Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction

R. Madewell BG., P. H. Gumerlock, K. A. Saunders, Frederick J Meyers, S. A. Kraegel, E. S. Kawasaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).

Original languageEnglish (US)
Pages (from-to)1743-1750
Number of pages8
JournalAnticancer Research
Volume9
Issue number6
StatePublished - 1989

Fingerprint

Canidae
Polymerase Chain Reaction
DNA Primers
ras Genes
Proto-Oncogenes
DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Genes
Cell Differentiation
Dogs
Messenger RNA
DNA
Growth

Keywords

  • bovine
  • canine
  • PCR
  • polymerase chain reaction
  • proto-oncogenes
  • RAS
  • RNA/PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Madewell BG., R., Gumerlock, P. H., Saunders, K. A., Meyers, F. J., Kraegel, S. A., & Kawasaki, E. S. (1989). Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction. Anticancer Research, 9(6), 1743-1750.

Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction. / Madewell BG., R.; Gumerlock, P. H.; Saunders, K. A.; Meyers, Frederick J; Kraegel, S. A.; Kawasaki, E. S.

In: Anticancer Research, Vol. 9, No. 6, 1989, p. 1743-1750.

Research output: Contribution to journalArticle

Madewell BG., R, Gumerlock, PH, Saunders, KA, Meyers, FJ, Kraegel, SA & Kawasaki, ES 1989, 'Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction', Anticancer Research, vol. 9, no. 6, pp. 1743-1750.
Madewell BG. R, Gumerlock PH, Saunders KA, Meyers FJ, Kraegel SA, Kawasaki ES. Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction. Anticancer Research. 1989;9(6):1743-1750.
Madewell BG., R. ; Gumerlock, P. H. ; Saunders, K. A. ; Meyers, Frederick J ; Kraegel, S. A. ; Kawasaki, E. S. / Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction. In: Anticancer Research. 1989 ; Vol. 9, No. 6. pp. 1743-1750.
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