Calcium channel blockers inhibit galvanotaxis in human keratinocytes

Donna R. Trollinger, Roslyn Rivkah Isseroff, Richard Nuccitelli

Research output: Contribution to journalArticle

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Abstract

Directed migration of keratinocytes is essential for wound healing. The migration of human keratinocytes in vitro is strongly influenced by the presence of a physiological electric field and these cells migrate towards the negative pole of such a field (galvanotaxis). We have previously shown that the depletion of extracellular calcium blocks the directional migration of cultured human keratinocytes in an electric field (Fang et al., 1998; J Invest Dermatol 111:751-756). Here we further investigate the role of calcium influx on the directionality and migration speed of keratinocytes during electric field exposure with the use of Ca2+ channel blockers. A constant, physiological electric field strength of 100 mV/mm was imposed on the cultured cells for 1 h. To determine the role of calcium influx during galvaotaxis we tested the effects of the voltage-dependent cation channel blockers, verapamil and amiloride, as well as the inorganic Ca2+ channel blockers, Ni2+ and Gd3+ and the Ca2+ substitute, Sr2+, on the speed and directionality of keratinocyte migration during galvanotaxis. Neither amiloride (10 μM) nor verapamil (10 μM) had any effect on the galvanotaxis response. Therefore, calcium influx through amiloride-sensitive channels is not required for galvanotaxis, and membrane depolarization via K+ channel activity is also not required. In contrast, Sr2+ (5 mM), Ni2+ (1-5 mM), and Gd3+ (100 μM) all significantly inhibit the directional migratory response to some degree. While Sr2+ strongly inhibits directed migration, the cells exhibit nearly normal migration speeds. These findings suggest that calcium influx through Ca2+ channels is required for directed migration of keratinocytes during galvanotaxis and that directional migration and migration speed are probably controlled by separate mechanisms.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalJournal of Cellular Physiology
Volume193
Issue number1
DOIs
StatePublished - Oct 2002

Fingerprint

Calcium Channel Blockers
Keratinocytes
Amiloride
Calcium
Electric fields
Verapamil
Depolarization
Wound Healing
Cell Movement
Taxis Response
Cations
Cultured Cells
Poles
Cells
Membranes
Electric potential

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Calcium channel blockers inhibit galvanotaxis in human keratinocytes. / Trollinger, Donna R.; Isseroff, Roslyn Rivkah; Nuccitelli, Richard.

In: Journal of Cellular Physiology, Vol. 193, No. 1, 10.2002, p. 1-9.

Research output: Contribution to journalArticle

Trollinger, Donna R. ; Isseroff, Roslyn Rivkah ; Nuccitelli, Richard. / Calcium channel blockers inhibit galvanotaxis in human keratinocytes. In: Journal of Cellular Physiology. 2002 ; Vol. 193, No. 1. pp. 1-9.
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