Canine lymphoma

Immunocytochemical analysis of fine-needle aspiration biopsy

M. Caniatti, P. Roccabianca, E. Scanziani, S. Paltrinieri, Peter F Moore

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Cytospin preparations of fine-needle aspirates from 21 dogs with peripheral lymphadenopathy (18 with lymphoma and three with lymph node hyperplasia) were studied by combining morphologic and immunocytochemical analysis. Fine-needle aspirates were taken from at least two enlarged lymph nodes, and the diagnosis was based on air-dried smears stained with May-Grünwald Giemsa. Fine-needle aspiration biopsy always provided an adequate quality and quantity of cells to perform morphologic and immunologic studies. Immunophenotyping was performed on cytospin preparations with a panel of eight monoclonal antibodies specific for canine cell surface antigens and one rabbit polyclonal antibody (A452) against human CD3, which cross-reacts with dog antigen. The immunocytochemical study resulted in the diagnosis of 14 B-cell lymphomas (CD21+, CD3-) and three T-cell lymphomas (all CD3+, two CD8+). One lymphoma lacked surface antigens specific for the B- or T-cell lineage and was classified as non-B-non-T lymphoma (CD21-, CD3-, CD4-, CD8-). The monoclonal antibodies CA12.10C12, CA4.1D3, and CA1D6 and the polyclonal antibody A452, used as a group, appeared to be the most useful reagents to suggest lymphoid origin and to discriminate between T- and B-cell phenotype. Cytospin preparations in combination with immunocytochemistry provided a practical, economical, and accurate method for the diagnosis and phenotyping of canine lymphoma.

Original languageEnglish (US)
Pages (from-to)204-212
Number of pages9
JournalVeterinary Pathology
Volume33
Issue number2
StatePublished - Mar 1996

Fingerprint

Fine Needle Biopsy
lymphoma
Canidae
biopsy
Lymphoma
dogs
Surface Antigens
B-lymphocytes
Needles
T-lymphocytes
Lymph Nodes
Monoclonal Antibodies
surface antigens
Dogs
polyclonal antibodies
Immunophenotyping
lymph nodes
Antibodies
T-Cell Lymphoma
monoclonal antibodies

Keywords

  • Cytology
  • Dogs
  • Fine-needle aspiration
  • Immunocytochemistry
  • Lymphoma
  • Neoplasm

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Caniatti, M., Roccabianca, P., Scanziani, E., Paltrinieri, S., & Moore, P. F. (1996). Canine lymphoma: Immunocytochemical analysis of fine-needle aspiration biopsy. Veterinary Pathology, 33(2), 204-212.

Canine lymphoma : Immunocytochemical analysis of fine-needle aspiration biopsy. / Caniatti, M.; Roccabianca, P.; Scanziani, E.; Paltrinieri, S.; Moore, Peter F.

In: Veterinary Pathology, Vol. 33, No. 2, 03.1996, p. 204-212.

Research output: Contribution to journalArticle

Caniatti, M, Roccabianca, P, Scanziani, E, Paltrinieri, S & Moore, PF 1996, 'Canine lymphoma: Immunocytochemical analysis of fine-needle aspiration biopsy', Veterinary Pathology, vol. 33, no. 2, pp. 204-212.
Caniatti M, Roccabianca P, Scanziani E, Paltrinieri S, Moore PF. Canine lymphoma: Immunocytochemical analysis of fine-needle aspiration biopsy. Veterinary Pathology. 1996 Mar;33(2):204-212.
Caniatti, M. ; Roccabianca, P. ; Scanziani, E. ; Paltrinieri, S. ; Moore, Peter F. / Canine lymphoma : Immunocytochemical analysis of fine-needle aspiration biopsy. In: Veterinary Pathology. 1996 ; Vol. 33, No. 2. pp. 204-212.
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