Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction

R. Madewell BG.; P. H. Gumerlock; K. A. Saunders; Frederick J Meyers; S. A. Kraegel; E. S. Kawasaki

Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction

R. Madewell BG., P. H. Gumerlock, K. A. Saunders, Frederick J Meyers, S. A. Kraegel, E. S. Kawasaki

Hematology and Oncology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).

Original language	English (US)
Pages (from-to)	1743-1750
Number of pages	8
Journal	Anticancer Research
Volume	9
Issue number	6
State	Published - 1989

Keywords

bovine
canine
PCR
polymerase chain reaction
proto-oncogenes
RAS
RNA/PCR

ASJC Scopus subject areas

Cancer Research
Oncology

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title = "Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction",

abstract = "Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).",

keywords = "bovine, canine, PCR, polymerase chain reaction, proto-oncogenes, RAS, RNA/PCR",

author = "{Madewell BG.}, R. and Gumerlock, {P. H.} and Saunders, {K. A.} and Meyers, {Frederick J} and Kraegel, {S. A.} and Kawasaki, {E. S.}",

year = "1989",

language = "English (US)",

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pages = "1743--1750",

journal = "Anticancer Research",

issn = "0250-7005",

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T1 - Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction

AU - Madewell BG., R.

AU - Gumerlock, P. H.

AU - Saunders, K. A.

AU - Meyers, Frederick J

AU - Kraegel, S. A.

AU - Kawasaki, E. S.

PY - 1989

Y1 - 1989

N2 - Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).

AB - Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).

KW - bovine

KW - canine

KW - PCR

KW - polymerase chain reaction

KW - proto-oncogenes

KW - RAS

KW - RNA/PCR

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SP - 1743

EP - 1750

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 6

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