Canine COL4A3 and COL4A4: Sequencing, mapping and genomic organization

Anje C. Wiersma, Lee V. Millon, Matthew S. Hestand, Bernard A. Van Oost, Danika L Bannasch

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Canine α3 and α4 chains of collagen type IV genes (COL4A3 and COL4A4) are expressed in the renal glomerular basement membrane, where they provide a critical structural and functional matrix for other basement membrane components. These genes are candidates for hereditary nephritis (Alport syndrome) in several dog breeds (e.g. English Cocker Spaniel and Bull Terrier). Using RACE and PCR, the cDNA of both genes was cloned and sequenced. Both COL4A3 and COL4A4, as well as canine NPPC (Natriuretic Peptide Precursor C), were mapped to CFA25 using an RH panel. Conservation of the tight linkage of COL4A3 and COL4A4 as seen in human and mouse was verified in the dog. Intron-exon boundaries in both genes were determined by BLAST analysis of the Canis Familiaris Trace Archive. The elucidation of the cDNA sequences, genomic organization and the open reading frames of canine COL4A3 and COL4A4 provide the groundwork for screening these genes for mutations in hereditary nephritis in dogs.

Original languageEnglish (US)
Pages (from-to)241-251
Number of pages11
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume16
Issue number4
DOIs
StatePublished - Aug 2005

Fingerprint

Canidae
Hereditary Nephritis
Genes
Dogs
Complementary DNA
Glomerular Basement Membrane
Natriuretic Peptides
Collagen Type IV
Basement Membrane
Introns
Open Reading Frames
Exons
Conservation
Screening
Kidney
Polymerase Chain Reaction
Mutation

Keywords

  • Alport syndrome
  • Canine hereditary nephritis
  • Collagen chains
  • Collagen type IV

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Canine COL4A3 and COL4A4 : Sequencing, mapping and genomic organization. / Wiersma, Anje C.; Millon, Lee V.; Hestand, Matthew S.; Van Oost, Bernard A.; Bannasch, Danika L.

In: DNA Sequence - Journal of DNA Sequencing and Mapping, Vol. 16, No. 4, 08.2005, p. 241-251.

Research output: Contribution to journalArticle

Wiersma, Anje C. ; Millon, Lee V. ; Hestand, Matthew S. ; Van Oost, Bernard A. ; Bannasch, Danika L. / Canine COL4A3 and COL4A4 : Sequencing, mapping and genomic organization. In: DNA Sequence - Journal of DNA Sequencing and Mapping. 2005 ; Vol. 16, No. 4. pp. 241-251.
@article{283209b513f94ed1a6d61b7ab5017904,
title = "Canine COL4A3 and COL4A4: Sequencing, mapping and genomic organization",
abstract = "Canine α3 and α4 chains of collagen type IV genes (COL4A3 and COL4A4) are expressed in the renal glomerular basement membrane, where they provide a critical structural and functional matrix for other basement membrane components. These genes are candidates for hereditary nephritis (Alport syndrome) in several dog breeds (e.g. English Cocker Spaniel and Bull Terrier). Using RACE and PCR, the cDNA of both genes was cloned and sequenced. Both COL4A3 and COL4A4, as well as canine NPPC (Natriuretic Peptide Precursor C), were mapped to CFA25 using an RH panel. Conservation of the tight linkage of COL4A3 and COL4A4 as seen in human and mouse was verified in the dog. Intron-exon boundaries in both genes were determined by BLAST analysis of the Canis Familiaris Trace Archive. The elucidation of the cDNA sequences, genomic organization and the open reading frames of canine COL4A3 and COL4A4 provide the groundwork for screening these genes for mutations in hereditary nephritis in dogs.",
keywords = "Alport syndrome, Canine hereditary nephritis, Collagen chains, Collagen type IV",
author = "Wiersma, {Anje C.} and Millon, {Lee V.} and Hestand, {Matthew S.} and {Van Oost}, {Bernard A.} and Bannasch, {Danika L}",
year = "2005",
month = "8",
doi = "10.1080/10425170500136822",
language = "English (US)",
volume = "16",
pages = "241--251",
journal = "DNA Sequence - Journal of DNA Sequencing and Mapping",
issn = "1940-1736",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Canine COL4A3 and COL4A4

T2 - Sequencing, mapping and genomic organization

AU - Wiersma, Anje C.

AU - Millon, Lee V.

AU - Hestand, Matthew S.

AU - Van Oost, Bernard A.

AU - Bannasch, Danika L

PY - 2005/8

Y1 - 2005/8

N2 - Canine α3 and α4 chains of collagen type IV genes (COL4A3 and COL4A4) are expressed in the renal glomerular basement membrane, where they provide a critical structural and functional matrix for other basement membrane components. These genes are candidates for hereditary nephritis (Alport syndrome) in several dog breeds (e.g. English Cocker Spaniel and Bull Terrier). Using RACE and PCR, the cDNA of both genes was cloned and sequenced. Both COL4A3 and COL4A4, as well as canine NPPC (Natriuretic Peptide Precursor C), were mapped to CFA25 using an RH panel. Conservation of the tight linkage of COL4A3 and COL4A4 as seen in human and mouse was verified in the dog. Intron-exon boundaries in both genes were determined by BLAST analysis of the Canis Familiaris Trace Archive. The elucidation of the cDNA sequences, genomic organization and the open reading frames of canine COL4A3 and COL4A4 provide the groundwork for screening these genes for mutations in hereditary nephritis in dogs.

AB - Canine α3 and α4 chains of collagen type IV genes (COL4A3 and COL4A4) are expressed in the renal glomerular basement membrane, where they provide a critical structural and functional matrix for other basement membrane components. These genes are candidates for hereditary nephritis (Alport syndrome) in several dog breeds (e.g. English Cocker Spaniel and Bull Terrier). Using RACE and PCR, the cDNA of both genes was cloned and sequenced. Both COL4A3 and COL4A4, as well as canine NPPC (Natriuretic Peptide Precursor C), were mapped to CFA25 using an RH panel. Conservation of the tight linkage of COL4A3 and COL4A4 as seen in human and mouse was verified in the dog. Intron-exon boundaries in both genes were determined by BLAST analysis of the Canis Familiaris Trace Archive. The elucidation of the cDNA sequences, genomic organization and the open reading frames of canine COL4A3 and COL4A4 provide the groundwork for screening these genes for mutations in hereditary nephritis in dogs.

KW - Alport syndrome

KW - Canine hereditary nephritis

KW - Collagen chains

KW - Collagen type IV

UR - http://www.scopus.com/inward/record.url?scp=27844547759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27844547759&partnerID=8YFLogxK

U2 - 10.1080/10425170500136822

DO - 10.1080/10425170500136822

M3 - Article

C2 - 16147883

AN - SCOPUS:27844547759

VL - 16

SP - 241

EP - 251

JO - DNA Sequence - Journal of DNA Sequencing and Mapping

JF - DNA Sequence - Journal of DNA Sequencing and Mapping

SN - 1940-1736

IS - 4

ER -