Calcium currents of differentiated mouse neuroblastoma cells

N. S. Veselovskii; N. Kh Pogorelaya; Alla F Fomina

doi:10.1007/BF01053499

Calcium currents of differentiated mouse neuroblastoma cells

N. S. Veselovskii, N. Kh Pogorelaya, Alla F Fomina

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Abstract

Differentiated mouse neuroblastoma cells (line C 1300, clone N-18-TG₂A₁) were investigated by intracellular dialysis. A slow component was found in the potential-dependent inward current of these cells. The results of investigation of changes in amplitude of this component during variation of the ionic composition of the external and internal solutions showed that this component is due to transport of calcium ions. A calcium current was observed in all cells tested. The region of its activation was between -70 and -65 mV; maximal values of this current were reached when the membrane potential was between -30 and -40 mV. The kinetic characteristics of this current were examined. In its kinetics and potential-dependence, this calcium current of the mouse neuroblastoma cell membrane is analogous to the fast component of the calcium current in normal neurons of rat spinal ganglia.

Original language	English (US)
Pages (from-to)	419-422
Number of pages	4
Journal	Neurophysiology
Volume	16
Issue number	4
DOIs	https://doi.org/10.1007/BF01053499
State	Published - Jul 1984
Externally published	Yes

ASJC Scopus subject areas

Neuroscience(all)
Physiology

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abstract = "Differentiated mouse neuroblastoma cells (line C 1300, clone N-18-TG2A1) were investigated by intracellular dialysis. A slow component was found in the potential-dependent inward current of these cells. The results of investigation of changes in amplitude of this component during variation of the ionic composition of the external and internal solutions showed that this component is due to transport of calcium ions. A calcium current was observed in all cells tested. The region of its activation was between -70 and -65 mV; maximal values of this current were reached when the membrane potential was between -30 and -40 mV. The kinetic characteristics of this current were examined. In its kinetics and potential-dependence, this calcium current of the mouse neuroblastoma cell membrane is analogous to the fast component of the calcium current in normal neurons of rat spinal ganglia.",

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AU - Veselovskii, N. S.

AU - Pogorelaya, N. Kh

AU - Fomina, Alla F

PY - 1984/7

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N2 - Differentiated mouse neuroblastoma cells (line C 1300, clone N-18-TG2A1) were investigated by intracellular dialysis. A slow component was found in the potential-dependent inward current of these cells. The results of investigation of changes in amplitude of this component during variation of the ionic composition of the external and internal solutions showed that this component is due to transport of calcium ions. A calcium current was observed in all cells tested. The region of its activation was between -70 and -65 mV; maximal values of this current were reached when the membrane potential was between -30 and -40 mV. The kinetic characteristics of this current were examined. In its kinetics and potential-dependence, this calcium current of the mouse neuroblastoma cell membrane is analogous to the fast component of the calcium current in normal neurons of rat spinal ganglia.

AB - Differentiated mouse neuroblastoma cells (line C 1300, clone N-18-TG2A1) were investigated by intracellular dialysis. A slow component was found in the potential-dependent inward current of these cells. The results of investigation of changes in amplitude of this component during variation of the ionic composition of the external and internal solutions showed that this component is due to transport of calcium ions. A calcium current was observed in all cells tested. The region of its activation was between -70 and -65 mV; maximal values of this current were reached when the membrane potential was between -30 and -40 mV. The kinetic characteristics of this current were examined. In its kinetics and potential-dependence, this calcium current of the mouse neuroblastoma cell membrane is analogous to the fast component of the calcium current in normal neurons of rat spinal ganglia.

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Calcium currents of differentiated mouse neuroblastoma cells

Abstract

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