Abstract
Purpose: The ketogenic diet (KD) is an effective treatment for intractable epilepsy. However, little is known about its underlying mechanisms. Methods: In this study, in vivo extracellular field responses to angular bundle stimulation were recorded in the dentate gyrus of Sprague-Dawley rats fed one of three diets: ketogenic calorie-restricted (KCR), normal calorie-restricted (NCR), or normal ad libitum (NAL). Input/output curves and paired-pulse relations were used to assess network excitability. A maximal dentate activation (MDA) protocol was used to measure electrographic seizure threshold and duration. Results: Animals fed calorie-restricted (CR) diets exhibited greater paired-pulse inhibition, an elevated MDA threshold, and an absence of spreading depression-like events compared with ad libitum-fed controls, In the MDA model of epileptogenesis, the rate of increase in electrographic seizure duration after repeated stimuli was markedly reduced in KCR-fed animals compared with NCR- and NAL-fed controls. Conclusions: These data suggest that CR, by itself, can be anticonvulsant, and treatment with a KCR diet may be both anticonvulsant and antiepileptogenic.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 752-760 |
| Number of pages | 9 |
| Journal | Epilepsia |
| Volume | 44 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2003 |
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Keywords
- βHydroxybutyrate
- Calorie restriction
- Dentate gyrus
- Epilepsy
- Ketogenic diet
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
Cite this
Calorie restriction and ketogenic diet diminish neuronal excitability in rat dentate gyrus in vivo. / Bough, Kristopher J.; Schwartzkroin, Philip A; Rho, Jong M.
In: Epilepsia, Vol. 44, No. 6, 01.06.2003, p. 752-760.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calorie restriction and ketogenic diet diminish neuronal excitability in rat dentate gyrus in vivo
AU - Bough, Kristopher J.
AU - Schwartzkroin, Philip A
AU - Rho, Jong M.
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Purpose: The ketogenic diet (KD) is an effective treatment for intractable epilepsy. However, little is known about its underlying mechanisms. Methods: In this study, in vivo extracellular field responses to angular bundle stimulation were recorded in the dentate gyrus of Sprague-Dawley rats fed one of three diets: ketogenic calorie-restricted (KCR), normal calorie-restricted (NCR), or normal ad libitum (NAL). Input/output curves and paired-pulse relations were used to assess network excitability. A maximal dentate activation (MDA) protocol was used to measure electrographic seizure threshold and duration. Results: Animals fed calorie-restricted (CR) diets exhibited greater paired-pulse inhibition, an elevated MDA threshold, and an absence of spreading depression-like events compared with ad libitum-fed controls, In the MDA model of epileptogenesis, the rate of increase in electrographic seizure duration after repeated stimuli was markedly reduced in KCR-fed animals compared with NCR- and NAL-fed controls. Conclusions: These data suggest that CR, by itself, can be anticonvulsant, and treatment with a KCR diet may be both anticonvulsant and antiepileptogenic.
AB - Purpose: The ketogenic diet (KD) is an effective treatment for intractable epilepsy. However, little is known about its underlying mechanisms. Methods: In this study, in vivo extracellular field responses to angular bundle stimulation were recorded in the dentate gyrus of Sprague-Dawley rats fed one of three diets: ketogenic calorie-restricted (KCR), normal calorie-restricted (NCR), or normal ad libitum (NAL). Input/output curves and paired-pulse relations were used to assess network excitability. A maximal dentate activation (MDA) protocol was used to measure electrographic seizure threshold and duration. Results: Animals fed calorie-restricted (CR) diets exhibited greater paired-pulse inhibition, an elevated MDA threshold, and an absence of spreading depression-like events compared with ad libitum-fed controls, In the MDA model of epileptogenesis, the rate of increase in electrographic seizure duration after repeated stimuli was markedly reduced in KCR-fed animals compared with NCR- and NAL-fed controls. Conclusions: These data suggest that CR, by itself, can be anticonvulsant, and treatment with a KCR diet may be both anticonvulsant and antiepileptogenic.
KW - βHydroxybutyrate
KW - Calorie restriction
KW - Dentate gyrus
KW - Epilepsy
KW - Ketogenic diet
UR - http://www.scopus.com/inward/record.url?scp=0038305724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038305724&partnerID=8YFLogxK
U2 - 10.1046/j.1528-1157.2003.55502.x
DO - 10.1046/j.1528-1157.2003.55502.x
M3 - Article
C2 - 12790887
AN - SCOPUS:0038305724
VL - 44
SP - 752
EP - 760
JO - Epilepsia
JF - Epilepsia
SN - 0013-9580
IS - 6
ER -