Calcium Transients in 1B5 Myotubes Lacking Ryanodine Receptors Are Related to Inositol Trisphosphate Receptors

Manuel Estrada, Cesar Cárdenas, José L. Liberona, M. Angélica Carrasco, Gregory A. Mignery, Paul D. Allen, Enrique Jaimovich

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Potassium depolarization of skeletal myotubes evokes slow calcium waves that are unrelated to contraction and involve the cell nucleus (Jaimovich, E., Reyes, R., Liberona, J. L., and Powell, J. A. (2000) Am. J. Physiol. 278, C998-C1010). Studies were done in both the 1B5 (Ry53-/-) murine "dyspedic" myoblast cell line, which does not express any ryanodine receptor isoforms (Moore, R. A., Nguyen, H., Galceran, J., Pessah, I. N., and Allen, P. D. (1998) J. Cell Biol. 140, 843-851), and C2C 12 cells, a myoblast cell line that expresses all three isoforms. Although 1B5 cells lack ryanodine binding, they bind tritiated inositol (1,4,5)-trisphosphate. Both type 1 and type 3 inositol trisphosphate receptors were immuno-located in the nuclei of both cell types and were visualized by Western blot analysis. After stimulation with 47 mM K+, inositol trisphosphate mass raised transiently in both cell types. Both fast calcium increase and slow propagated calcium signals were seen in C2C 12 myotubes. However, 1B5 myotubes (as well as ryanodine-treated C2C12 myotubes) displayed only a long-lasting, non-propagating calcium increase, particularly evident in the nuclei. Calcium signals in 1B5 myotubes were almost completely blocked by inhibitors of the inositol trisphosphate pathway: U73122, 2-aminoethoxydiphenyl borate, or xestospongin C. Results support the hypothesis that inositol trisphosphate mediates slow calcium signals in muscle cell ryanodine receptors, having a role in their time course and propagation.

Original languageEnglish (US)
Pages (from-to)22868-22874
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number25
DOIs
StatePublished - Jun 22 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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    Estrada, M., Cárdenas, C., Liberona, J. L., Carrasco, M. A., Mignery, G. A., Allen, P. D., & Jaimovich, E. (2001). Calcium Transients in 1B5 Myotubes Lacking Ryanodine Receptors Are Related to Inositol Trisphosphate Receptors. Journal of Biological Chemistry, 276(25), 22868-22874. https://doi.org/10.1074/jbc.M100118200