Abstract
Calorie restriction (CR) has been shown to decrease H2O 2 production in liver mitochondria, although it is not known if this is due to uniform changes in all mitochondria or changes in particular mitochondrial sub-populations. To address this issue, liver mitochondria from control and CR mice were fractionated using differential centrifugation at 1,000 g, 3,000 g and 10,000 g into distinct populations labeled as M1, M3 and M10, respectively. Mitochondrial protein levels, respiration and H2O 2 production were measured in each fraction. CR resulted in a decrease in total protein (mg) in each fraction, although this difference disappeared when adjusted for liver weight (mg protein/g liver weight). No differences in respiration (State 3 or 4) were observed between control and CR mice in any of the mitochondrial fractions. CR decreased H2O 2 production in all fractions when mitochondria respired on succinate (Succ), succ+antimycin A (Succ+AA) or pyruvate/malate+rotenone (P/M+ROT). Thus, CR decreased reactive oxygen species (ROS) production under conditions which stimulate mitochondrial complex I ROS production under both forward (P/M+ROT) and backward (Succ & Succ+AA) electron flow. The results indicate that CR decreases H2O2 production in all liver mitochondrial fractions due to a decrease in capacity for ROS production by complex I of the electron transport chain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 227-236 |
| Number of pages | 10 |
| Journal | Journal of Bioenergetics and Biomembranes |
| Volume | 43 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2011 |
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Keywords
- Caloric restriction
- Hydrogen peroxide
- Mitochondria
- Mouse liver
- Reactive oxygen species
- Respiration
ASJC Scopus subject areas
- Physiology
- Cell Biology
Cite this
Caloric restriction influences hydrogen peroxide generation in mitochondrial sub-populations from mouse liver. / Hagopian, Kevork; Chen, Yana; Simmons Domer, Keira; Soo Hoo, Robert; Bentley, Trevor; McDonald, Roger B.; Ramsey, Jon J.
In: Journal of Bioenergetics and Biomembranes, Vol. 43, No. 3, 2011, p. 227-236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Caloric restriction influences hydrogen peroxide generation in mitochondrial sub-populations from mouse liver
AU - Hagopian, Kevork
AU - Chen, Yana
AU - Simmons Domer, Keira
AU - Soo Hoo, Robert
AU - Bentley, Trevor
AU - McDonald, Roger B.
AU - Ramsey, Jon J
PY - 2011
Y1 - 2011
N2 - Calorie restriction (CR) has been shown to decrease H2O 2 production in liver mitochondria, although it is not known if this is due to uniform changes in all mitochondria or changes in particular mitochondrial sub-populations. To address this issue, liver mitochondria from control and CR mice were fractionated using differential centrifugation at 1,000 g, 3,000 g and 10,000 g into distinct populations labeled as M1, M3 and M10, respectively. Mitochondrial protein levels, respiration and H2O 2 production were measured in each fraction. CR resulted in a decrease in total protein (mg) in each fraction, although this difference disappeared when adjusted for liver weight (mg protein/g liver weight). No differences in respiration (State 3 or 4) were observed between control and CR mice in any of the mitochondrial fractions. CR decreased H2O 2 production in all fractions when mitochondria respired on succinate (Succ), succ+antimycin A (Succ+AA) or pyruvate/malate+rotenone (P/M+ROT). Thus, CR decreased reactive oxygen species (ROS) production under conditions which stimulate mitochondrial complex I ROS production under both forward (P/M+ROT) and backward (Succ & Succ+AA) electron flow. The results indicate that CR decreases H2O2 production in all liver mitochondrial fractions due to a decrease in capacity for ROS production by complex I of the electron transport chain.
AB - Calorie restriction (CR) has been shown to decrease H2O 2 production in liver mitochondria, although it is not known if this is due to uniform changes in all mitochondria or changes in particular mitochondrial sub-populations. To address this issue, liver mitochondria from control and CR mice were fractionated using differential centrifugation at 1,000 g, 3,000 g and 10,000 g into distinct populations labeled as M1, M3 and M10, respectively. Mitochondrial protein levels, respiration and H2O 2 production were measured in each fraction. CR resulted in a decrease in total protein (mg) in each fraction, although this difference disappeared when adjusted for liver weight (mg protein/g liver weight). No differences in respiration (State 3 or 4) were observed between control and CR mice in any of the mitochondrial fractions. CR decreased H2O 2 production in all fractions when mitochondria respired on succinate (Succ), succ+antimycin A (Succ+AA) or pyruvate/malate+rotenone (P/M+ROT). Thus, CR decreased reactive oxygen species (ROS) production under conditions which stimulate mitochondrial complex I ROS production under both forward (P/M+ROT) and backward (Succ & Succ+AA) electron flow. The results indicate that CR decreases H2O2 production in all liver mitochondrial fractions due to a decrease in capacity for ROS production by complex I of the electron transport chain.
KW - Caloric restriction
KW - Hydrogen peroxide
KW - Mitochondria
KW - Mouse liver
KW - Reactive oxygen species
KW - Respiration
UR - http://www.scopus.com/inward/record.url?scp=85027926293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027926293&partnerID=8YFLogxK
U2 - 10.1007/s10863-011-9353-8
DO - 10.1007/s10863-011-9353-8
M3 - Article
AN - SCOPUS:85027926293
VL - 43
SP - 227
EP - 236
JO - Journal of Bioenergetics and Biomembranes
JF - Journal of Bioenergetics and Biomembranes
SN - 0145-479X
IS - 3
ER -