California mussels (Mytilus californianus) as sentinels for marine contamination with Sarcocystis neurona

Lauren Michaels, Daniel Rejmanek, Beatriz Aguilar, Patricia A Conrad, Karen Shapiro

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sarcocystis neurona is a terrestrial parasite that can cause fatal encephalitis in the endangered Southern sea otter (Enhydra lutris nereis). To date, neither risk factors associated with marine contamination nor the route of S. neurona infection to marine mammals has been described. This study evaluated coastal S. neurona contamination using California mussels (Mytilus californianus) as sentinels for pathogen pollution. A field investigation was designed to test the hypotheses that (1) mussels can serve as sentinels for S. neurona contamination, and (2) S. neurona contamination in mussels would be highest during the rainy season and in mussels collected near freshwater. Initial validation of molecular assays through sporocyst spiking experiments revealed the ITS-1500 assay to be most sensitive for detection of S. neurona, consistently yielding parasite amplification at concentrations 5 sporocysts/1 mL mussel haemolymph. Assays were then applied on 959 wild-caught mussels, with detection of S. neurona confirmed using sequence analysis in three mussels. Validated molecular assays for S. neurona detection in mussels provide a novel toolset for investigating marine contamination with this parasite, while confirmation of S. neurona in wild mussels suggests that uptake by invertebrates may serve as a route of transmission to susceptible marine animals.

Original languageEnglish (US)
Pages (from-to)762-769
Number of pages8
JournalParasitology
Volume143
Issue number6
DOIs
StatePublished - May 1 2016

Fingerprint

Sarcocystis
Mytilus
Sarcocystis neurona
Bivalvia
mussels
Otters
Parasites
Oocysts
assays
parasites
cyhalothrin
Mytilus californianus
Hemolymph
marine mammals
Encephalitis
Invertebrates
encephalitis
Fresh Water
hemolymph
Sequence Analysis

Keywords

  • California mussels (Mytilus californianus)
  • Detection
  • Haemolymph
  • Marine
  • Sarcocystis neurona
  • Sea otters (Enhydra lutris nereis)
  • Sporocysts

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

California mussels (Mytilus californianus) as sentinels for marine contamination with Sarcocystis neurona. / Michaels, Lauren; Rejmanek, Daniel; Aguilar, Beatriz; Conrad, Patricia A; Shapiro, Karen.

In: Parasitology, Vol. 143, No. 6, 01.05.2016, p. 762-769.

Research output: Contribution to journalArticle

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