Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues

Cristina Parrado-Fernández, Guillermo López-Lluch, Elisabet Rodríguez-Bies, Sara Santa-Cruz, Plácido Navas, Jon J Ramsey, José M. Villalba

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We studied ubiquinone (Q), Q homologue ratio, and steady-state levels of mCOQ transcripts in tissues from mice fed ad libitum or under calorie restriction. Maximum ubiquinone levels on a protein basis were found in kidney and heart, followed by liver, brain, and skeletal muscle. Liver and skeletal muscle showed the highest Q9/Q10 ratios with significant interindividual variability. Heart, kidney, and particularly brain exhibited lower Q9/Q10 ratios and interindividual variability. In skeletal muscle and heart, the most abundant mCOQ transcript was mCOQ7, followed by mCOQ8, mCOQ2, mPDSS2, mPDSS1, and mCOQ3. In nonmuscular tissues (liver, kidney, and brain) the most abundant mCOQ transcript was mCOQ2, followed by mCOQ7, mCOQ8, mPDSS1, mPDSS2, and mCOQ3. Calorie restriction increased both ubiquinone homologues and mPDSS2 mRNA in skeletal muscle, but mCOQ7 was decreased. In contrast, Q9 and most mCOQ transcripts were decreased in heart. Calorie restriction also modified the Q9/Q10 ratio, which was increased in kidney and decreased in heart without alterations in mPDSS1 or mPDSS2 transcripts. We demonstrate for the first time that unique patterns of mCOQ transcripts exist in muscular and nonmuscular tissues and that Q and COQ genes are targets of calorie restriction in a tissue-specific way.

Original languageEnglish (US)
Pages (from-to)1728-1736
Number of pages9
JournalFree Radical Biology and Medicine
Volume50
Issue number12
DOIs
StatePublished - Jun 15 2011

Fingerprint

Ubiquinone
Muscle
Tissue
Skeletal Muscle
Liver
Kidney
Brain
vif Genes
Genes
Messenger RNA
Proteins

Keywords

  • Calorie restriction
  • COQ transcripts
  • Free radicals
  • Ubiquinone
  • Ubiquinone biosynthesis

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Parrado-Fernández, C., López-Lluch, G., Rodríguez-Bies, E., Santa-Cruz, S., Navas, P., Ramsey, J. J., & Villalba, J. M. (2011). Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues. Free Radical Biology and Medicine, 50(12), 1728-1736. https://doi.org/10.1016/j.freeradbiomed.2011.03.024

Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues. / Parrado-Fernández, Cristina; López-Lluch, Guillermo; Rodríguez-Bies, Elisabet; Santa-Cruz, Sara; Navas, Plácido; Ramsey, Jon J; Villalba, José M.

In: Free Radical Biology and Medicine, Vol. 50, No. 12, 15.06.2011, p. 1728-1736.

Research output: Contribution to journalArticle

Parrado-Fernández, C, López-Lluch, G, Rodríguez-Bies, E, Santa-Cruz, S, Navas, P, Ramsey, JJ & Villalba, JM 2011, 'Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues', Free Radical Biology and Medicine, vol. 50, no. 12, pp. 1728-1736. https://doi.org/10.1016/j.freeradbiomed.2011.03.024
Parrado-Fernández C, López-Lluch G, Rodríguez-Bies E, Santa-Cruz S, Navas P, Ramsey JJ et al. Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues. Free Radical Biology and Medicine. 2011 Jun 15;50(12):1728-1736. https://doi.org/10.1016/j.freeradbiomed.2011.03.024
Parrado-Fernández, Cristina ; López-Lluch, Guillermo ; Rodríguez-Bies, Elisabet ; Santa-Cruz, Sara ; Navas, Plácido ; Ramsey, Jon J ; Villalba, José M. / Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues. In: Free Radical Biology and Medicine. 2011 ; Vol. 50, No. 12. pp. 1728-1736.
@article{87d4e0060e314e94ae34db6e56575542,
title = "Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues",
abstract = "We studied ubiquinone (Q), Q homologue ratio, and steady-state levels of mCOQ transcripts in tissues from mice fed ad libitum or under calorie restriction. Maximum ubiquinone levels on a protein basis were found in kidney and heart, followed by liver, brain, and skeletal muscle. Liver and skeletal muscle showed the highest Q9/Q10 ratios with significant interindividual variability. Heart, kidney, and particularly brain exhibited lower Q9/Q10 ratios and interindividual variability. In skeletal muscle and heart, the most abundant mCOQ transcript was mCOQ7, followed by mCOQ8, mCOQ2, mPDSS2, mPDSS1, and mCOQ3. In nonmuscular tissues (liver, kidney, and brain) the most abundant mCOQ transcript was mCOQ2, followed by mCOQ7, mCOQ8, mPDSS1, mPDSS2, and mCOQ3. Calorie restriction increased both ubiquinone homologues and mPDSS2 mRNA in skeletal muscle, but mCOQ7 was decreased. In contrast, Q9 and most mCOQ transcripts were decreased in heart. Calorie restriction also modified the Q9/Q10 ratio, which was increased in kidney and decreased in heart without alterations in mPDSS1 or mPDSS2 transcripts. We demonstrate for the first time that unique patterns of mCOQ transcripts exist in muscular and nonmuscular tissues and that Q and COQ genes are targets of calorie restriction in a tissue-specific way.",
keywords = "Calorie restriction, COQ transcripts, Free radicals, Ubiquinone, Ubiquinone biosynthesis",
author = "Cristina Parrado-Fern{\'a}ndez and Guillermo L{\'o}pez-Lluch and Elisabet Rodr{\'i}guez-Bies and Sara Santa-Cruz and Pl{\'a}cido Navas and Ramsey, {Jon J} and Villalba, {Jos{\'e} M.}",
year = "2011",
month = "6",
day = "15",
doi = "10.1016/j.freeradbiomed.2011.03.024",
language = "English (US)",
volume = "50",
pages = "1728--1736",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "12",

