Abstract
This work determined whether mineral dissolution due to prolonged testing or storage of bone specimens in normal saline would alter their elastic modulus. In one experiment, small pieces of equine third metacarpal bone were soaked in normal saline supplemented with varying amounts of CaCl2. Changing Ca ion concentrations in the bath were monitored and the equilibrium concentration was determined. In a second experiment, the elastic moduli of twenty 4 x 10 x 100 mm equine third metacarpal beams were determined non-destructively in four-point bending. Half the beams were then soaked for 10 days in normal saline, and the other half in saline buffered to the bone mineral equilibrium point with Ca ions. Modulus measurements were repeated at 6 and 10 days. The equilibrium Ca ion concentration for bone specimens was found to be 57.5 mg l-1 The modulus of bone specimens soaked in normal saline significantly diminished 2.4%, whereas the modulus of those soaked in calcium-buffered saline did not change significantly.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1191-1194 |
| Number of pages | 4 |
| Journal | Journal of Biomechanics |
| Volume | 29 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1996 |
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Keywords
- Bone
- Elastic modulus
- Mineral dissolution
- Saline bath
ASJC Scopus subject areas
- Orthopedics and Sports Medicine
Cite this
Calcium buffering is required to maintain bone stiffness in saline solution. / Gustafson, M. B.; Martin, R. Bruce; Gibson, V.; Storms, D. H.; Stover, Susan M; Gibeling, J.; Griffin, L.
In: Journal of Biomechanics, Vol. 29, No. 9, 09.1996, p. 1191-1194.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calcium buffering is required to maintain bone stiffness in saline solution
AU - Gustafson, M. B.
AU - Martin, R. Bruce
AU - Gibson, V.
AU - Storms, D. H.
AU - Stover, Susan M
AU - Gibeling, J.
AU - Griffin, L.
PY - 1996/9
Y1 - 1996/9
N2 - This work determined whether mineral dissolution due to prolonged testing or storage of bone specimens in normal saline would alter their elastic modulus. In one experiment, small pieces of equine third metacarpal bone were soaked in normal saline supplemented with varying amounts of CaCl2. Changing Ca ion concentrations in the bath were monitored and the equilibrium concentration was determined. In a second experiment, the elastic moduli of twenty 4 x 10 x 100 mm equine third metacarpal beams were determined non-destructively in four-point bending. Half the beams were then soaked for 10 days in normal saline, and the other half in saline buffered to the bone mineral equilibrium point with Ca ions. Modulus measurements were repeated at 6 and 10 days. The equilibrium Ca ion concentration for bone specimens was found to be 57.5 mg l-1 The modulus of bone specimens soaked in normal saline significantly diminished 2.4%, whereas the modulus of those soaked in calcium-buffered saline did not change significantly.
AB - This work determined whether mineral dissolution due to prolonged testing or storage of bone specimens in normal saline would alter their elastic modulus. In one experiment, small pieces of equine third metacarpal bone were soaked in normal saline supplemented with varying amounts of CaCl2. Changing Ca ion concentrations in the bath were monitored and the equilibrium concentration was determined. In a second experiment, the elastic moduli of twenty 4 x 10 x 100 mm equine third metacarpal beams were determined non-destructively in four-point bending. Half the beams were then soaked for 10 days in normal saline, and the other half in saline buffered to the bone mineral equilibrium point with Ca ions. Modulus measurements were repeated at 6 and 10 days. The equilibrium Ca ion concentration for bone specimens was found to be 57.5 mg l-1 The modulus of bone specimens soaked in normal saline significantly diminished 2.4%, whereas the modulus of those soaked in calcium-buffered saline did not change significantly.
KW - Bone
KW - Elastic modulus
KW - Mineral dissolution
KW - Saline bath
UR - http://www.scopus.com/inward/record.url?scp=0030248070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030248070&partnerID=8YFLogxK
U2 - 10.1016/0021-9290(96)00020-6
DO - 10.1016/0021-9290(96)00020-6
M3 - Article
C2 - 8872276
AN - SCOPUS:0030248070
VL - 29
SP - 1191
EP - 1194
JO - Journal of Biomechanics
JF - Journal of Biomechanics
SN - 0021-9290
IS - 9
ER -