Abstract
Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1743-1750 |
| Number of pages | 8 |
| Journal | Anticancer Research |
| Volume | 9 |
| Issue number | 6 |
| State | Published - 1989 |
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Keywords
- bovine
- canine
- PCR
- polymerase chain reaction
- proto-oncogenes
- RAS
- RNA/PCR
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction. / Madewell BG., R.; Gumerlock, P. H.; Saunders, K. A.; Meyers, Frederick J; Kraegel, S. A.; Kawasaki, E. S.
In: Anticancer Research, Vol. 9, No. 6, 1989, p. 1743-1750.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction
AU - Madewell BG., R.
AU - Gumerlock, P. H.
AU - Saunders, K. A.
AU - Meyers, Frederick J
AU - Kraegel, S. A.
AU - Kawasaki, E. S.
PY - 1989
Y1 - 1989
N2 - Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).
AB - Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).
KW - bovine
KW - canine
KW - PCR
KW - polymerase chain reaction
KW - proto-oncogenes
KW - RAS
KW - RNA/PCR
UR - http://www.scopus.com/inward/record.url?scp=0024803899&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024803899&partnerID=8YFLogxK
M3 - Article
C2 - 2483304
AN - SCOPUS:0024803899
VL - 9
SP - 1743
EP - 1750
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 6
ER -