Abstract
Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).
Original language | English (US) |
---|---|
Pages (from-to) | 1743-1750 |
Number of pages | 8 |
Journal | Anticancer Research |
Volume | 9 |
Issue number | 6 |
State | Published - 1989 |
Keywords
- bovine
- canine
- PCR
- polymerase chain reaction
- proto-oncogenes
- RAS
- RNA/PCR
ASJC Scopus subject areas
- Cancer Research
- Oncology