Caloric restriction increases gluconeogenic and transaminase enzyme activities in mouse liver

Kevork Hagopian, Jon J Ramsey, Richard Weindruch

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Long-term caloric restriction (CR) has been shown to extend maximum life span in laboratory rodents. We investigated the activities of gluconeogenic and transaminase enzymes in the livers of old and young mice fed either control or calorie-restricted diets. Livers were sampled 48h after the last scheduled feeding time. Old mice on CR showed significant increases in the activities of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase and glucose-6-phosphatase when compared with controls, indicating increased gluconeogenesis. Increased activities of tyrosine, tryptophan, histidine, phenylalanine, alanine and aspartate transaminases, as well as of malate and glutamate dehydrogenases were also observed, while branched-chain amino acid transaminase was unchanged. Young mice on CR showed a significant increase only in the phosphoenolpyruvate carboxykinase activity in the gluconeogenic pathway, while transaminases were increased significantly, except for tryptophan and branched-chain amino acid transaminases. Glutamate dehydrogenase also showed increased activity but malate dehydrogenase was unchanged. Increases in the level of acetyl-CoA and [Acetyl-CoA]/[CoA] ratio were observed only in the old CR mice. Our results demonstrate increased gluconeogenic activity in CR mice and are consistent with a state of increased hepatic gluconeogenesis and protein turnover during CR.

Original languageEnglish (US)
Pages (from-to)267-278
Number of pages12
JournalExperimental Gerontology
Volume38
Issue number3
DOIs
StatePublished - Mar 1 2003

Fingerprint

Caloric Restriction
Enzyme activity
Transaminases
Liver
Enzymes
Glutamate Dehydrogenase
Malate Dehydrogenase
Acetyl Coenzyme A
Phosphoenolpyruvate
Gluconeogenesis
Tryptophan
Tryptophan Transaminase
Pyruvate Carboxylase
Fructose-Bisphosphatase
Glucose-6-Phosphatase
Coenzyme A
Nutrition
Aspartate Aminotransferases
Alanine Transaminase
Histidine

Keywords

  • Caloric restriction
  • Fructose-1,6-bisphosphatase
  • Gluconeogenesis
  • Glucose-6-phosphatase
  • Phosphoenolpyruvate carboxykinase
  • Pyruvate carboxylase
  • Transaminase

ASJC Scopus subject areas

  • Aging
  • Medicine(all)

Cite this

Caloric restriction increases gluconeogenic and transaminase enzyme activities in mouse liver. / Hagopian, Kevork; Ramsey, Jon J; Weindruch, Richard.

In: Experimental Gerontology, Vol. 38, No. 3, 01.03.2003, p. 267-278.

Research output: Contribution to journalArticle

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