Abstract
Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1743-1750 |
| Number of pages | 8 |
| Journal | Anticancer Research |
| Volume | 9 |
| Issue number | 6 |
| State | Published - 1989 |
Keywords
- bovine
- canine
- PCR
- polymerase chain reaction
- proto-oncogenes
- RAS
- RNA/PCR
ASJC Scopus subject areas
- Cancer Research
- Oncology
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Madewell BG., R., Gumerlock, P. H., Saunders, K. A., Meyers, F. J., Kraegel, S. A., & Kawasaki, E. S. (1989). Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction. Anticancer Research, 9(6), 1743-1750.