Abstract
Alongside the intestinal border, dendritic cells (DCs) sample large amounts of endogenous and potentially pathogenic antigens followed by initiation of protective immune responses or induction of tolerance. Breakdown of oral tolerance towards commensal bacteria is suggested to be crucial for the development of both human and canine inflammatory bowel disease (IBD). The aim of this study was to investigate canine intestinal DCs in the steady state and in dogs with IBD using multicolour immunofluorescence. In the healthy gut, DC-like cells expressed MHC II, CD1a8.2 and CD11c, and, in lower amounts, CD11b, within lamina propria, Peyer's patches (PPs) and mesenteric lymph nodes (MLNs), whereas those expressing CD80 and CD86 were only present in PPs and MLNs. Occasionally, DC-like cells were in contact with the intestinal lumen through transepithelial projections. In canine IBD, CD1a8.2+, CD11b+ and CD11c+ DC-like cells were decreased within the stomach, duodenum and colon, whereas the colonic mucosa revealed elevation of CD86+ DC-like cells. The complex network of DC-like cells in the gut indicates their important role in canine mucosal immunity, including active sampling of luminal antigens. Furthermore, their shift in diseased dogs suggests a pathogenetic significance for canine IBD.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-160 |
| Number of pages | 16 |
| Journal | Innate Immunity |
| Volume | 20 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2014 |
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Keywords
- DC
- Dog
- enteropathy
- gastrointestinal
- IBD
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Immunology
- Microbiology
- Infectious Diseases
Cite this
Canine gut dendritic cells in the steady state and in inflammatory bowel disease. / Junginger, Johannes; Lemensieck, Frederik; Moore, Peter F; Schwittlick, Ulrike; Nolte, Ingo; Hewicker-Trautwein, Marion.
In: Innate Immunity, Vol. 20, No. 2, 02.2014, p. 145-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Canine gut dendritic cells in the steady state and in inflammatory bowel disease
AU - Junginger, Johannes
AU - Lemensieck, Frederik
AU - Moore, Peter F
AU - Schwittlick, Ulrike
AU - Nolte, Ingo
AU - Hewicker-Trautwein, Marion
PY - 2014/2
Y1 - 2014/2
N2 - Alongside the intestinal border, dendritic cells (DCs) sample large amounts of endogenous and potentially pathogenic antigens followed by initiation of protective immune responses or induction of tolerance. Breakdown of oral tolerance towards commensal bacteria is suggested to be crucial for the development of both human and canine inflammatory bowel disease (IBD). The aim of this study was to investigate canine intestinal DCs in the steady state and in dogs with IBD using multicolour immunofluorescence. In the healthy gut, DC-like cells expressed MHC II, CD1a8.2 and CD11c, and, in lower amounts, CD11b, within lamina propria, Peyer's patches (PPs) and mesenteric lymph nodes (MLNs), whereas those expressing CD80 and CD86 were only present in PPs and MLNs. Occasionally, DC-like cells were in contact with the intestinal lumen through transepithelial projections. In canine IBD, CD1a8.2+, CD11b+ and CD11c+ DC-like cells were decreased within the stomach, duodenum and colon, whereas the colonic mucosa revealed elevation of CD86+ DC-like cells. The complex network of DC-like cells in the gut indicates their important role in canine mucosal immunity, including active sampling of luminal antigens. Furthermore, their shift in diseased dogs suggests a pathogenetic significance for canine IBD.
AB - Alongside the intestinal border, dendritic cells (DCs) sample large amounts of endogenous and potentially pathogenic antigens followed by initiation of protective immune responses or induction of tolerance. Breakdown of oral tolerance towards commensal bacteria is suggested to be crucial for the development of both human and canine inflammatory bowel disease (IBD). The aim of this study was to investigate canine intestinal DCs in the steady state and in dogs with IBD using multicolour immunofluorescence. In the healthy gut, DC-like cells expressed MHC II, CD1a8.2 and CD11c, and, in lower amounts, CD11b, within lamina propria, Peyer's patches (PPs) and mesenteric lymph nodes (MLNs), whereas those expressing CD80 and CD86 were only present in PPs and MLNs. Occasionally, DC-like cells were in contact with the intestinal lumen through transepithelial projections. In canine IBD, CD1a8.2+, CD11b+ and CD11c+ DC-like cells were decreased within the stomach, duodenum and colon, whereas the colonic mucosa revealed elevation of CD86+ DC-like cells. The complex network of DC-like cells in the gut indicates their important role in canine mucosal immunity, including active sampling of luminal antigens. Furthermore, their shift in diseased dogs suggests a pathogenetic significance for canine IBD.
KW - DC
KW - Dog
KW - enteropathy
KW - gastrointestinal
KW - IBD
UR - http://www.scopus.com/inward/record.url?scp=84892742389&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84892742389&partnerID=8YFLogxK
U2 - 10.1177/1753425913485475
DO - 10.1177/1753425913485475
M3 - Article
C2 - 23723379
AN - SCOPUS:84892742389
VL - 20
SP - 145
EP - 160
JO - Innate Immunity
JF - Innate Immunity
SN - 1753-4259
IS - 2
ER -