Canine gut dendritic cells in the steady state and in inflammatory bowel disease

Johannes Junginger, Frederik Lemensieck, Peter F Moore, Ulrike Schwittlick, Ingo Nolte, Marion Hewicker-Trautwein

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Alongside the intestinal border, dendritic cells (DCs) sample large amounts of endogenous and potentially pathogenic antigens followed by initiation of protective immune responses or induction of tolerance. Breakdown of oral tolerance towards commensal bacteria is suggested to be crucial for the development of both human and canine inflammatory bowel disease (IBD). The aim of this study was to investigate canine intestinal DCs in the steady state and in dogs with IBD using multicolour immunofluorescence. In the healthy gut, DC-like cells expressed MHC II, CD1a8.2 and CD11c, and, in lower amounts, CD11b, within lamina propria, Peyer's patches (PPs) and mesenteric lymph nodes (MLNs), whereas those expressing CD80 and CD86 were only present in PPs and MLNs. Occasionally, DC-like cells were in contact with the intestinal lumen through transepithelial projections. In canine IBD, CD1a8.2+, CD11b+ and CD11c+ DC-like cells were decreased within the stomach, duodenum and colon, whereas the colonic mucosa revealed elevation of CD86+ DC-like cells. The complex network of DC-like cells in the gut indicates their important role in canine mucosal immunity, including active sampling of luminal antigens. Furthermore, their shift in diseased dogs suggests a pathogenetic significance for canine IBD.

Original languageEnglish (US)
Pages (from-to)145-160
Number of pages16
JournalInnate Immunity
Volume20
Issue number2
DOIs
StatePublished - Feb 2014

Fingerprint

Inflammatory Bowel Diseases
Dendritic Cells
Canidae
Peyer's Patches
Mucous Membrane
Lymph Nodes
Dog Diseases
Antigens
Mucosal Immunity
Human Development
Duodenum
Fluorescent Antibody Technique
Stomach
Colon
Dogs
Bacteria

Keywords

  • DC
  • Dog
  • enteropathy
  • gastrointestinal
  • IBD

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

Junginger, J., Lemensieck, F., Moore, P. F., Schwittlick, U., Nolte, I., & Hewicker-Trautwein, M. (2014). Canine gut dendritic cells in the steady state and in inflammatory bowel disease. Innate Immunity, 20(2), 145-160. https://doi.org/10.1177/1753425913485475

Canine gut dendritic cells in the steady state and in inflammatory bowel disease. / Junginger, Johannes; Lemensieck, Frederik; Moore, Peter F; Schwittlick, Ulrike; Nolte, Ingo; Hewicker-Trautwein, Marion.

In: Innate Immunity, Vol. 20, No. 2, 02.2014, p. 145-160.

Research output: Contribution to journalArticle

Junginger, J, Lemensieck, F, Moore, PF, Schwittlick, U, Nolte, I & Hewicker-Trautwein, M 2014, 'Canine gut dendritic cells in the steady state and in inflammatory bowel disease', Innate Immunity, vol. 20, no. 2, pp. 145-160. https://doi.org/10.1177/1753425913485475
Junginger J, Lemensieck F, Moore PF, Schwittlick U, Nolte I, Hewicker-Trautwein M. Canine gut dendritic cells in the steady state and in inflammatory bowel disease. Innate Immunity. 2014 Feb;20(2):145-160. https://doi.org/10.1177/1753425913485475
Junginger, Johannes ; Lemensieck, Frederik ; Moore, Peter F ; Schwittlick, Ulrike ; Nolte, Ingo ; Hewicker-Trautwein, Marion. / Canine gut dendritic cells in the steady state and in inflammatory bowel disease. In: Innate Immunity. 2014 ; Vol. 20, No. 2. pp. 145-160.
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