}

TY - JOUR

T1 - Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues

AU - Parrado-Fernández, Cristina

AU - López-Lluch, Guillermo

AU - Rodríguez-Bies, Elisabet

AU - Santa-Cruz, Sara

AU - Navas, Plácido

AU - Ramsey, Jon J

AU - Villalba, José M.

PY - 2011/6/15

Y1 - 2011/6/15

N2 - We studied ubiquinone (Q), Q homologue ratio, and steady-state levels of mCOQ transcripts in tissues from mice fed ad libitum or under calorie restriction. Maximum ubiquinone levels on a protein basis were found in kidney and heart, followed by liver, brain, and skeletal muscle. Liver and skeletal muscle showed the highest Q9/Q10 ratios with significant interindividual variability. Heart, kidney, and particularly brain exhibited lower Q9/Q10 ratios and interindividual variability. In skeletal muscle and heart, the most abundant mCOQ transcript was mCOQ7, followed by mCOQ8, mCOQ2, mPDSS2, mPDSS1, and mCOQ3. In nonmuscular tissues (liver, kidney, and brain) the most abundant mCOQ transcript was mCOQ2, followed by mCOQ7, mCOQ8, mPDSS1, mPDSS2, and mCOQ3. Calorie restriction increased both ubiquinone homologues and mPDSS2 mRNA in skeletal muscle, but mCOQ7 was decreased. In contrast, Q9 and most mCOQ transcripts were decreased in heart. Calorie restriction also modified the Q9/Q10 ratio, which was increased in kidney and decreased in heart without alterations in mPDSS1 or mPDSS2 transcripts. We demonstrate for the first time that unique patterns of mCOQ transcripts exist in muscular and nonmuscular tissues and that Q and COQ genes are targets of calorie restriction in a tissue-specific way.

AB - We studied ubiquinone (Q), Q homologue ratio, and steady-state levels of mCOQ transcripts in tissues from mice fed ad libitum or under calorie restriction. Maximum ubiquinone levels on a protein basis were found in kidney and heart, followed by liver, brain, and skeletal muscle. Liver and skeletal muscle showed the highest Q9/Q10 ratios with significant interindividual variability. Heart, kidney, and particularly brain exhibited lower Q9/Q10 ratios and interindividual variability. In skeletal muscle and heart, the most abundant mCOQ transcript was mCOQ7, followed by mCOQ8, mCOQ2, mPDSS2, mPDSS1, and mCOQ3. In nonmuscular tissues (liver, kidney, and brain) the most abundant mCOQ transcript was mCOQ2, followed by mCOQ7, mCOQ8, mPDSS1, mPDSS2, and mCOQ3. Calorie restriction increased both ubiquinone homologues and mPDSS2 mRNA in skeletal muscle, but mCOQ7 was decreased. In contrast, Q9 and most mCOQ transcripts were decreased in heart. Calorie restriction also modified the Q9/Q10 ratio, which was increased in kidney and decreased in heart without alterations in mPDSS1 or mPDSS2 transcripts. We demonstrate for the first time that unique patterns of mCOQ transcripts exist in muscular and nonmuscular tissues and that Q and COQ genes are targets of calorie restriction in a tissue-specific way.

KW - Calorie restriction

KW - COQ transcripts

KW - Free radicals

KW - Ubiquinone

KW - Ubiquinone biosynthesis

UR - http://www.scopus.com/inward/record.url?scp=79956078718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956078718&partnerID=8YFLogxK

U2 - 10.1016/j.freeradbiomed.2011.03.024

DO - 10.1016/j.freeradbiomed.2011.03.024

M3 - Article

C2 - 21447381

AN - SCOPUS:79956078718

VL - 50

SP - 1728

EP - 1736

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 12

ER -