Canine keratinocytes upregulate type I interferons and proinflammatory cytokines in response to poly(dA

DT) but not to canine papillomavirus

Jennifer A. Luff, Hang Yuan, Maja M. Suter, Eliane J. Müller, Richard Schlegel, Peter F Moore

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Papillomaviruses (PV) are double stranded (ds) DNA viruses that infect epithelial cells within the skin or mucosa, most often causing benign neoplasms that spontaneously regress. The immune system plays a key role in the defense against PVs. Since these viruses infect keratinocytes, we wanted to investigate the role of the keratinocyte in initiating an immune response to canine papillomavirus-2 (CPV-2) in the dog. Keratinocytes express a variety of pattern recognition receptors (PRR) to distinguish different cutaneous pathogens and initiate an immune response. We examined the mRNA expression patterns for several recently described cytosolic nucleic acid sensing PRRs in canine monolayer keratinocyte cultures using quantitative reverse transcription-polymerase chain reaction. Unstimulated normal cells were found to express mRNA for melanoma differentiation associated gene 5 (MDA5), retinoic acid-inducible gene I (RIG-I), DNA-dependent activation of interferon regulatory factors, leucine rich repeat flightless interacting protein 1, and interferon inducible gene 16 (IFI16), as well as their adaptor molecules myeloid differentiation primary response gene 88, interferon-β promoter stimulator 1, and endoplasmic reticulum-resident transmembrane protein stimulator of interferon genes. When stimulated with synthetic dsDNA [poly(dA:dT)] or dsRNA [poly(I:C)], keratinocytes responded with increased mRNA expression levels for interleukin-6, tumor necrosis factor-α, interferon-β, RIG-I, IFI16, and MDA5. There was no detectable increase in mRNA expression, however, in keratinocytes infected with CPV-2. Furthermore, CPV-2-infected keratinocytes stimulated with poly(dA:dT) and poly(I:C) showed similar mRNA expression levels for these gene products when compared with expression levels in uninfected cells. These results suggest that although canine keratinocytes contain functional PRRs that can recognize and respond to dsDNA and dsRNA ligands, they do not appear to recognize or initiate a similar response to CPV-2.

Original languageEnglish (US)
Pages (from-to)177-186
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume153
Issue number3-4
DOIs
StatePublished - Jun 15 2013

Fingerprint

Interferon Type I
Papillomaviridae
keratinocytes
interferons
Keratinocytes
Canidae
cytokines
Up-Regulation
Cytokines
Interferons
dogs
Genes
genes
Messenger RNA
Poly dA-dT
polyinosinic-polycytidylic acid
Tretinoin
retinoic acid
double-stranded RNA
melanoma

Keywords

  • Canine papillomavirus
  • Keratinocyte
  • Pattern recognition receptors

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

Canine keratinocytes upregulate type I interferons and proinflammatory cytokines in response to poly(dA : DT) but not to canine papillomavirus. / Luff, Jennifer A.; Yuan, Hang; Suter, Maja M.; Müller, Eliane J.; Schlegel, Richard; Moore, Peter F.

In: Veterinary Immunology and Immunopathology, Vol. 153, No. 3-4, 15.06.2013, p. 177-186.

Research output: Contribution to journalArticle

Luff, Jennifer A. ; Yuan, Hang ; Suter, Maja M. ; Müller, Eliane J. ; Schlegel, Richard ; Moore, Peter F. / Canine keratinocytes upregulate type I interferons and proinflammatory cytokines in response to poly(dA : DT) but not to canine papillomavirus. In: Veterinary Immunology and Immunopathology. 2013 ; Vol. 153, No. 3-4. pp. 177-186.
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