and CA1D6 and the polyclonal antibody A452, used as a group, appeared to be the most useful reagents to suggest lymphoid origin and to discriminate between T- and B-cell phenotype. Cytospin preparations in combination with immunocytochemistry provided a practical, economical, and accurate method for the diagnosis and phenotyping of canine lymphoma.

Original languageEnglish (US)
Pages (from-to)204-212
Number of pages9
JournalVeterinary Pathology
Issue number2
StatePublished - Mar 1996


  • Cytology
  • Dogs
  • Fine-needle aspiration
  • Immunocytochemistry
  • Lymphoma
  • Neoplasm

ASJC Scopus subject areas

  • veterinary(all)


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