Calorie restriction extends yeast life span by lowering the level of NADH

Su Ju Lin, Ethan Ford, Marcia Haigis, Greg Liszt, Leonard Guarente

Research output: Contribution to journalArticle

482 Citations (Scopus)

Abstract

Calorie restriction (CR) extends life span in a wide variety of species. Previously, we showed that calorie restriction increases the replicative life span in yeast by activating Sir2, a highly conserved NAD-dependent deacetylase. Here we test whether CR activates Sir2 by increasing the NAD/NADH ratio or by regulating the level of nicotinamide, a known inhibitor of Sir2. We show that CR decreases NADH levels, and that NADH is a competitive inhibitor of Sir2. A genetic intervention that specifically decreases NADH levels increases life span, validating the model that NADH regulates yeast longevity in response to CR.

Original languageEnglish (US)
Pages (from-to)12-16
Number of pages5
JournalGenes and Development
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2004

Fingerprint

NAD
Yeasts
Genetic Engineering
Niacinamide

Keywords

  • Calorie restriction
  • Longevity regulation
  • Metabolic signaling

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Lin, S. J., Ford, E., Haigis, M., Liszt, G., & Guarente, L. (2004). Calorie restriction extends yeast life span by lowering the level of NADH. Genes and Development, 18(1), 12-16. https://doi.org/10.1101/gad.1164804

Calorie restriction extends yeast life span by lowering the level of NADH. / Lin, Su Ju; Ford, Ethan; Haigis, Marcia; Liszt, Greg; Guarente, Leonard.

In: Genes and Development, Vol. 18, No. 1, 01.01.2004, p. 12-16.

Research output: Contribution to journalArticle

Lin, SJ, Ford, E, Haigis, M, Liszt, G & Guarente, L 2004, 'Calorie restriction extends yeast life span by lowering the level of NADH', Genes and Development, vol. 18, no. 1, pp. 12-16. https://doi.org/10.1101/gad.1164804
Lin, Su Ju ; Ford, Ethan ; Haigis, Marcia ; Liszt, Greg ; Guarente, Leonard. / Calorie restriction extends yeast life span by lowering the level of NADH. In: Genes and Development. 2004 ; Vol. 18, No. 1. pp. 12-16.
@article{ac3f319348cd43129f5605081aa51c7d,
title = "Calorie restriction extends yeast life span by lowering the level of NADH",
abstract = "Calorie restriction (CR) extends life span in a wide variety of species. Previously, we showed that calorie restriction increases the replicative life span in yeast by activating Sir2, a highly conserved NAD-dependent deacetylase. Here we test whether CR activates Sir2 by increasing the NAD/NADH ratio or by regulating the level of nicotinamide, a known inhibitor of Sir2. We show that CR decreases NADH levels, and that NADH is a competitive inhibitor of Sir2. A genetic intervention that specifically decreases NADH levels increases life span, validating the model that NADH regulates yeast longevity in response to CR.",
keywords = "Calorie restriction, Longevity regulation, Metabolic signaling",
author = "Lin, {Su Ju} and Ethan Ford and Marcia Haigis and Greg Liszt and Leonard Guarente",
year = "2004",
month = "1",
day = "1",
doi = "10.1101/gad.1164804",
language = "English (US)",
volume = "18",
pages = "12--16",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "1",

}

TY - JOUR

T1 - Calorie restriction extends yeast life span by lowering the level of NADH

AU - Lin, Su Ju

AU - Ford, Ethan

AU - Haigis, Marcia

AU - Liszt, Greg

AU - Guarente, Leonard

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Calorie restriction (CR) extends life span in a wide variety of species. Previously, we showed that calorie restriction increases the replicative life span in yeast by activating Sir2, a highly conserved NAD-dependent deacetylase. Here we test whether CR activates Sir2 by increasing the NAD/NADH ratio or by regulating the level of nicotinamide, a known inhibitor of Sir2. We show that CR decreases NADH levels, and that NADH is a competitive inhibitor of Sir2. A genetic intervention that specifically decreases NADH levels increases life span, validating the model that NADH regulates yeast longevity in response to CR.

AB - Calorie restriction (CR) extends life span in a wide variety of species. Previously, we showed that calorie restriction increases the replicative life span in yeast by activating Sir2, a highly conserved NAD-dependent deacetylase. Here we test whether CR activates Sir2 by increasing the NAD/NADH ratio or by regulating the level of nicotinamide, a known inhibitor of Sir2. We show that CR decreases NADH levels, and that NADH is a competitive inhibitor of Sir2. A genetic intervention that specifically decreases NADH levels increases life span, validating the model that NADH regulates yeast longevity in response to CR.

KW - Calorie restriction

KW - Longevity regulation

KW - Metabolic signaling

UR - http://www.scopus.com/inward/record.url?scp=0347128279&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347128279&partnerID=8YFLogxK

U2 - 10.1101/gad.1164804

DO - 10.1101/gad.1164804

M3 - Article

C2 - 14724176

AN - SCOPUS:0347128279

VL - 18

SP - 12

EP - 16

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 1

ER -