Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction

R. Madewell BG., P. H. Gumerlock, K. A. Saunders, Frederick J Meyers, S. A. Kraegel, E. S. Kawasaki

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).

Original languageEnglish (US)
Pages (from-to)1743-1750
Number of pages8
JournalAnticancer Research
Volume9
Issue number6
StatePublished - 1989

Keywords

  • bovine
  • canine
  • PCR
  • polymerase chain reaction
  • proto-oncogenes
  • RAS
  • RNA/PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Madewell BG., R., Gumerlock, P. H., Saunders, K. A., Meyers, F. J., Kraegel, S. A., & Kawasaki, E. S. (1989). Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction. Anticancer Research, 9(6), 1743-1750.