Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC

Kanta Horie, Hiroshi Kimura, Tohru Ikegami, Akira Iwatsuka, Nabil Saad, Oliver Fiehn, Nobuo Tanaka

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Theoretical calculations are presented to optimize modulation period for maximum total peak capacity in comprehensive two-dimensional HPLC (2D-HPLC) taking into account the effect of modulation on the apparent peak capacity of the first-dimension (1D) separation. Results indicate that modulation periods are most favorable when they are adjusted to ∼2.2-4 times the standard deviation of a 1D peak in order to avoid excessively short run times at the second dimension (2D). Data are presented that effective peak capacities of several thousand in 60 min can be expected for practical 2D-HPLC conditions, utilizing 1D gradient elution followed by 2D isocratic elution, that remain at ∼50-70% of the theoretical maximum peak capacity. This work suggests that lower modulation frequencies and longer 2D separation times than previously proposed are favorable under realistic chromatographic conditions, alleviating some practical problems associated with 2D-HPLC.

Original languageEnglish (US)
Pages (from-to)3764-3770
Number of pages7
JournalAnalytical Chemistry
Volume79
Issue number10
DOIs
StatePublished - May 15 2007

Fingerprint

Modulation
Frequency modulation

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Horie, K., Kimura, H., Ikegami, T., Iwatsuka, A., Saad, N., Fiehn, O., & Tanaka, N. (2007). Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC. Analytical Chemistry, 79(10), 3764-3770. https://doi.org/10.1021/ac062002t

Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC. / Horie, Kanta; Kimura, Hiroshi; Ikegami, Tohru; Iwatsuka, Akira; Saad, Nabil; Fiehn, Oliver; Tanaka, Nobuo.

In: Analytical Chemistry, Vol. 79, No. 10, 15.05.2007, p. 3764-3770.

Research output: Contribution to journalArticle

Horie, K, Kimura, H, Ikegami, T, Iwatsuka, A, Saad, N, Fiehn, O & Tanaka, N 2007, 'Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC', Analytical Chemistry, vol. 79, no. 10, pp. 3764-3770. https://doi.org/10.1021/ac062002t
Horie K, Kimura H, Ikegami T, Iwatsuka A, Saad N, Fiehn O et al. Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC. Analytical Chemistry. 2007 May 15;79(10):3764-3770. https://doi.org/10.1021/ac062002t
Horie, Kanta ; Kimura, Hiroshi ; Ikegami, Tohru ; Iwatsuka, Akira ; Saad, Nabil ; Fiehn, Oliver ; Tanaka, Nobuo. / Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC. In: Analytical Chemistry. 2007 ; Vol. 79, No. 10. pp. 3764-3770.
@article{623d0b74a0e0446d8ce03b3b190bbe5f,
title = "Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC",
abstract = "Theoretical calculations are presented to optimize modulation period for maximum total peak capacity in comprehensive two-dimensional HPLC (2D-HPLC) taking into account the effect of modulation on the apparent peak capacity of the first-dimension (1D) separation. Results indicate that modulation periods are most favorable when they are adjusted to ∼2.2-4 times the standard deviation of a 1D peak in order to avoid excessively short run times at the second dimension (2D). Data are presented that effective peak capacities of several thousand in 60 min can be expected for practical 2D-HPLC conditions, utilizing 1D gradient elution followed by 2D isocratic elution, that remain at ∼50-70{\%} of the theoretical maximum peak capacity. This work suggests that lower modulation frequencies and longer 2D separation times than previously proposed are favorable under realistic chromatographic conditions, alleviating some practical problems associated with 2D-HPLC.",
author = "Kanta Horie and Hiroshi Kimura and Tohru Ikegami and Akira Iwatsuka and Nabil Saad and Oliver Fiehn and Nobuo Tanaka",
year = "2007",
month = "5",
day = "15",
doi = "10.1021/ac062002t",
language = "English (US)",
volume = "79",
pages = "3764--3770",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC

AU - Horie, Kanta

AU - Kimura, Hiroshi

AU - Ikegami, Tohru

AU - Iwatsuka, Akira

AU - Saad, Nabil

AU - Fiehn, Oliver

AU - Tanaka, Nobuo

PY - 2007/5/15

Y1 - 2007/5/15

N2 - Theoretical calculations are presented to optimize modulation period for maximum total peak capacity in comprehensive two-dimensional HPLC (2D-HPLC) taking into account the effect of modulation on the apparent peak capacity of the first-dimension (1D) separation. Results indicate that modulation periods are most favorable when they are adjusted to ∼2.2-4 times the standard deviation of a 1D peak in order to avoid excessively short run times at the second dimension (2D). Data are presented that effective peak capacities of several thousand in 60 min can be expected for practical 2D-HPLC conditions, utilizing 1D gradient elution followed by 2D isocratic elution, that remain at ∼50-70% of the theoretical maximum peak capacity. This work suggests that lower modulation frequencies and longer 2D separation times than previously proposed are favorable under realistic chromatographic conditions, alleviating some practical problems associated with 2D-HPLC.

AB - Theoretical calculations are presented to optimize modulation period for maximum total peak capacity in comprehensive two-dimensional HPLC (2D-HPLC) taking into account the effect of modulation on the apparent peak capacity of the first-dimension (1D) separation. Results indicate that modulation periods are most favorable when they are adjusted to ∼2.2-4 times the standard deviation of a 1D peak in order to avoid excessively short run times at the second dimension (2D). Data are presented that effective peak capacities of several thousand in 60 min can be expected for practical 2D-HPLC conditions, utilizing 1D gradient elution followed by 2D isocratic elution, that remain at ∼50-70% of the theoretical maximum peak capacity. This work suggests that lower modulation frequencies and longer 2D separation times than previously proposed are favorable under realistic chromatographic conditions, alleviating some practical problems associated with 2D-HPLC.

UR - http://www.scopus.com/inward/record.url?scp=34249038946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249038946&partnerID=8YFLogxK

U2 - 10.1021/ac062002t

DO - 10.1021/ac062002t

M3 - Article

C2 - 17437330

AN - SCOPUS:34249038946

VL - 79

SP - 3764

EP - 3770

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 10

ER -