Calcium retrieval from vacuolar pools: Characterization of a vacuolar calcium channel

Angela C Gelli, Eduardo Blumwald

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Voltage patch-clamp experiments at the whole-vacuole and single-channel levels were employed to study the retrieval of Ca2+ from vacuoles into the cytoplasm in sugar beet cell (Beta vulgaris L.) suspension cultures. Channels allowing the movement of Ca2+ out of the vacuole were identified at physiological conditions of pH, vacuolar membrane potential, and vacuole/cytoplasm Ca2+ concentrations. The operation of the channel was voltage dependent and inositol-1,4,5-triphosphate insensitive and displayed high selectivity for Ca2+ ions. These channels bear similarities to the dihydropyridine-sensitive L-type Ca2+ channels from animal cells. Bay K-8644, an agonist, increased the frequency of channel openings, whereas nifedipine, an antagonist, reduced the channel activity. Both effects were elicited only from the vacuolar side of the channel. Channel activities were also inhibited by verapamil, La3+, and cytoplasmic Ca2+ concentrations higher than 1 × 10-6 M. The modulation of the channel currents by cytoplasmic Ca2+ would suggest the role of these channels in triggering the initiation of signal transduction processes in plant cells.

Original languageEnglish (US)
Pages (from-to)1139-1146
Number of pages8
JournalPlant Physiology
Volume102
Issue number4
StatePublished - Aug 1993
Externally publishedYes

Fingerprint

calcium channels
Calcium Channels
Vacuoles
Calcium
calcium
Beta vulgaris
vacuoles
Cytoplasm
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
Inositol 1,4,5-Trisphosphate
Plant Cells
Nifedipine
Verapamil
Membrane Potentials
cytoplasm
Signal Transduction
Suspensions
Ions
verapamil
cells

ASJC Scopus subject areas

  • Plant Science

Cite this

Calcium retrieval from vacuolar pools : Characterization of a vacuolar calcium channel. / Gelli, Angela C; Blumwald, Eduardo.

In: Plant Physiology, Vol. 102, No. 4, 08.1993, p. 1139-1146.

Research output: Contribution to journalArticle

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