Calculation of the gating charge for the Kv1.2 voltage-activated potassium channel

Fatemeh Khalili-Araghi, Vishwanath Jogini, Vladimir Yarov-Yarovoy, Emad Tajkhorshid, Benoît Roux, Klaus Schulten

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The atomic models of the Kv1.2 potassium channel in the active and resting state, originally presented elsewhere, are here refined using molecular dynamics simulations in an explicit membrane-solvent environment. With a minor adjustment of the orientation of the first arginine along the S4 segment, the total gating charge of the channel determined from >0.5 μs of molecular dynamics simulation is ∼12-12.7 e, in good accord with experimental estimates for the Shaker potassium channel, indicating that the final models offer a realistic depiction of voltage-gating. In the resting state of Kv1.2, the S4 segment in the voltage-sensing domain (VSD) spontaneously converts into a 310 helix over a stretch of 10 residues. The 310 helical conformation orients the gating arginines on S4 toward a water-filled crevice within the VSD and allows salt-bridge interactions with negatively charged residues along S2 and S3. Free energy calculations of the fractional transmembrane potential, acting upon key charged residues of the VSD, reveals that the applied field varies rapidly over a narrow region of 10-15 Å corresponding to the outer leaflet of the bilayer. The focused field allows the transfer of a large gating charge without translocation of S4 across the membrane.

Original languageEnglish (US)
Pages (from-to)2189-2198
Number of pages10
JournalBiophysical Journal
Volume98
Issue number10
DOIs
StatePublished - May 19 2010
Externally publishedYes

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Potassium Channels
Molecular Dynamics Simulation
Arginine
Kv1.2 Potassium Channel
Shaker Superfamily of Potassium Channels
Membranes
Membrane Potentials
Salts
Water

ASJC Scopus subject areas

  • Biophysics

Cite this

Calculation of the gating charge for the Kv1.2 voltage-activated potassium channel. / Khalili-Araghi, Fatemeh; Jogini, Vishwanath; Yarov-Yarovoy, Vladimir; Tajkhorshid, Emad; Roux, Benoît; Schulten, Klaus.

In: Biophysical Journal, Vol. 98, No. 10, 19.05.2010, p. 2189-2198.

Research output: Contribution to journalArticle

Khalili-Araghi, F, Jogini, V, Yarov-Yarovoy, V, Tajkhorshid, E, Roux, B & Schulten, K 2010, 'Calculation of the gating charge for the Kv1.2 voltage-activated potassium channel', Biophysical Journal, vol. 98, no. 10, pp. 2189-2198. https://doi.org/10.1016/j.bpj.2010.02.056
Khalili-Araghi, Fatemeh ; Jogini, Vishwanath ; Yarov-Yarovoy, Vladimir ; Tajkhorshid, Emad ; Roux, Benoît ; Schulten, Klaus. / Calculation of the gating charge for the Kv1.2 voltage-activated potassium channel. In: Biophysical Journal. 2010 ; Vol. 98, No. 10. pp. 2189-2198.
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