Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks

Ang Li; Jung Hwa Cho; Brian Reid; Chun Chih Tseng; Lian He; Peng Tan; Chao Yuan Yeh; Ping Wu; Yuwei Li; Randall B. Widelitz; Yubin Zhou; Min Zhao; Robert H. Chow; Cheng Ming Chuong

doi:10.1038/s41467-018-07661-5

Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks

Ang Li, Jung Hwa Cho, Brian Reid, Chun Chih Tseng, Lian He, Peng Tan, Chao Yuan Yeh, Ping Wu, Yuwei Li, Randall B. Widelitz, Yubin Zhou, Min Zhao, Robert H. Chow, Cheng Ming Chuong

Dermatology

Research output: Contribution to journal › Article

6 Scopus citations

Abstract

Collective cell migration mediates multiple tissue morphogenesis processes. Yet how multi-dimensional mesenchymal cell movements are coordinated remains mostly unknown. Here we report that coordinated mesenchymal cell migration during chicken feather elongation is accompanied by dynamic changes of bioelectric currents. Transcriptome profiling and functional assays implicate contributions from functional voltage-gated Ca ²⁺ channels (VGCCs), Connexin-43 based gap junctions, and Ca ²⁺ release activated Ca ²⁺ (CRAC) channels. 4-Dimensional Ca ²⁺ imaging reveals that the Sonic hedgehog-responsive mesenchymal cells display synchronized Ca ²⁺ oscillations, which expand progressively in area during feather elongation. Inhibiting VGCCs, gap junctions, or Sonic hedgehog signaling alters the mesenchymal Ca ²⁺ landscape, cell movement patterns and feather bud elongation. Ca ²⁺ oscillations induced by cyclic activation of opto-cCRAC channels enhance feather bud elongation. Functional disruption experiments and promoter analysis implicate synergistic Hedgehog and WNT/β-Catenin signaling in activating Connexin-43 expression, establishing gap junction networks synchronizing the Ca ²⁺ profile among cells, thereby coordinating cell movement patterns.

Original language	English (US)
Article number	5377
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-07661-5
State	Published - Dec 1 2018

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Li, A., Cho, J. H., Reid, B., Tseng, C. C., He, L., Tan, P., Yeh, C. Y., Wu, P., Li, Y., Widelitz, R. B., Zhou, Y., Zhao, M., Chow, R. H., & Chuong, C. M. (2018). Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks. Nature communications, 9(1), [5377]. https://doi.org/10.1038/s41467-018-07661-5

Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks. / Li, Ang; Cho, Jung Hwa; Reid, Brian; Tseng, Chun Chih; He, Lian; Tan, Peng; Yeh, Chao Yuan; Wu, Ping; Li, Yuwei; Widelitz, Randall B.; Zhou, Yubin; Zhao, Min; Chow, Robert H.; Chuong, Cheng Ming.

In: Nature communications, Vol. 9, No. 1, 5377, 01.12.2018.

Research output: Contribution to journal › Article

Li, Ang ; Cho, Jung Hwa ; Reid, Brian ; Tseng, Chun Chih ; He, Lian ; Tan, Peng ; Yeh, Chao Yuan ; Wu, Ping ; Li, Yuwei ; Widelitz, Randall B. ; Zhou, Yubin ; Zhao, Min ; Chow, Robert H. ; Chuong, Cheng Ming. / Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks. In: Nature communications. 2018 ; Vol. 9, No. 1.

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abstract = " Collective cell migration mediates multiple tissue morphogenesis processes. Yet how multi-dimensional mesenchymal cell movements are coordinated remains mostly unknown. Here we report that coordinated mesenchymal cell migration during chicken feather elongation is accompanied by dynamic changes of bioelectric currents. Transcriptome profiling and functional assays implicate contributions from functional voltage-gated Ca 2+ channels (VGCCs), Connexin-43 based gap junctions, and Ca 2+ release activated Ca 2+ (CRAC) channels. 4-Dimensional Ca 2+ imaging reveals that the Sonic hedgehog-responsive mesenchymal cells display synchronized Ca 2+ oscillations, which expand progressively in area during feather elongation. Inhibiting VGCCs, gap junctions, or Sonic hedgehog signaling alters the mesenchymal Ca 2+ landscape, cell movement patterns and feather bud elongation. Ca 2+ oscillations induced by cyclic activation of opto-cCRAC channels enhance feather bud elongation. Functional disruption experiments and promoter analysis implicate synergistic Hedgehog and WNT/β-Catenin signaling in activating Connexin-43 expression, establishing gap junction networks synchronizing the Ca 2+ profile among cells, thereby coordinating cell movement patterns. ",

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