Caloric restriction influences hydrogen peroxide generation in mitochondrial sub-populations from mouse liver

Kevork Hagopian, Yana Chen, Keira Simmons Domer, Robert Soo Hoo, Trevor Bentley, Roger B. McDonald, Jon J Ramsey

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Calorie restriction (CR) has been shown to decrease H2O 2 production in liver mitochondria, although it is not known if this is due to uniform changes in all mitochondria or changes in particular mitochondrial sub-populations. To address this issue, liver mitochondria from control and CR mice were fractionated using differential centrifugation at 1,000 g, 3,000 g and 10,000 g into distinct populations labeled as M1, M3 and M10, respectively. Mitochondrial protein levels, respiration and H2O 2 production were measured in each fraction. CR resulted in a decrease in total protein (mg) in each fraction, although this difference disappeared when adjusted for liver weight (mg protein/g liver weight). No differences in respiration (State 3 or 4) were observed between control and CR mice in any of the mitochondrial fractions. CR decreased H2O 2 production in all fractions when mitochondria respired on succinate (Succ), succ+antimycin A (Succ+AA) or pyruvate/malate+rotenone (P/M+ROT). Thus, CR decreased reactive oxygen species (ROS) production under conditions which stimulate mitochondrial complex I ROS production under both forward (P/M+ROT) and backward (Succ & Succ+AA) electron flow. The results indicate that CR decreases H2O2 production in all liver mitochondrial fractions due to a decrease in capacity for ROS production by complex I of the electron transport chain.

Original languageEnglish (US)
Pages (from-to)227-236
Number of pages10
JournalJournal of Bioenergetics and Biomembranes
Issue number3
StatePublished - 2011


  • Caloric restriction
  • Hydrogen peroxide
  • Mitochondria
  • Mouse liver
  • Reactive oxygen species
  • Respiration

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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