Calcium-calmodulin does not alter the anion permeability of the mouse TMEM16A calcium-activated chloride channel

Yawei Yu, Ai Seon Kuan, Tsung-Yu Chen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The transmembrane protein TMEM16A forms a Ca2+-activated Cl- channel that is permeable to many anions, including SCN-, I-, Br-, Cl-, and HCO3 -, and has been implicated in various physiological functions. Indeed, controlling anion permeation through the TMEM16A channel pore may be critical in regulating the pH of exocrine fluids such as the pancreatic juice. The anion permeability of the TMEM16A channel pore has recently been reported to be modulated by Ca2+-calmodulin (CaCaM), such that the pore of the CaCaM-bound channel shows a reduced ability to discriminate between anions as measured by a shift of the reversal potential under bi-ionic conditions. Here, using a mouse TMEM16A clone that contains the two previously identified putative CaM-binding motifs, we were unable to demonstrate such CaCaM-dependent changes in the bi-ionic potential. We confirmed the activity of CaCaM used in our study by showing CaCaM modulation of the olfactory cyclic nucleotide-gated channel. We suspect that the different bi-ionic potentials that were obtained previously from whole-cell recordings in low and high intracellular [Ca2+] may result from different degrees of bi-ionic potential shift secondary to a series resistance problem, an ion accumulation effect, or both.

Original languageEnglish (US)
Pages (from-to)115-124
Number of pages10
JournalJournal of General Physiology
Volume144
Issue number1
DOIs
StatePublished - 2014

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Chloride Channels
Calmodulin
Anions
Permeability
Calcium
Cyclic Nucleotide-Gated Cation Channels
Pancreatic Juice
Patch-Clamp Techniques
Clone Cells
Ions
Proteins

ASJC Scopus subject areas

  • Physiology

Cite this

Calcium-calmodulin does not alter the anion permeability of the mouse TMEM16A calcium-activated chloride channel. / Yu, Yawei; Kuan, Ai Seon; Chen, Tsung-Yu.

In: Journal of General Physiology, Vol. 144, No. 1, 2014, p. 115-124.

Research output: Contribution to journalArticle

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